Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.4: cellulase

This is an abbreviated version!
For detailed information about cellulase, go to the full flat file.

Word Map on EC 3.2.1.4

Reaction

cellohexaose
+
H2O
= 2 cellotriose

Synonyms

(1->4)-beta-D-glucan 4-glucanohydrolase, 1,4-beta-D-endoglucanase, 1,4-beta-D-glucan-4-glucanohydrolase, 168cel5, 9.5 cellulase, Abscission cellulase, AEG, Ag-EGase III, AgCMCase, alkali cellulase, Alkaline cellulase, AnCel5A, AtCel5, ATEG_07420, avicelase, BC-EG70a, Bc22Cel, BCE1, BcsZ, beta-1,4-endoglucan hydrolase, beta-1,4-endoglucanase, beta-1,4-glucanase, Bgl7A, bifunctional endoglucanase/xylanase, BlCel9, BP_Cel9A, Bx-ENG-1, Bx-ENG-2, Bx-ENG-3, C4endoII, carbomethyl cellulase, Carboxymethyl cellulase, Carboxymethyl-cellulase, carboxymethylcellulase, Cat 1, Caylase, CBH45-1, cbh6A, CBHI, CBHII, CcCel6C, CEL1, Cel1 EGase, Cel12A, Cel1753, Cel28a, Cel44A, Cel45A, Cel48A, Cel5, Cel5A, cel5B, Cel5E, Cel6A, Cel6A (E2), Cel6B, Cel6C, CEL7, Cel7A, Cel7B, Cel8, Cel8A, Cel8M, Cel8Y, Cel9A, CEL9A-50, CEL9A-65, Cel9A-68, CEL9A-82, Cel9A-90, Cel9B, CEL9C1, Cel9K, Cel9M, Cel9Q, CelA, CelB, CelC2 cellulase, CelCM3, CelDR, CelE, CelF, Celf_1230, Celf_3184, CelG, CelG endoglucanase, CelI15, cell-bound bacterial cellulase, CelL15, CelL73, cellic Ctec2, cellobiohydrolase, cellobiohydrolase I, celluase A, Celluclast, celludextrinase, Cellulase, cellulase 12A, cellulase A, cellulase A 3, cellulase Cel48F, cellulase Cel9A, cellulase Cel9M, cellulase CelC2, cellulase CelE, cellulase CM3, Cellulase E1, Cellulase E2, Cellulase E4, Cellulase E5, cellulase EGX, cellulase II, cellulase III, cellulase K, Cellulase SS, cellulase T, Cellulase V1, cellulase Xf818, cellulases I, cellulases III, cellulosin AP, Cellulysin, CelP, celS, CelStrep, celVA, CelX, CenA, CenC, CfCel6A, CfCel6C, CfEG3a, CHU_1280, CHU_2103, CjCel9A, Clocel_2741, CMCase, CMCase-I, CMCax, CMcellulase, Csac_1076, Csac_1078, CSCMCase, ctCel9D-Cel44A, CTendo45, ctendo7, CtGH5, Cthe_0435, CTHT_0045780, CX-cellulase, CyPB, DCC85_10145, DK-85, Dockerin type 1, Dtur_0671, E1 endoglucanase, Econase, EfPh, EG I, EG III, EG1, EG12, EG2, EG25, EG271, EG28, EG3, EG35, EG44, EG47, EG51, Eg5a, EG60, EGA, EGase, EGase II, EGB, EGC, EGCCA, EGCCC, EGCCD, EGCCF, EGCCG, EGD, EGE, EGF, egGH45, EGH, EGI, EGII, EGII/Cel5A, EGIV, EGL, EGL 1, Egl-257, Egl1, Egl499, Egl5a, EglA, eglB, EglC, EGLII, EglS, EGM, EGPf, EGPh, EGSS, EgV, EGX, EGY, EGZ, endo-1,4-B-glucanase, endo-1,4-beta-D-glucanase, endo-1,4-beta-glucanase, endo-1,4-beta-glucanase 1, endo-1,4-beta-glucanase 2, endo-1,4-beta-glucanase E1, endo-1,4-beta-glucanase V1, endo-beta-1,3-1,4-glucanase, endo-beta-1,4-glucanase, endo-beta-1,4-glucanase 1, endo-beta-1,4-glucanase 2, endo-beta-1,4-glucanase CMCax, endo-beta-1,4-glucanase EG27, endo-beta-1,4-glucanase EG45, endo-beta-D-1,4-glucanohydrolase, endo-beta-glucanase, endo-glucanase, ENDO1, ENDO2, endocellulase, endocellulase E1, endocellulases I, endocellulases II, endocellulases III, endocellulases IV, endogenous beta-1,4-endoglucanase, endogenous cellulase, endoglucanase, endoglucanase 1, endoglucanase 35, endoglucanase 47, endoglucanase CBP105, endoglucanase Cel 12A, endoglucanase Cel 5A, endoglucanase Cel 7B, endoglucanase Cel5A, endoglucanase Cel6A, endoglucanase D, endoglucanase EG-I, endoglucanase EG25, endoglucanase EG28, endoglucanase EG44, endoglucanase EG47, endoglucanase EG51, endoglucanase EG60, endoglucanase H, endoglucanase II, endoglucanase IIa, endoglucanase IV, endoglucanase L, endoglucanase M, endoglucanase V, endoglucanase Y, endolytic cellulase, EngA, EngH, EngL, EngM, engXCA, EngY, EngZ, family 7 cellobiohydrolase, FI-CMCASE, FnCel5A, FpCel45, Fpcel45a, GE40, GE40 endoglucanase, GH12 endo-1,4-beta-glucanase, GH124 endoglucanase, GH45 endoglucanase, gh45-1, GH5 cellulase, GH5 endoglucanase, GH6 endoglucanase, GH7 endoglucanase, GH9 termite cellulase, Glu1, Glu2, glycoside hydrolase family 9 endoglucanase, GtGH45, Lp-egl-1, manganese dependent endoglucanase, Maxazyme, Meicelase, mesophilic endoglucanase, mgCel6A, More, MtGH45, nmGH45, Onozuka R10, pancellase SS, PaPopCel1, PF0854, PH1171, PttCel9A, RCE1, RCE2, Roth 3056, RtGH124, Rucel5B, Sl-cel7, Sl-cel9C1, SnEG54, SoCel5, ssgluc, SSO1354, SSO1354 enzyme, SSO1354 protein, SSO1949, SSO2534, STCE1, Sumizyme, T12-GE40, TC Serva, TeEG-I, TeEgl5A, TfCel9A, ThEG, theme C glycoside hydrolase family 9 endo-beta-glucanase, Thermoactive cellulase, thermostable carboxymethyl cellulase, THITE_2110957, TM_1525, TM_1751, umcel5G, umcel9y-1, Vul_Cel5A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.4 cellulase

Molecular Weight

Molecular Weight on EC 3.2.1.4 - cellulase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
110000
-
endoglucanase 2, non-denaturing PAGE
113000
-
endoglucanase fraction ES2U2, gel filtration
127047
x * 127047, the enzyme consists of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules, two linker sequences, and a C-terminal sequence with an unknown function, MW calculated from sequence
13000
-
endoglucanase E1, gel filtration
130000
x * 130000, mutant enzyme DELTA1-90, SDS-PAGE
15000
-
x * 15000, 1,4-beta-glucanase I, SDS-PAGE
15500
-
gel filtration
17000
-
endoglucanase fraction ES2U4, gel filtration
18000
180000
Western blot analysis, 4 intensive bands are detected. The band sizes are 180, 67, 56, and 44 kDa, 180 kDa band appears to represent protein complexes containing CEL9As formed by some type of protein-protein interaction, also detected with Zymographic analysis
183000
-
non-denaturing PAGE
185000
-
PAGE
20000
21000
22000
-
1 * 22000 + 1 * 32000, SDS-PAGE
23000
-
gel filtration
23500
-
x * 23500, endoglucanase IV, SDS-PAGE
24000
-
x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE
25000
26000
27000
28000
29000
29000 - 43000
-
PAGE
30000
31000
31200
x * 31200, SDS-PAGE
32000
32650
calculated molecular weight of mature STCE1
33000
-
x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively
33297
x * 33297, sequence calculation, x * 34000, SDS-PAGE
34000
34500
-
low speed sedimentation without reaching equilibrium
35000
35154
x * 35154, enzyme recombinantly expressed in Escherichia coli, calculated from sequence
35500
35900
x * 35900, calculated from sequence
35988
x * 35988, calculated from sequence
36000
36750
-
x * 36750, SDS-PAGE
36800
x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme
37000
37300
37508
x * 37508, calculated from sequence
38000
38400
x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation
38500
-
cellulase B
38831
-
x * 38831, calculation from nucleotide sequence
41000
41241
x * 41241, calculated from sequuence
41500
42000
42100
-
x * 42100, calculated from sequence
43000
43970
theoretically, EGPhDELTAC5, truncated form of the enzyme
44000
45000
45561
x * 45561, wild-type enzyme (389 amino acids) expressed with both N(28 residues)- and C(42 residues)-terminal truncations (full length EGPh contains 458 amino acids) with a signal peptide sequence of 28 residues and a C-terminal region involved in anchoring
46000
46500
-
x * 46500, endoglucanase I, SDS-PAGE
46700
-
x * 46700, CenA
47000
47090
calculated molecular weight, C-terminal His-tagged form
48160
calculated molecular weight, wild-type
50020
calculated, with cellulose-binding module
51000
51400
x * 51400 and x * 57100, without and with signal peptide, respectively, SDS-PAGE
52000
52700
-
x * 52700, calculated from sequence
53000
53780
calculated, with cellulose-binding module
54000
54618
-
x * 54618, the enzyme contains a catalytic domain belonging to family 9 and a dockering domain, it is a component of the cellulosome, calculation from nucleotide sequence
55000
55118
-
x * 55118, calculated from sequence
56000
56463
-
x * 56463, calculation from nucleotide sequence
56500
-
gel filtration
57000
57100
x * 51400 and x * 57100, without and with signal peptide, respectively, SDS-PAGE
58000
585000
-
x * 585000, endoglucanase III, SDS-PAGE
59000
-
1 * 59000, SDS-PAGE
60000
61000
62000
62300
x * 62300, calculated, x * 60000, SDS-PAGE
62360
calculated molecular weight
62600
1 * 62600, calculated
64000
65000
66000
67000
67103
x * 67103, calculation from nucleotide sequence
67800
Thermochaetoides thermophila
-
-
68000
-
x * 68000, SDS-PAGE
69800
Thermochaetoides thermophila
-
gel filtration
70000
71000
72000
-
recombinant Cel7B protein, could be hyperglycosylated, SDS-PAGE
75000
-
gel filtration
75500
gel filtration, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
76000
-
x * 76000, SDS-PAGE
78000
78100
-
x * 78100, SDS-PAGE
80000
82000
83000
-
endoglucanase I, equilibrium sedimentation
89000
-
SDS-PAGE
93000
-
endoglucanase fraction ES2U3C2, gel filtration
93800
x * 93800, calculation from nucleotide sequence
94000
95000
-
SDS-PAGE
96000
96798
x * 96798, calculated from sequence
98466
x * 98466, calculated
additional information
-
chemical deglycosylation results in a heterogeneous protein band with a molecular mass ranging from 48 to 56 kDa