3.2.1.4: cellulase This is an abbreviated version! For detailed information about cellulase, go to the full flat file .
Reaction
cellohexaose +
H2O =
2 cellotriose
Synonyms (1->4)-beta-D-glucan 4-glucanohydrolase, 1,4-beta-D-endoglucanase, 1,4-beta-D-glucan-4-glucanohydrolase, 168cel5, 9.5 cellulase, Abscission cellulase, AEG, Ag-EGase III, AgCMCase, alkali cellulase, Alkaline cellulase, AnCel5A, AtCel5, ATEG_07420, avicelase, BC-EG70a, Bc22Cel, BCE1, BcsZ, beta-1,4-endoglucan hydrolase, beta-1,4-endoglucanase, beta-1,4-glucanase, Bgl7A, bifunctional endoglucanase/xylanase, BlCel9, BP_Cel9A, Bx-ENG-1, Bx-ENG-2, Bx-ENG-3, C4endoII, carbomethyl cellulase, Carboxymethyl cellulase, Carboxymethyl-cellulase, carboxymethylcellulase, Cat 1, Caylase, CBH45-1, cbh6A, CBHI, CBHII, CcCel6C, CEL1, Cel1 EGase, Cel12A, Cel1753, Cel28a, Cel44A, Cel45A, Cel48A, Cel5, Cel5A, cel5B, Cel5E, Cel6A, Cel6A (E2), Cel6B, Cel6C, CEL7, Cel7A, Cel7B, Cel8, Cel8A, Cel8M, Cel8Y, Cel9A, CEL9A-50, CEL9A-65, Cel9A-68, CEL9A-82, Cel9A-90, Cel9B, CEL9C1, Cel9K, Cel9M, Cel9Q, CelA, CelB, CelC2 cellulase, CelCM3, CelDR, CelE, CelF, Celf_1230, Celf_3184, CelG, CelG endoglucanase, CelI15, cell-bound bacterial cellulase, CelL15, CelL73, cellic Ctec2, cellobiohydrolase, cellobiohydrolase I, celluase A, Celluclast, celludextrinase, Cellulase, cellulase 12A, cellulase A, cellulase A 3, cellulase Cel48F, cellulase Cel9A, cellulase Cel9M, cellulase CelC2, cellulase CelE, cellulase CM3, Cellulase E1, Cellulase E2, Cellulase E4, Cellulase E5, cellulase EGX, cellulase II, cellulase III, cellulase K, Cellulase SS, cellulase T, Cellulase V1, cellulase Xf818, cellulases I, cellulases III, cellulosin AP, Cellulysin, CelP, celS, CelStrep, celVA, CelX, CenA, CenC, CfCel6A, CfCel6C, CfEG3a, CHU_1280, CHU_2103, CjCel9A, Clocel_2741, CMCase, CMCase-I, CMCax, CMcellulase, Csac_1076, Csac_1078, CSCMCase, ctCel9D-Cel44A, CTendo45, ctendo7, CtGH5, Cthe_0435, CTHT_0045780, CX-cellulase, CyPB, DCC85_10145, DK-85, Dockerin type 1, Dtur_0671, E1 endoglucanase, Econase, EfPh, EG I, EG III, EG1, EG12, EG2, EG25, EG271, EG28, EG3, EG35, EG44, EG47, EG51, Eg5a, EG60, EGA, EGase, EGase II, EGB, EGC, EGCCA, EGCCC, EGCCD, EGCCF, EGCCG, EGD, EGE, EGF, egGH45, EGH, EGI, EGII, EGII/Cel5A, EGIV, EGL, EGL 1, Egl-257, Egl1, Egl499, Egl5a, EglA, eglB, EglC, EGLII, EglS, EGM, EGPf, EGPh, EGSS, EgV, EGX, EGY, EGZ, endo-1,4-B-glucanase, endo-1,4-beta-D-glucanase, endo-1,4-beta-glucanase, endo-1,4-beta-glucanase 1, endo-1,4-beta-glucanase 2, endo-1,4-beta-glucanase E1, endo-1,4-beta-glucanase V1, endo-beta-1,3-1,4-glucanase, endo-beta-1,4-glucanase, endo-beta-1,4-glucanase 1, endo-beta-1,4-glucanase 2, endo-beta-1,4-glucanase CMCax, endo-beta-1,4-glucanase EG27, endo-beta-1,4-glucanase EG45, endo-beta-D-1,4-glucanohydrolase, endo-beta-glucanase, endo-glucanase, ENDO1, ENDO2, endocellulase, endocellulase E1, endocellulases I, endocellulases II, endocellulases III, endocellulases IV, endogenous beta-1,4-endoglucanase, endogenous cellulase, endoglucanase, endoglucanase 1, endoglucanase 35, endoglucanase 47, endoglucanase CBP105, endoglucanase Cel 12A, endoglucanase Cel 5A, endoglucanase Cel 7B, endoglucanase Cel5A, endoglucanase Cel6A, endoglucanase D, endoglucanase EG-I, endoglucanase EG25, endoglucanase EG28, endoglucanase EG44, endoglucanase EG47, endoglucanase EG51, endoglucanase EG60, endoglucanase H, endoglucanase II, endoglucanase IIa, endoglucanase IV, endoglucanase L, endoglucanase M, endoglucanase V, endoglucanase Y, endolytic cellulase, EngA, EngH, EngL, EngM, engXCA, EngY, EngZ, family 7 cellobiohydrolase, FI-CMCASE, FnCel5A, FpCel45, Fpcel45a, GE40, GE40 endoglucanase, GH12 endo-1,4-beta-glucanase, GH124 endoglucanase, GH45 endoglucanase, gh45-1, GH5 cellulase, GH5 endoglucanase, GH6 endoglucanase, GH7 endoglucanase, GH9 termite cellulase, Glu1, Glu2, glycoside hydrolase family 9 endoglucanase, GtGH45, Lp-egl-1, manganese dependent endoglucanase, Maxazyme, Meicelase, mesophilic endoglucanase, mgCel6A, More, MtGH45, nmGH45, Onozuka R10, pancellase SS, PaPopCel1, PF0854, PH1171, PttCel9A, RCE1, RCE2, Roth 3056, RtGH124, Rucel5B, Sl-cel7, Sl-cel9C1, SnEG54, SoCel5, ssgluc, SSO1354, SSO1354 enzyme, SSO1354 protein, SSO1949, SSO2534, STCE1, Sumizyme, T12-GE40, TC Serva, TeEG-I, TeEgl5A, TfCel9A, ThEG, theme C glycoside hydrolase family 9 endo-beta-glucanase, Thermoactive cellulase, thermostable carboxymethyl cellulase, THITE_2110957, TM_1525, TM_1751, umcel5G, umcel9y-1, Vul_Cel5A
ECTree
Posttranslational Modification
Posttranslational Modification on EC 3.2.1.4 - cellulase
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glycoprotein
-
-
glycoprotein
-
endoglucanase I contains 11% carbohydrate
glycoprotein
carbohydrate moieties are not necessary for enzymatic activity
glycoprotein
the enzyme has two potential N-glycosylation sites at the N-terminus (positions 37 and 60) and 60 potential O-glycosylation sites at the C-terminus
glycoprotein
-
the enzyme has two potential N-glycosylation sites at the N-terminus (positions 37 and 60) and 60 potential O-glycosylation sites at the C-terminus
-
glycoprotein
-
contains 8.9% carbohydrate
glycoprotein
-
contains 8.9% carbohydrate
-
glycoprotein
-
the enzyme contains 0.72% neutral sugar
glycoprotein
-
the enzyme contains 0.72% neutral sugar
-
glycoprotein
-
endoglucanase A, B and C are glycoproteins
glycoprotein
-
protein sequence has three putative N-glycosylation sites at residues 56-58, 99-101, and 237-239, respectively. Gylcosylated protein shows a molecular mass of 33 kDa compared with 28 kDa for ungycosylated form
glycoprotein
MW detected by SDS-PAGE is 130000 Da, after removal of N-glycans the MW is 60000 Da
glycoprotein
-
contains 7% carbohydrate
glycoprotein
-
cellulase A and B contain 5% carbohydrate
glycoprotein
-
total hexose content is 0.044 mg/mg protein
glycoprotein
-
cellulase I contains about 26% carbohydrate
glycoprotein
-
contains 39% carbohydrate
glycoprotein
-
contains 12.2% carbohydrate, the major sugar constituents are glucose and mannose
glycoprotein
treatment with endoglycosidase H decreases the molecular mass by about 2-5 kDa
glycoprotein
-
treatment with endoglycosidase H decreases the molecular mass by about 2-5 kDa
-
glycoprotein
-
53% carbohydrate content, molecular mass of the Endo H-treated enzyme is approximately 42 kDa
glycoprotein
-
93% carbohydrate content
glycoprotein
-
endoglucanase I contains 29.4% carbohydrate, endoglucanase II contains 5.3% carbohydrate, endoglucanase III and IV contain 6.4% carbohydrate
glycoprotein
-
membrane-bound cellulase
glycoprotein
-
glycoprotein
glycoprotein
-
galactose and glucose are the major constituent sugars. Glucosamine is detected in both cellulase A and B. Galactosamine is detected in cellulase C
glycoprotein
enzyme migrates on SDS-PAGE with several bands, with molecular masses larger than its theoretical value. after Endo H-treatment, Egl5A migrates as a single band of 37000 Da
glycoprotein
-
carbohydrate content: endocellulases I (27.7%), endocellulases II (29.9%), endocellulases III (44.7%), endocellulases IV (50.8%)
glycoprotein
x * 51475, MALDI-TOF of recombinant protein, x * 47206, MALDI-TOF of recombinant deglycosylated protein
glycoprotein
treatment with endo-beta-N-acetylglucosaminidase H (endo H) to remove glycosylation
glycoprotein
-
enzyme migrates on SDS-PAGE with several bands, with molecular masses larger than its theoretical value. after Endo H-treatment, Egl5A migrates as a single band of 37000 Da
-
glycoprotein
-
contains 10.5% carbohydrate
glycoprotein
-
contains 10.5% carbohydrate
-
glycoprotein
enzyme is highly glycosylated
glycoprotein
-
enzyme is highly glycosylated
-
glycoprotein
-
recombinant protein produced in Pichia pastoris has a molecular mass of 47 kDa, while native protein from Sclerotinia sclerotiorum has a mass of 34 kDa
glycoprotein
Sporotrichum pulverulentum
-
-
glycoprotein
Sporotrichum pulverulentum
-
isoenzyme T1 contains 10.5% carbohydrate, isoenzyme T2b contains 7.8% carbohydrate, isoenzyme T3a contains 4.7% carbohydrate, isoenzyme T3b contains 2.2% carbohydrate
glycoprotein
three N-linked glycosylation sites (Asn23, Asn64, and Asn76) are possible, based on protein sequence and structure. Treatment with endo-beta-N-acetylglucosaminidase H (endo H) to remove glycosylation
glycoprotein
-
glycoprotein
glycoprotein
-
cellulase I contains 5.5% carbohydrate, cellulase II contains 2.6% carbohydrate, cellulase III contains 1.8% carbohydrate
glycoprotein
-
cellulase I contains 5.5% carbohydrate. Cellulase II conatins 2.6% carbohydrate. Cellulase III contains 1.8% carbohydrate
glycoprotein
-
enzyme E2 and E3 are glycoproteins
glycoprotein
-
endoglucanase E2 contains 25% carbohydrate
glycoprotein
Thermochaetoides thermophila
-
-
glycoprotein
Thermochaetoides thermophila
-
glycoprotein
Thermochaetoides thermophila
-
contains 18.9% carbohydrate
glycoprotein
Thermochaetoides thermophila
-
carbohydrate content 17%, isoform cellobiohydrolase I, 22.8%, isoform cellobiohydrolase I, respectively
glycoprotein
Thermochaetoides thermophila
-
carbohydrate content 18.9%
glycoprotein
Thermochaetoides thermophila
recombinant protein expressed in Pichia pastoris
glycoprotein
Thermochaetoides thermophila
predicted one N-linked glycosylation site (N261) and three O-linked glycosylation sites (T32, T312 and T332), respectively
glycoprotein
Thermochaetoides thermophila CBS 144.50
-
-
-
glycoprotein
Thermochaetoides thermophila CBS 144.50
-
predicted one N-linked glycosylation site (N261) and three O-linked glycosylation sites (T32, T312 and T332), respectively
-
glycoprotein
Thermochaetoides thermophila CT2
-
carbohydrate content 18.9%
-
glycoprotein
Thermochaetoides thermophila CT2
-
contains 18.9% carbohydrate
-
glycoprotein
Thermochaetoides thermophila DSM 1495
-
-
-
glycoprotein
Thermochaetoides thermophila DSM 1495
-
predicted one N-linked glycosylation site (N261) and three O-linked glycosylation sites (T32, T312 and T332), respectively
-
glycoprotein
Thermochaetoides thermophila IMI 039719
-
-
-
glycoprotein
Thermochaetoides thermophila IMI 039719
-
predicted one N-linked glycosylation site (N261) and three O-linked glycosylation sites (T32, T312 and T332), respectively
-
glycoprotein
sparsely glycosylated
glycoprotein
-
different extents of asparagine-linked glycosylation. With increased glycosylation extent of the enzyme components: C2, C1 and native Cel5A, the pH range of activity becomes wider, and thermal stability becomes higher
glycoprotein
removal of one or two N-glycosylation sites hardly affects the extracellular secretion of CBH1. Elimination of all of the glycosylation sites induces expression of the unfolded protein response target genes, and secretion of this CBH1 variant is severely compromised in a calnexin gene deletion strain. The thermal reactivity of CBH1 is significantly decreased by removal of either Asn45 or Asn384 glycosylation site during the catalyzed hydrolysis of soluble substrate. Combinatorial loss of these two N-linked glycans further exacerbates the temperature-dependent inactivation. Removal of N-glycosylation at Asn384 has a more pronounced effect on the integrity of regular secondary structure compared to the loss of Asn45 or Asn270
glycoprotein
-
high-molecular weight enzyme form and low-molecular weight enzyme form contain 21% carbohydrate
glycoprotein
-
endoglucanases I, II, III, V, and VI are glycoproteins
glycoprotein
-
cellulase II-A contains 12% carbohydrate, cellulase II-B contains 14% carbohydrate
glycoprotein
-
endoglucanases I, II, III, V, and VI are glycoproteins
-
no glycoprotein
-
no glycoprotein
lack of glycosylation is determined using PNGase F
no glycoprotein
-
lack of glycosylation is determined using PNGase F
-
no modification
-
-
no modification
-
contains no carbohydrate
no modification
-
contains no carbohydrate
no modification
-
contains no carbohydrate
no modification
-
contains no carbohydrate
no modification
-
the 9.5 cellulase is not a glycoprotein
no modification
-
contains less than 1% carbohydrate
no modification
-
contains no carbohydrate
no modification
-
contains no carbohydrate
-
no modification
-
cellulase I and II are not glycoproteins
no modification
Sporotrichum pulverulentum
-
-
no modification
Sporotrichum pulverulentum
-
isoenzyme T2a contains no carbohydrate
no modification
-
endoglucanase E1 may not be a glycoprotein
no modification
-
enzyme E1, E4 and E5 are not glycoproteins
no modification
-
endoglucanase IV contains no carbohydrate
no modification
-
endoglucanase IV contains no carbohydrate
-
proteolytic modification
sequence contains signal peptide-like amino acids
proteolytic modification
Actinomyces sp. 40 Korean Native Goat 40
-
sequence contains signal peptide-like amino acids
-
proteolytic modification
sequence contains a putative signal peptide at residues 1-20
proteolytic modification
-
sequence contains a putative signal peptide at residues 1-20
-
proteolytic modification
sequence contains a signal peptide of 21 amino acids
additional information
sequence contains a carbohydrate binding module family 1 at the C-terminus, and one potential N-glycosylation site (Asn-Thr-Ser) is found
additional information
-
sequence contains a carbohydrate binding module family 1 at the C-terminus, and one potential N-glycosylation site (Asn-Thr-Ser) is found
additional information
the N-terminal glutamine undergoes a cyclization and condensation reaction with the amino group of the N-terminus, to produce a cyclic diglutamate
additional information
-
the N-terminal glutamine undergoes a cyclization and condensation reaction with the amino group of the N-terminus, to produce a cyclic diglutamate