Any feedback?
Literature summary for 3.2.1.4 extracted from
- Structural and biochemical studies of GH family 12 cellulases: improved thermal stability, and ligand complexes (2005), Prog. Biophys. Mol. Biol., 89, 246-291.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| exprression in Aspergillus niger | Trichoderma reesei |
| exprression in Aspergillus niger | Trichoderma citrinoviride |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Trichoderma reesei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G170C | Tm is 2.1°C higher than wild-type value, specific activity is 1.2fold higher than wild-type enzyme | Trichoderma reesei |
| G170C/P201C | Tm is 0.7°C higher than wild-type value, specific activity is 38% of wild-type value | Trichoderma reesei |
| G170C/P201C/V210C | Tm is 3.9°C higher than wild-type value, specific activity is 14% of wild-type value | Trichoderma reesei |
| G170C/V210C | specific activity is 1.4fold higher than wild-type value | Trichoderma reesei |
| P201C | Tm is 0.7°C higher than wild-type value, specific activity is 50% of wild-type value | Trichoderma reesei |
| P201C | Tm is 3.9°C higher than wild-type value, specific activity is 80% of wild-type value | Trichoderma reesei |
| V210C | Tm is 0.1°C higher than wild-type value, specific activity is 1.8fold of the wild-type value | Trichoderma reesei |
| V210C | Tm is identical to wild-type value, specific activity is 80% of wild-type value | Trichoderma reesei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
- |
Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clonostachys rosea | - |
- |
- |
| Fusarium solani-melongenae | - |
- |
- |
| Trichocladium griseum | - |
- |
- |
| Trichoderma citrinoviride | - |
- |
- |
| Trichoderma koningii | - |
- |
- |
| Trichoderma reesei | O00095 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | sparsely glycosylated | Trichoderma reesei |
| additional information | the N-terminal glutamine undergoes a cyclization and condensation reaction with the amino group of the N-terminus, to produce a cyclic diglutamate | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | - |
Clonostachys rosea | ? | - |
? |  |
| cellulose + H2O | - |
Trichoderma koningii | ? | - |
? | |
| cellulose + H2O | - |
Trichocladium griseum | ? | - |
? | |
| cellulose + H2O | - |
Fusarium solani-melongenae | ? | - |
? | |
| cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
| cellulose + H2O | - |
Trichoderma citrinoviride | ? | - |
? | |
| o-nitrophenyl beta-cellobioside + H2O | - |
Clonostachys rosea | ? | - |
? | |
| o-nitrophenyl beta-cellobioside + H2O | - |
Trichoderma koningii | ? | - |
? | |
| o-nitrophenyl beta-cellobioside + H2O | - |
Trichocladium griseum | ? | - |
? | |
| o-nitrophenyl beta-cellobioside + H2O | - |
Fusarium solani-melongenae | ? | - |
? | |
| o-nitrophenyl beta-cellobioside + H2O | - |
Trichoderma reesei | ? | - |
? | |
| o-nitrophenyl beta-cellobioside + H2O | - |
Trichoderma citrinoviride | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel12A | - |
Clonostachys rosea |
| Cel12A | - |
Trichoderma koningii |
| Cel12A | - |
Trichocladium griseum |
| Cel12A | - |
Fusarium solani-melongenae |
| Cel12A | - |
Trichoderma reesei |
| Cel12A | - |
Trichoderma citrinoviride |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
Tm: 45.9°C | Clonostachys rosea |
| 49 | - |
Tm: 49.2°C | Trichoderma citrinoviride |
| 54 | - |
Tm: 54.1°C | Fusarium solani-melongenae |
| 54 | - |
Tm: 54.4°C | Trichoderma reesei |
| 58.5 | - |
Tm-value | Trichoderma koningii |
| 67 | - |
Tm: 66.8°C | Trichoderma citrinoviride |
| 69 | - |
Tm: 68.6°C | Trichocladium griseum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Trichoderma koningii |
| 5.5 | - |
- |
Trichocladium griseum |
| 5.5 | - |
- |
Trichoderma citrinoviride |
| 5.5 | - |
substrate: o-nitrophenyl beta-cellobioside | Trichoderma reesei |
| 8 | - |
- |
Trichoderma citrinoviride |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
optimal stability | Clonostachys rosea |
| 5 | - |
optimal stability | Trichoderma koningii |
| 5 | - |
optimal stability | Fusarium solani-melongenae |
| 5 | - |
optimal stability | Trichoderma reesei |
| 5 | - |
optimal stability | Trichoderma citrinoviride |
| 7 | - |
optimal stability | Trichocladium griseum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trichoderma reesei | - |
- |
7.5 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
