Cloned (Comment) | Organism |
---|---|
endoglucanase Y | Dickeya chrysanthemi |
expression in Escherichia coli | Ruminococcus albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight inhibition of endoglucanase E2 activity | Thermobifida fusca | |
cellobiose | - |
Acetivibrio cellulolyticus | |
cellobiose | - |
Acetivibrio thermocellus | |
cellobiose | 80 mM, 14% loss of activity, endoglucanase Z | Dickeya chrysanthemi | |
cellobiose | - |
Thermobifida fusca | |
glucose | 162 mM, 23% loss of activity, endoglucanase Z | Dickeya chrysanthemi | |
Mg2+ | slight inhibition of endoglucanase E2 activity | Thermobifida fusca |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | endoglucanase C2 and C3 | Acetivibrio cellulolyticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Acetivibrio thermocellus | |
Ca2+ | - |
Acetivibrio cellulolyticus | |
Ca2+ | stimulates activity of endoglucanase E1 | Thermobifida fusca | |
Mg2+ | - |
Acetivibrio thermocellus | |
Mg2+ | - |
Acetivibrio cellulolyticus | |
Mg2+ | stimulates activity of endoglucanase E1 | Thermobifida fusca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, endoglucanase Y, SDS-PAGE | Dickeya chrysanthemi |
40000 | - |
x * 40000, enzyme form EGB, SDS-PAGE | Acetivibrio thermocellus |
45000 | - |
endoglucanase Z | Dickeya chrysanthemi |
56000 | - |
x * 56000, enzyme form EGA, SDS-PAGE | Acetivibrio thermocellus |
65000 | - |
x * 65000, enzyme form EGD, SDS-PAGE | Acetivibrio thermocellus |
66000 | - |
x * 66000, enzyme form EGB, SDS-PAGE | Acetivibrio thermocellus |
94000 | - |
1 * 94000, endoglucanase E1, SDS-PAGE | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio cellulolyticus | - |
- |
- |
Acetivibrio thermocellus | - |
enzyme form EGA, EGB, EGC and EGD | - |
Bacillus sp. (in: Bacteria) | - |
No. 1139 | - |
Bacillus sp. (in: Bacteria) | - |
N-4 | - |
Bacillus sp. (in: Bacteria) N-4 | - |
N-4 | - |
Bacillus sp. (in: Bacteria) No. 1139 | - |
No. 1139 | - |
Bacillus subtilis | - |
DLG, N-24, or PAP115 | - |
Cellulomonas fimi | - |
endoglucanase A and B | - |
Dickeya chrysanthemi | - |
endoglucanase Z and Y | - |
Ruminococcus albus | - |
F40 | - |
Ruminococcus albus F40 | - |
F40 | - |
Thermobifida fusca | - |
endoglucanase E1, E2, and E5 | - |
Thermomonospora curvata | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Acetivibrio thermocellus |
glycoprotein | endoglucanase E2 contains 25% carbohydrate | Thermobifida fusca |
no modification | - |
Acetivibrio thermocellus |
no modification | endoglucanase E1 may not be a glycoprotein | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Bacillus subtilis | additional information | - |
? | |
carboxymethylcellulose + H2O | - |
Bacillus sp. (in: Bacteria) | additional information | - |
? | |
carboxymethylcellulose + H2O | - |
Ruminococcus albus | additional information | - |
? | |
carboxymethylcellulose + H2O | - |
Dickeya chrysanthemi | additional information | - |
? | |
carboxymethylcellulose + H2O | - |
Acetivibrio thermocellus | additional information | - |
? | |
carboxymethylcellulose + H2O | - |
Cellulomonas fimi | additional information | - |
? | |
carboxymethylcellulose + H2O | - |
Acetivibrio cellulolyticus | additional information | - |
? | |
carboxymethylcellulose + H2O | endoglucanase E1 exhibits higher activity | Thermobifida fusca | additional information | endoglucanase E2 produces glucose, cellobiose, cellotriose and higher oligosaccharides. The major end product produced by endoglucanase E1 is cellobiose and small amounts of glucose | ? | |
carboxymethylcellulose + H2O | - |
Bacillus sp. (in: Bacteria) No. 1139 | additional information | - |
? | |
carboxymethylcellulose + H2O | - |
Bacillus sp. (in: Bacteria) N-4 | additional information | - |
? | |
carboxymethylcellulose + H2O | - |
Ruminococcus albus F40 | additional information | - |
? | |
cellopentaose + H2O | - |
Ruminococcus albus | additional information | - |
? | |
cellopentaose + H2O | enzyme form EGB and EGC | Acetivibrio thermocellus | additional information | - |
? | |
cellopentaose + H2O | - |
Ruminococcus albus F40 | additional information | - |
? | |
cellotetraose + H2O | - |
Bacillus sp. (in: Bacteria) | 2 cellobiose | - |
? | |
cellotetraose + H2O | - |
Ruminococcus albus | 2 cellobiose | - |
? | |
cellotetraose + H2O | effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2 | Thermobifida fusca | 2 cellobiose | - |
? | |
cellotetraose + H2O | enzyme form EGB and EGC | Acetivibrio thermocellus | 2 cellobiose | - |
? | |
cellotetraose + H2O | - |
Bacillus sp. (in: Bacteria) No. 1139 | 2 cellobiose | - |
? | |
cellotetraose + H2O | - |
Bacillus sp. (in: Bacteria) N-4 | 2 cellobiose | - |
? | |
cellotetraose + H2O | - |
Ruminococcus albus F40 | 2 cellobiose | - |
? | |
cellotriose + H2O | - |
Bacillus sp. (in: Bacteria) | D-glucose + cellobiose | - |
? | |
cellotriose + H2O | enzyme form EGC | Acetivibrio thermocellus | D-glucose + cellobiose | - |
? | |
cellotriose + H2O | effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2 | Thermobifida fusca | D-glucose + cellobiose | - |
? | |
cellotriose + H2O | - |
Bacillus sp. (in: Bacteria) No. 1139 | D-glucose + cellobiose | - |
? | |
cellotriose + H2O | - |
Bacillus sp. (in: Bacteria) N-4 | D-glucose + cellobiose | - |
? | |
cellulose + H2O | - |
Acetivibrio cellulolyticus | ? | - |
? | |
cellulose + H2O | endoglucanase E1 exhibits higher activity | Thermobifida fusca | ? | - |
? | |
cellulose + H2O | acid-swollen | Ruminococcus albus | ? | - |
? | |
cellulose + H2O | acid-swollen | Thermobifida fusca | ? | - |
? | |
cellulose + H2O | acid-swollen | Acetivibrio thermocellus | ? | - |
? | |
cellulose + H2O | acid-swollen | Dickeya chrysanthemi | ? | major end product are cellobiose and cellotriose, endoglucanase Z | ? | |
cellulose + H2O | enzyme form EGA and EGD | Acetivibrio thermocellus | ? | - |
? | |
cellulose + H2O | acid-swollen | Ruminococcus albus F40 | ? | - |
? | |
lichenan + H2O | enzyme form EGD | Acetivibrio thermocellus | ? | - |
? | |
lichenan + H2O | endoglucanase A | Cellulomonas fimi | ? | - |
? | |
xylan + H2O | - |
Thermobifida fusca | additional information | - |
? | |
xylan + H2O | endoglucanase B | Cellulomonas fimi | additional information | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, enzyme form EGA, SDS-PAGE | Acetivibrio thermocellus |
? | 1 * 94000, endoglucanase E1, SDS-PAGE | Thermobifida fusca |
? | x * 40000, enzyme form EGB, SDS-PAGE | Acetivibrio thermocellus |
? | x * 66000, enzyme form EGB, SDS-PAGE | Acetivibrio thermocellus |
? | x * 35000, endoglucanase Y, SDS-PAGE | Dickeya chrysanthemi |
? | x * 65000, enzyme form EGD, SDS-PAGE | Acetivibrio thermocellus |
More | enzyme form C2, 33000 Da, is likely to by a trimer of endoglucanase C3, 104000 Da | Acetivibrio cellulolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
hydrolysis of carboxymethylcellulose, endoglucanase Z | Dickeya chrysanthemi |
58 | - |
endoglucanase E2 | Thermobifida fusca |
60 | - |
- |
Bacillus subtilis |
60 | - |
enzyme form EGD | Acetivibrio thermocellus |
74 | - |
endoglucanase E1 | Thermobifida fusca |
75 | - |
enzyme form EGA | Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
50 | - |
stable up to | Bacillus subtilis |
60 | - |
- |
Bacillus sp. (in: Bacteria) |
60 | - |
enzyme form EGA, stable up to | Acetivibrio thermocellus |
65 | - |
- |
Bacillus subtilis |
65 | - |
enzyme form EGC, stable up to | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Bacillus subtilis |
5.5 | - |
- |
Bacillus subtilis |
5.5 | - |
endoglucanase Y | Dickeya chrysanthemi |
5.5 | 6.5 | enzyme form EGA | Acetivibrio thermocellus |
6 | - |
endoglucanase E1 and E2 | Thermobifida fusca |
6 | - |
enzyme form EGC and EGD | Acetivibrio thermocellus |
6.2 | 7.5 | hydrolysis of carboxymethylcellulose, endoglucanase Z | Dickeya chrysanthemi |