Application | Comment | Organism |
---|---|---|
industry | enzyme Bc22Cel is a potential and useful candidate for industrial applications, such as the bioconversion of sugarcane bagasse to its derivatives | Bacillus sp. SR22 |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, analysis, and comparisons, phylogenetic tree | Bacillus sp. SR22 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km and Vmax are 0.704 mg/ml and 0.02985 mmol/ml/min, respectively | Bacillus sp. SR22 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Bacillus sp. SR22 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | the purified Bc22Cel shows a considerable halophilic property, being able to maintain more than 70% of residual activity when pre-incubated with 1.5 M NaCl for 1 h | Bacillus sp. SR22 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. SR22 | - |
isolated from Siderastrea stellata tissue samples collected at Cabo Branco shore, Brasil | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity by ammonium sulfate fractionation and gel filtration | Bacillus sp. SR22 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | growth of the strain SR22 marine isolate on CMC and sugarcane bagasse as sole carbon source, compared on solid media at different salinity ranges, overview | Bacillus sp. SR22 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43.02 | - |
purified native enzyme, pH 6.5, 60°C | Bacillus sp. SR22 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Bacillus sp. SR22 | ? | - |
? | |
sugarcane bagasse + H2O | - |
Bacillus sp. SR22 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, about, SDS-PAGE | Bacillus sp. SR22 |
Synonyms | Comment | Organism |
---|---|---|
Bc22Cel | - |
Bacillus sp. SR22 |
endo-beta-glucanase | - |
Bacillus sp. SR22 |
endoglucanase | - |
Bacillus sp. SR22 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. SR22 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bacillus sp. SR22 |
General Information | Comment | Organism |
---|---|---|
physiological function | endo-beta-glucanases (E.C. 3.2.1.4) are able to cleave the cellulosic structure in internal sites, generating a large number of ends where exo-beta-glucanases (E.C. 3.2.1.91 and EC 3.2.1.176) are able to act on degrading the reducing and non-reducing ends of cellulose to small glucose oligomers (cellobiose and cellodextrins), which in turn are hydrolyzed to single glucose units by enzymes denominated by beta-glycosidases (E.C. 3.2.1.21) | Bacillus sp. SR22 |