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Literature summary for 3.2.1.4 extracted from

  • Robson, L.M.; Chambliss, G.H.
    Cellulases of bacterial origin (1989), Enzyme Microb. Technol., 11, 626-644.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
endoglucanase Y Dickeya chrysanthemi
expression in Escherichia coli Ruminococcus albus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slight inhibition of endoglucanase E2 activity Thermobifida fusca
cellobiose
-
Acetivibrio cellulolyticus
cellobiose
-
Acetivibrio thermocellus
cellobiose 80 mM, 14% loss of activity, endoglucanase Z Dickeya chrysanthemi
cellobiose
-
Thermobifida fusca
glucose 162 mM, 23% loss of activity, endoglucanase Z Dickeya chrysanthemi
Mg2+ slight inhibition of endoglucanase E2 activity Thermobifida fusca

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular endoglucanase C2 and C3 Acetivibrio cellulolyticus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Acetivibrio thermocellus
Ca2+
-
Acetivibrio cellulolyticus
Ca2+ stimulates activity of endoglucanase E1 Thermobifida fusca
Mg2+
-
Acetivibrio thermocellus
Mg2+
-
Acetivibrio cellulolyticus
Mg2+ stimulates activity of endoglucanase E1 Thermobifida fusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, endoglucanase Y, SDS-PAGE Dickeya chrysanthemi
40000
-
x * 40000, enzyme form EGB, SDS-PAGE Acetivibrio thermocellus
45000
-
endoglucanase Z Dickeya chrysanthemi
56000
-
x * 56000, enzyme form EGA, SDS-PAGE Acetivibrio thermocellus
65000
-
x * 65000, enzyme form EGD, SDS-PAGE Acetivibrio thermocellus
66000
-
x * 66000, enzyme form EGB, SDS-PAGE Acetivibrio thermocellus
94000
-
1 * 94000, endoglucanase E1, SDS-PAGE Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Acetivibrio cellulolyticus
-
-
-
Acetivibrio thermocellus
-
enzyme form EGA, EGB, EGC and EGD
-
Bacillus sp. (in: Bacteria)
-
No. 1139
-
Bacillus sp. (in: Bacteria)
-
N-4
-
Bacillus sp. (in: Bacteria) N-4
-
N-4
-
Bacillus sp. (in: Bacteria) No. 1139
-
No. 1139
-
Bacillus subtilis
-
DLG, N-24, or PAP115
-
Cellulomonas fimi
-
endoglucanase A and B
-
Dickeya chrysanthemi
-
endoglucanase Z and Y
-
Ruminococcus albus
-
F40
-
Ruminococcus albus F40
-
F40
-
Thermobifida fusca
-
endoglucanase E1, E2, and E5
-
Thermomonospora curvata
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Acetivibrio thermocellus
glycoprotein endoglucanase E2 contains 25% carbohydrate Thermobifida fusca
no modification
-
Acetivibrio thermocellus
no modification endoglucanase E1 may not be a glycoprotein Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Bacillus subtilis additional information
-
?
carboxymethylcellulose + H2O
-
Bacillus sp. (in: Bacteria) additional information
-
?
carboxymethylcellulose + H2O
-
Ruminococcus albus additional information
-
?
carboxymethylcellulose + H2O
-
Dickeya chrysanthemi additional information
-
?
carboxymethylcellulose + H2O
-
Acetivibrio thermocellus additional information
-
?
carboxymethylcellulose + H2O
-
Cellulomonas fimi additional information
-
?
carboxymethylcellulose + H2O
-
Acetivibrio cellulolyticus additional information
-
?
carboxymethylcellulose + H2O endoglucanase E1 exhibits higher activity Thermobifida fusca additional information endoglucanase E2 produces glucose, cellobiose, cellotriose and higher oligosaccharides. The major end product produced by endoglucanase E1 is cellobiose and small amounts of glucose ?
carboxymethylcellulose + H2O
-
Bacillus sp. (in: Bacteria) No. 1139 additional information
-
?
carboxymethylcellulose + H2O
-
Bacillus sp. (in: Bacteria) N-4 additional information
-
?
carboxymethylcellulose + H2O
-
Ruminococcus albus F40 additional information
-
?
cellopentaose + H2O
-
Ruminococcus albus additional information
-
?
cellopentaose + H2O enzyme form EGB and EGC Acetivibrio thermocellus additional information
-
?
cellopentaose + H2O
-
Ruminococcus albus F40 additional information
-
?
cellotetraose + H2O
-
Bacillus sp. (in: Bacteria) 2 cellobiose
-
?
cellotetraose + H2O
-
Ruminococcus albus 2 cellobiose
-
?
cellotetraose + H2O effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2 Thermobifida fusca 2 cellobiose
-
?
cellotetraose + H2O enzyme form EGB and EGC Acetivibrio thermocellus 2 cellobiose
-
?
cellotetraose + H2O
-
Bacillus sp. (in: Bacteria) No. 1139 2 cellobiose
-
?
cellotetraose + H2O
-
Bacillus sp. (in: Bacteria) N-4 2 cellobiose
-
?
cellotetraose + H2O
-
Ruminococcus albus F40 2 cellobiose
-
?
cellotriose + H2O
-
Bacillus sp. (in: Bacteria) D-glucose + cellobiose
-
?
cellotriose + H2O enzyme form EGC Acetivibrio thermocellus D-glucose + cellobiose
-
?
cellotriose + H2O effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2 Thermobifida fusca D-glucose + cellobiose
-
?
cellotriose + H2O
-
Bacillus sp. (in: Bacteria) No. 1139 D-glucose + cellobiose
-
?
cellotriose + H2O
-
Bacillus sp. (in: Bacteria) N-4 D-glucose + cellobiose
-
?
cellulose + H2O
-
Acetivibrio cellulolyticus ?
-
?
cellulose + H2O endoglucanase E1 exhibits higher activity Thermobifida fusca ?
-
?
cellulose + H2O acid-swollen Ruminococcus albus ?
-
?
cellulose + H2O acid-swollen Thermobifida fusca ?
-
?
cellulose + H2O acid-swollen Acetivibrio thermocellus ?
-
?
cellulose + H2O acid-swollen Dickeya chrysanthemi ? major end product are cellobiose and cellotriose, endoglucanase Z ?
cellulose + H2O enzyme form EGA and EGD Acetivibrio thermocellus ?
-
?
cellulose + H2O acid-swollen Ruminococcus albus F40 ?
-
?
lichenan + H2O enzyme form EGD Acetivibrio thermocellus ?
-
?
lichenan + H2O endoglucanase A Cellulomonas fimi ?
-
?
xylan + H2O
-
Thermobifida fusca additional information
-
?
xylan + H2O endoglucanase B Cellulomonas fimi additional information
-
?

Subunits

Subunits Comment Organism
? x * 56000, enzyme form EGA, SDS-PAGE Acetivibrio thermocellus
? 1 * 94000, endoglucanase E1, SDS-PAGE Thermobifida fusca
? x * 40000, enzyme form EGB, SDS-PAGE Acetivibrio thermocellus
? x * 66000, enzyme form EGB, SDS-PAGE Acetivibrio thermocellus
? x * 35000, endoglucanase Y, SDS-PAGE Dickeya chrysanthemi
? x * 65000, enzyme form EGD, SDS-PAGE Acetivibrio thermocellus
More enzyme form C2, 33000 Da, is likely to by a trimer of endoglucanase C3, 104000 Da Acetivibrio cellulolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
hydrolysis of carboxymethylcellulose, endoglucanase Z Dickeya chrysanthemi
58
-
endoglucanase E2 Thermobifida fusca
60
-
-
Bacillus subtilis
60
-
enzyme form EGD Acetivibrio thermocellus
74
-
endoglucanase E1 Thermobifida fusca
75
-
enzyme form EGA Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)
50
-
stable up to Bacillus subtilis
60
-
-
Bacillus sp. (in: Bacteria)
60
-
enzyme form EGA, stable up to Acetivibrio thermocellus
65
-
-
Bacillus subtilis
65
-
enzyme form EGC, stable up to Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
Bacillus subtilis
5.5
-
-
Bacillus subtilis
5.5
-
endoglucanase Y Dickeya chrysanthemi
5.5 6.5 enzyme form EGA Acetivibrio thermocellus
6
-
endoglucanase E1 and E2 Thermobifida fusca
6
-
enzyme form EGC and EGD Acetivibrio thermocellus
6.2 7.5 hydrolysis of carboxymethylcellulose, endoglucanase Z Dickeya chrysanthemi