Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, 82.3% residual activity, 50%, 48 h, crude enzyme preparation | Gracilibacillus sp. | |
EDTA | 5 mM, 17.9% residual activity, 50%, 48 h, crude enzyme preparation | Gracilibacillus sp. | |
Hg2+ | 5 mM, 21.9% residual activity, 50%, 48 h, crude enzyme preparation | Gracilibacillus sp. | |
Mn2+ | 5 mM, 82.5% residual activity, 50%, 48 h, crude enzyme preparation | Gracilibacillus sp. | |
PMSF | 5 mM, 10.1% residual activity, 50%, 48 h, crude enzyme preparation | Gracilibacillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Gracilibacillus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | more than 60% activity is retained after incubation with 0-25% NaCl for 48 h | Gracilibacillus sp. |
Organic Solvent | Comment | Organism |
---|---|---|
1-butanol | 50%, 48 h, crude enzyme preparation, 58.3% residual activity | Gracilibacillus sp. |
Acetone | 50%, 48 h, crude enzyme preparation, 57.6% residual activity | Gracilibacillus sp. |
acetonitrile | 50%, 48 h, crude enzyme preparation, 31.2% residual activity | Gracilibacillus sp. |
DMSO | 50%, 48 h, crude enzyme preparation, 41% residual activity | Gracilibacillus sp. |
Ethanol | 50%, 48 h, crude enzyme preparation, 25.1% residual activity | Gracilibacillus sp. |
hexane | 50%, 48 h, crude enzyme preparation, 117.1% residual activity | Gracilibacillus sp. |
Methanol | 50%, 48 h, crude enzyme preparation, 31.5% residual activity | Gracilibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gracilibacillus sp. | - |
- |
- |
Gracilibacillus sp. SK1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
218.9 | - |
substrate cellobiose, crude enzyme preparation, pH 8.0, 60°C | Gracilibacillus sp. |
234.6 | - |
substrate cotton straw, crude enzyme preparation, pH 8.0, 60°C | Gracilibacillus sp. |
257.8 | - |
substrate wheat straw, crude enzyme preparation, pH 8.0, 60°C | Gracilibacillus sp. |
315.2 | - |
substrate 4-nitrophenyl beta-D-glucopyranoside, crude enzyme preparation, pH 8.0, 60°C | Gracilibacillus sp. |
354.1 | - |
substrate rice straw, crude enzyme preparation, pH 8.0, 60°C | Gracilibacillus sp. |
429.7 | - |
substrate corn stover, crude enzyme preparation, pH 8.0, 60°C | Gracilibacillus sp. |
510.8 | - |
substrate carboxymethyl cellulose, crude enzyme preparation, pH 8.0, 60°C | Gracilibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Gracilibacillus sp. | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Gracilibacillus sp. SK1 | 4-nitrophenol + D-glucose | - |
? | |
carboxymethyl cellulose + H2O | - |
Gracilibacillus sp. | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Gracilibacillus sp. SK1 | ? | - |
? | |
cellobiose + H2O | - |
Gracilibacillus sp. | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Gracilibacillus sp. SK1 | 2 D-glucose | - |
? | |
corn stover + H2O | - |
Gracilibacillus sp. | ? | - |
? | |
cotton straw + H2O | - |
Gracilibacillus sp. | ? | - |
? | |
rice straw + H2O | - |
Gracilibacillus sp. | ? | - |
? | |
rice straw + H2O | - |
Gracilibacillus sp. SK1 | ? | - |
? | |
wheat straw + H2O | - |
Gracilibacillus sp. | ? | - |
? | |
wheat straw + H2O | - |
Gracilibacillus sp. SK1 | ? | - |
? |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
48 h, complete loss of activity, crude cellulase preparation | Gracilibacillus sp. |
7 | 9 | 48 h, high stability, crude cellulase preparation | Gracilibacillus sp. |
Organism | Comment | Expression |
---|---|---|
Gracilibacillus sp. | extracellular cellulase is expressed from the early-exponential phase (4 h) and reaches a maximum level during the stationary phase of bacterial growth (20 h) | additional information |
Gracilibacillus sp. | expression is induced in presence of carboxymethyl cellulose | up |
General Information | Comment | Organism |
---|---|---|
physiological function | Gracilibacillus sp. secretes six different endoglucanases | Gracilibacillus sp. |