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Literature summary for 3.2.1.4 extracted from

  • Vu, V.H.; Kim, K.
    Improvement of cellulase activity using error-prone rolling circle amplification and site-directed mutagenesis (2012), J. Microbiol. Biotechnol., 22, 607-613.
    View publication on PubMed
Search for more literature for 3.2.1.4

Protein Variants

Protein Variants Comment Organism
E289V mutation identified by error-prone rolling circle amplification. Mutation results in a 7.93-fold increase in its enzymatic activity Bacillus amyloliquefaciens
additional information improvement of endoglucanase activity by utilizing error-prone rolling circle amplification, supplemented with 1.7 mM MnCl2. The procedure generates random mutations in the Bacillus amyloliquefaciens endoglucanase gene with a frequency of 10 mutations per kilobase Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-
Bacillus amyloliquefaciens DL-3
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
 wild-type, pH 4.8, 50°C Bacillus amyloliquefaciens
5.55
-
 mutant E289V, pH 4.8, 55°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
carboxymethyl cellulose + H2O
-
 Bacillus amyloliquefaciens DL-3 ?
-
 ?