3.2.1.4: cellulase This is an abbreviated version! For detailed information about cellulase, go to the full flat file .
Reaction
cellohexaose +
H2O =
2 cellotriose
Synonyms (1->4)-beta-D-glucan 4-glucanohydrolase, 1,4-beta-D-endoglucanase, 1,4-beta-D-glucan-4-glucanohydrolase, 168cel5, 9.5 cellulase, Abscission cellulase, AEG, Ag-EGase III, AgCMCase, alkali cellulase, Alkaline cellulase, AnCel5A, AtCel5, ATEG_07420, avicelase, BC-EG70a, Bc22Cel, BCE1, BcsZ, beta-1,4-endoglucan hydrolase, beta-1,4-endoglucanase, beta-1,4-glucanase, Bgl7A, bifunctional endoglucanase/xylanase, BlCel9, BP_Cel9A, Bx-ENG-1, Bx-ENG-2, Bx-ENG-3, C4endoII, carbomethyl cellulase, Carboxymethyl cellulase, Carboxymethyl-cellulase, carboxymethylcellulase, Cat 1, Caylase, CBH45-1, cbh6A, CBHI, CBHII, CcCel6C, CEL1, Cel1 EGase, Cel12A, Cel1753, Cel28a, Cel44A, Cel45A, Cel48A, Cel5, Cel5A, cel5B, Cel5E, Cel6A, Cel6A (E2), Cel6B, Cel6C, CEL7, Cel7A, Cel7B, Cel8, Cel8A, Cel8M, Cel8Y, Cel9A, CEL9A-50, CEL9A-65, Cel9A-68, CEL9A-82, Cel9A-90, Cel9B, CEL9C1, Cel9K, Cel9M, Cel9Q, CelA, CelB, CelC2 cellulase, CelCM3, CelDR, CelE, CelF, Celf_1230, Celf_3184, CelG, CelG endoglucanase, CelI15, cell-bound bacterial cellulase, CelL15, CelL73, cellic Ctec2, cellobiohydrolase, cellobiohydrolase I, celluase A, Celluclast, celludextrinase, Cellulase, cellulase 12A, cellulase A, cellulase A 3, cellulase Cel48F, cellulase Cel9A, cellulase Cel9M, cellulase CelC2, cellulase CelE, cellulase CM3, Cellulase E1, Cellulase E2, Cellulase E4, Cellulase E5, cellulase EGX, cellulase II, cellulase III, cellulase K, Cellulase SS, cellulase T, Cellulase V1, cellulase Xf818, cellulases I, cellulases III, cellulosin AP, Cellulysin, CelP, celS, CelStrep, celVA, CelX, CenA, CenC, CfCel6A, CfCel6C, CfEG3a, CHU_1280, CHU_2103, CjCel9A, Clocel_2741, CMCase, CMCase-I, CMCax, CMcellulase, Csac_1076, Csac_1078, CSCMCase, ctCel9D-Cel44A, CTendo45, ctendo7, CtGH5, Cthe_0435, CTHT_0045780, CX-cellulase, CyPB, DCC85_10145, DK-85, Dockerin type 1, Dtur_0671, E1 endoglucanase, Econase, EfPh, EG I, EG III, EG1, EG12, EG2, EG25, EG271, EG28, EG3, EG35, EG44, EG47, EG51, Eg5a, EG60, EGA, EGase, EGase II, EGB, EGC, EGCCA, EGCCC, EGCCD, EGCCF, EGCCG, EGD, EGE, EGF, egGH45, EGH, EGI, EGII, EGII/Cel5A, EGIV, EGL, EGL 1, Egl-257, Egl1, Egl499, Egl5a, EglA, eglB, EglC, EGLII, EglS, EGM, EGPf, EGPh, EGSS, EgV, EGX, EGY, EGZ, endo-1,4-B-glucanase, endo-1,4-beta-D-glucanase, endo-1,4-beta-glucanase, endo-1,4-beta-glucanase 1, endo-1,4-beta-glucanase 2, endo-1,4-beta-glucanase E1, endo-1,4-beta-glucanase V1, endo-beta-1,3-1,4-glucanase, endo-beta-1,4-glucanase, endo-beta-1,4-glucanase 1, endo-beta-1,4-glucanase 2, endo-beta-1,4-glucanase CMCax, endo-beta-1,4-glucanase EG27, endo-beta-1,4-glucanase EG45, endo-beta-D-1,4-glucanohydrolase, endo-beta-glucanase, endo-glucanase, ENDO1, ENDO2, endocellulase, endocellulase E1, endocellulases I, endocellulases II, endocellulases III, endocellulases IV, endogenous beta-1,4-endoglucanase, endogenous cellulase, endoglucanase, endoglucanase 1, endoglucanase 35, endoglucanase 47, endoglucanase CBP105, endoglucanase Cel 12A, endoglucanase Cel 5A, endoglucanase Cel 7B, endoglucanase Cel5A, endoglucanase Cel6A, endoglucanase D, endoglucanase EG-I, endoglucanase EG25, endoglucanase EG28, endoglucanase EG44, endoglucanase EG47, endoglucanase EG51, endoglucanase EG60, endoglucanase H, endoglucanase II, endoglucanase IIa, endoglucanase IV, endoglucanase L, endoglucanase M, endoglucanase V, endoglucanase Y, endolytic cellulase, EngA, EngH, EngL, EngM, engXCA, EngY, EngZ, family 7 cellobiohydrolase, FI-CMCASE, FnCel5A, FpCel45, Fpcel45a, GE40, GE40 endoglucanase, GH12 endo-1,4-beta-glucanase, GH124 endoglucanase, GH45 endoglucanase, gh45-1, GH5 cellulase, GH5 endoglucanase, GH6 endoglucanase, GH7 endoglucanase, GH9 termite cellulase, Glu1, Glu2, glycoside hydrolase family 9 endoglucanase, GtGH45, Lp-egl-1, manganese dependent endoglucanase, Maxazyme, Meicelase, mesophilic endoglucanase, mgCel6A, More, MtGH45, nmGH45, Onozuka R10, pancellase SS, PaPopCel1, PF0854, PH1171, PttCel9A, RCE1, RCE2, Roth 3056, RtGH124, Rucel5B, Sl-cel7, Sl-cel9C1, SnEG54, SoCel5, ssgluc, SSO1354, SSO1354 enzyme, SSO1354 protein, SSO1949, SSO2534, STCE1, Sumizyme, T12-GE40, TC Serva, TeEG-I, TeEgl5A, TfCel9A, ThEG, theme C glycoside hydrolase family 9 endo-beta-glucanase, Thermoactive cellulase, thermostable carboxymethyl cellulase, THITE_2110957, TM_1525, TM_1751, umcel5G, umcel9y-1, Vul_Cel5A
ECTree
Molecular Weight
Molecular Weight on EC 3.2.1.4 - cellulase
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110000
-
endoglucanase 2, non-denaturing PAGE
113000
-
endoglucanase fraction ES2U2, gel filtration
127047
x * 127047, the enzyme consists of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules, two linker sequences, and a C-terminal sequence with an unknown function, MW calculated from sequence
13000
-
endoglucanase E1, gel filtration
130000
x * 130000, mutant enzyme DELTA1-90, SDS-PAGE
15000
-
x * 15000, 1,4-beta-glucanase I, SDS-PAGE
17000
-
endoglucanase fraction ES2U4, gel filtration
180000
Western blot analysis, 4 intensive bands are detected. The band sizes are 180, 67, 56, and 44 kDa, 180 kDa band appears to represent protein complexes containing CEL9As formed by some type of protein-protein interaction, also detected with Zymographic analysis
183000
-
non-denaturing PAGE
22000
-
1 * 22000 + 1 * 32000, SDS-PAGE
23500
-
x * 23500, endoglucanase IV, SDS-PAGE
24000
-
x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE
31200
x * 31200, SDS-PAGE
32650
calculated molecular weight of mature STCE1
33000
-
x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively
33297
x * 33297, sequence calculation, x * 34000, SDS-PAGE
34500
-
low speed sedimentation without reaching equilibrium
35154
x * 35154, enzyme recombinantly expressed in Escherichia coli, calculated from sequence
35900
x * 35900, calculated from sequence
35988
x * 35988, calculated from sequence
36750
-
x * 36750, SDS-PAGE
36800
x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme
37508
x * 37508, calculated from sequence
38400
x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation
38831
-
x * 38831, calculation from nucleotide sequence
41241
x * 41241, calculated from sequuence
42100
-
x * 42100, calculated from sequence
43970
theoretically, EGPhDELTAC5, truncated form of the enzyme
45561
x * 45561, wild-type enzyme (389 amino acids) expressed with both N(28 residues)- and C(42 residues)-terminal truncations (full length EGPh contains 458 amino acids) with a signal peptide sequence of 28 residues and a C-terminal region involved in anchoring
46500
-
x * 46500, endoglucanase I, SDS-PAGE
47090
calculated molecular weight, C-terminal His-tagged form
48160
calculated molecular weight, wild-type
50020
calculated, with cellulose-binding module
51400
x * 51400 and x * 57100, without and with signal peptide, respectively, SDS-PAGE
52700
-
x * 52700, calculated from sequence
53780
calculated, with cellulose-binding module
54618
-
x * 54618, the enzyme contains a catalytic domain belonging to family 9 and a dockering domain, it is a component of the cellulosome, calculation from nucleotide sequence
55118
-
x * 55118, calculated from sequence
56463
-
x * 56463, calculation from nucleotide sequence
57100
x * 51400 and x * 57100, without and with signal peptide, respectively, SDS-PAGE
585000
-
x * 585000, endoglucanase III, SDS-PAGE
59000
-
1 * 59000, SDS-PAGE
62300
x * 62300, calculated, x * 60000, SDS-PAGE
62360
calculated molecular weight
62600
1 * 62600, calculated
67103
x * 67103, calculation from nucleotide sequence
67800
Thermochaetoides thermophila
-
-
68000
-
x * 68000, SDS-PAGE
69800
Thermochaetoides thermophila
-
gel filtration
72000
-
recombinant Cel7B protein, could be hyperglycosylated, SDS-PAGE
75500
gel filtration, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
76000
-
x * 76000, SDS-PAGE
78100
-
x * 78100, SDS-PAGE
83000
-
endoglucanase I, equilibrium sedimentation
93000
-
endoglucanase fraction ES2U3C2, gel filtration
93800
x * 93800, calculation from nucleotide sequence
96798
x * 96798, calculated from sequence
98466
x * 98466, calculated
additional information
-
chemical deglycosylation results in a heterogeneous protein band with a molecular mass ranging from 48 to 56 kDa
100000
-
PAGE
100000
-
endoglucanase 2, gel filtration
18000
gel filtration
20000
-
20000
-
x * 20000, SDS-PAGE
21000
-
gel filtration
21000
-
1,4-beta-glucanase I, gel filtration
21000
-
x * 21000, endoglucanase II, SDS-PAGE
25000
-
25000
-
cellulase I, gel filtration
25000
Bx-ENG-1 shows a single band with a molecular size of 25 kDa when 2-mercaptethanol is added to the SDS-PAGE sample
25000
-
x * 25000, SDS-PAGE
25000
-
4 * 25000, SDS-PAGE
25000
-
x * 25000, endoglucanase EG25, SDS-PAGE
26000
-
gel filtration
26000
-
x * 26000, SDS-PAGE
27000
x * 27000, SDS-PAGE
27000
-
1 * 27000, SDS-PAGE
27000
-
x * 27000, endo-beta-1,4-glucanase EG27, SDS-PAGE
27000
x * 27000, recombinant CelL73, SDS-PAGE
27000
-
x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE
28000
-
endoglucanase B
28000
x * 28000, SDS-PAGE
28000
-
x * 28000, endoglucanase EG28, SDS-PAGE
28000
-
x * 28000, calculated, x * 28000, and x * 33000, SDS-PAGE of unglycosylated and glycosylated protein, respectively
29000
Lenzites trabea
-
gel filtration
29000
-
x * 29000, catalytic domain, SDS-PAGE
29000
x * 29000, chimeric fusion protein CelA-SSO1949-CelA consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA, SDS-PAGE
29000
x * 29000, chimeric fusion protein CelA-SSO1949-CelA consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA, SDS-PAGE
30000
-
gel filtration
30000
-
cellulase II-A, gel filtration
30000
-
endoglucanase fraction ES3, gel filtration
30000
-
x * 30000, SDS-PAGE
30000
-
x * 30000, endoglucanase IV, SDS-PAGE
31000
-
endoglucanase E4, gel filtration
31000
-
C-terminus truncation mutant Egl330, calculated
31000
-
x * 31000, SDS-PAGE
31000
-
x * 31000, SDS-PAGE
32000
-
x * 32000, SDS-PAGE
32000
-
1 * 22000 + 1 * 32000, SDS-PAGE
34000
SDS-PAGE
34000
-
cellulase III, gel filtration
34000
x * 33297, sequence calculation, x * 34000, SDS-PAGE
34000
-
gel filtration, cellulase III
35000
-
x * 35000, endoglucanase Y, SDS-PAGE
35000
-
x * 35000, endoglucanase 35, SDS-PAGE
35000
-
SDS-PAGE, endocellulases I, endocellulases II, endocellulases III, endocellulases IV
35500
-
2 * 35500, endo-beta-1,4-glucanase E1, E2 and E3, SDS-PAGE
35500
x * 35500, enzyme recombinantly expressed in Escherichia coli, SDS-PAGE
36000
-
gel filtration
36000
-
wild-type enzyme and mutant enzyme CRRmt24
36000
-
C-terminus truncation mutant Egl330, calculated
36000
Thermochaetoides thermophila
-
1 * 36000, SDS-PAGE
36000
-
x * 36000, SDS-PAGE
36000
difference to 56000 due to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE
36000
difference to 56000 duet to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE
37000
x * 37000, SDS-PAGE
37000
x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme
37300
-
x * 37300, endoglucanase III, SDS-PAGE
37300
x * 37300, calculated from sequence
38000
Thermochaetoides thermophila
-
gel filtration
38000
-
endoglucanase fraction ES2U3C1, gel filtration
38000
x * 38000, SDS-PAGE
38000
-
x * 38000, SDS-PAGE
40000
-
gel filtration
40000
EGPhDELTAC5, SDS-PAGE
40000
main active silver-stained band at 57.0 kDa and a light activity at 40.0 kDa (also active towards xylan and carboxymethylcellulose, represents the almost totally deglycosylated SSO1354 protein), SDS-PAGE. Periodic acid Schiff (PAS) staining: only the highest zymogram active band (57.0 kDa) is revealed
40000
-
x * 40000, SDS-PAGE
40000
-
x * 40000, SDS-PAGE
40000
-
x * 40000, SDS-PAGE
40000
-
x * 40000, SDS-PAGE
40000
x * 40000, SDS-PAGE
40000
-
x * 40000, CMCase 1, SDS-PAGE
40000
-
x * 40000, enzyme form EGB, SDS-PAGE
40000
x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation
41000
Thermochaetoides thermophila
-
-
41000
-
1 * 41000, SDS-PAGE
41000
x * 41000, SDS-PAGE
41000
-
x * 41000, endo-beta-1,4-glucanase component YEG2, SDS-PAGE
41500
-
gel filtration
41500
-
x * 41500, SDS-PAGE
42000
-
cellulase A
42000
-
4 * 42000, SDS-PAGE
42000
-
x * 42000, SDS-PAGE
42000
-
x * 42000, SDS-PAGE
42000
-
1 * 42000, SDS-PAGE
42000
-
x * 42000, recombinant enzyme, SDS-PAGE
42000
-
x * 42000, endo-beta-1,4-glucanase component YEG1, SDS-PAGE
42000
x * 42000, truncated enzyme form expressed in Escherichia coli
43000
gel filtration
43000
-
cellulase II-B, gel filtration
43000
wild-type protein, SDS-PAGE
43000
x * 43000, SDS-PAGE
43000
x * 43000, SDS-PAGE and calculated
44000
-
gel filtration
44000
Western blot analysis, 4 intensive bands are detected. The band sizes are 180, 67, 56, and 44 kDa, CEL9A-50 retaining intramolecular disulfide bonds, also detected with Zymographic analysis
44000
-
x * 44000, SDS-PAGE
44000
-
x * 44000, endoglucanase EG44, SDS-PAGE
45000
-
gel filtration
45000
-
x * 45000, SDS-PAGE
45000
-
x * 45000, SDS-PAGE
45000
-
x * 45000, SDS-PAGE
45000
-
x * 45000, endoglucanase II, SDS-PAGE
45000
-
x * 45000, endo-beta-1,4-glucanase EG45, SDS-PAGE
46000
-
SDS-PAGE
46000
-
theoretical molecular mass of unglycosylated mature Cel7B
46000
-
1 * 46000, SDS-PAGE
47000
-
gel fitration
47000
Thermochaetoides thermophila
calculated from sequence
47000
-
Western blot, SDS-PAGE
47000
-
x * 47000, SDS-PAGE
47000
-
1 * 47000, SDS-PAGE
47000
-
x * 47000, endoglucanase EG47, SDS-PAGE
47000
-
x * 47000, endoglucanase 47, SDS-PAGE
48000
-
gel filtration
48000
-
enoglucanase E3a, gel filtration
48000
-
cellulase II, gel filtration
48000
-
endoglucanase E3a and E3b, gel filtration
48000
-
gel filtration, pH 8.5
48000
-
1 * 48000, SDS-PAGE
48000
-
x * 48000, CMCase 2, SDS-PAGE
48000
-
x * 48000, endoglucanase 2, SDS-PAGE
48000
x * 48000, deglycosylated Bgl7A, SDS-PAGE, x * 60000-80000, recombinant glycosylated enzyme, SDS-PAGE
48000
-
gel filtration, cellulase II
50000
-
50000
3 intensive bands are detected. The band sizes are 82, 65, and 50 kDa, SDS-PAGE, CEL9A-50 may be a posttranslationally modified form of CEL9A-82 or CEL9A-65
50000
-
Egl499 wild-type, SDS-PAGE
50000
-
theoretical molecular mass of native Cel7B is only about 50 to 55 kDa
50000
-
x * 50000, endoglucanase IV, SDS-PAGE
50000
-
x * 50000, enzymatically active domain, SDS-PAGE
50000
-
x * 50000, endoglucanase I, SDS-PAGE
51000
-
-
51000
-
x * 51000, endoglucanase EG51, SDS-PAGE
52000
-
-
52000
-
endo-beta-1,4-glucanase 2
52000
-
x * 52000, endoglucanase VI, SDS-PAGE
52000
-
x * 52000, endoglucanase I, SDS-PAGE
52000
x * 52000, calculated
52000
x * 52000, recombinant extracellular enzyme, SDS-PAGE
53000
-
gel filtration
53000
-
endo-beta-1,4-glucanase 1
54000
-
x * 54000, SDS-PAGE
54000
-
x * 54000, SDS-PAGE
54000
x * 54000, recombinant CelL15, SDS-PAGE
55000
SDS-PAGE
55000
-
Egl499 wild-type, SDS-PAGE
55000
x * 55000, SDS-PAGE
55000
x * 55000, SDS-PAGE
56000
-
gel filtration
56000
4 intensive bands are detected. The band sizes are 180, 67, 56, and 44 kDa, Western blot analysis, CEL9A-65 retaining intramolecular disulfide bonds
56000
-
x * 56000, SDS-PAGE
56000
-
x * 56000, SDS-PAGE
56000
-
x * 56000, enzyme form EGA, SDS-PAGE
56000
difference to 36000 due to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE
56000
difference to 36000 duet to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE
57000
main active silver-stained band at 57.0 kDa and a light activity at 40.0 kDa (also active towards xylan and carboxymethylcellulose, represents the almost totally deglycosylated SSO1354 protein), SDS-PAGE. Periodic acid Schiff (PAS) staining: only the highest zymogram active band (57.0 kDa) is revealed
57000
-
x * 57000, SDS-PAGE
57000
-
x * 57000, endoglucanase V, SDS-PAGE
58000
-
x * 58000, SDS-PAGE
58000
-
x * 58000, SDS-PAGE
60000
gel filtration
60000
-
endoglucanase 1b, non-denaturing PAGE
60000
x * 60000, SDS-PAGE
60000
-
x * 60000, endoglucanase EG60, SDS-PAGE
60000
x * 62300, calculated, x * 60000, SDS-PAGE
61000
-
endoglucanase fraction ES2U1, gel filtration
62000
-
x * 62000, SDS-PAGE
62000
x * 62000, SDS-PAGE
64000
-
gel filtration
64000
-
endoglucanase fraction ES1, gel filtration
64000
-
endoglucanase 1b, gel filtration
64000
Bacillus cellulyticus K-12
-
1 * 64000, SDS-PAGE
64000
-
x * 64000, SDS-PAGE
64000
-
x * 64000, recombinant truncated mutant, SDS-PAGE
65000
Bacillus cellulyticus K-12
-
gel filtration
65000
-
equilibrium sedimentation
65000
3 intensive bands are detected. The band sizes are 82, 65, and 50 kDa, SDS-PAGE, CEL9A-65
65000
-
x * 65000, enzyme form EGD, SDS-PAGE
66000
-
1 * 66000, SDS-PAGE
66000
-
x * 66000, enzyme form EGB, SDS-PAGE
67000
gel filtration
67000
Western blot analysis, 4 intensive bands are detected. The band sizes are 180, 67, 56, and 44 kDa, CEL9A-82 retaining intramolecular disulfide bonds, also detected with Zymographic analysis
70000
-
gel filtration
70000
-
x * 70000, 1,4-beta-glucanase II, SDS-PAGE
71000
-
gel filtration
71000
-
1,4-beta-glucanase II, gel filtration
78000
-
cellulase I, gel filtration
78000
-
gel filtration, cellulase I
80000
-
x * 80000, SDS-PAGE
80000
-
x * 80000, SDS-PAGE
82000
3 intensive bands are detected. The band sizes are 82, 65, and 50 kDa, SDS-PAGE, CEL9A-82 may be a highly glycosylated form of CEL9A-65
82000
-
1 * 82000, SDS-PAGE
94000
-
endoglucanase I, non-denaturing PAGE
94000
x * 94000, SDS-PAGE
94000
-
1 * 94000, endoglucanase E1, SDS-PAGE
94000
x * 94000, recombinant enzyme, SDS-PAGE
96000
-
gel filtration
96000
-
x * 96000, SDS-PAGE