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0.2038 - 0.2085
2',4'-dinitrophenyl-beta-D-cellobiose
0.197 - 0.697
2,4-dinitrophenyl beta-D-cellobioside
0.21 - 0.33
4-methylumbelliferyl beta-D-lactoside
0.1 - 0.125
4-methylumbelliferyl cellobioside
0.15 - 0.37
4-nitrophenyl cellobioside
0.36
4-nitrophenyl cellopentaoside
pH 6.0, 95°C
0.12
4-nitrophenyl cellotetraoside
pH 6.0, 95°C
0.061
4-nitrophenyl cellotrioside
pH 6.0, 95°C
0.352 - 1.4
4-nitrophenyl D-cellobioside
13.3
Barley beta-glucan
pH 7.0, 37°C
0.0037 - 20.8
carboxymethyl cellulose
-
0.15 - 7.24
cellopentaose
0.04 - 18.3
cellotetraose
1.116
filter paper
-
pH 5.5, 50°C
-
28.3
hydroxyethylcellulose
pH 7.0, 37°C
47.3
Laminarin
pH 7.0, 37°C
0.0017 - 0.0055
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
0.002
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
0.35 - 6.69
p-nitrophenyl cellobiose
1.5 - 4
p-nitrophenyl-beta-D-cellobioside
0.24
p-nitrophenyl-beta-D-cellopentaoside
-
0.25
p-nitrophenyl-beta-D-cellotetraoside
-
0.174
rice husk
-
pH 5.5, 50°C
-
0.092
Smallanthus sonchifolius cellulose
-
pH 5.5, 50°C
-
0.027
soybean husk
-
pH 5.5, 50°C
-
0.092
sugarcane bagasse
-
pH 5.5, 50°C
-
0.211
wheat straw
-
pH 5.5, 50°C
-
16.72
yam cellulose
-
pH 5.5, 50°C
-
additional information
Avicel
-
0.2038
2',4'-dinitrophenyl-beta-D-cellobiose
-
endoglucanase A
0.2085
2',4'-dinitrophenyl-beta-D-cellobiose
-
chimeric xylanase/endoglucanase
0.197
2,4-dinitrophenyl beta-D-cellobioside
50°C, mutant enzyme E424G
0.29
2,4-dinitrophenyl beta-D-cellobioside
50°C, mutant enzyme E424A
0.697
2,4-dinitrophenyl beta-D-cellobioside
50°C, wild-type enzyme Cel9A-68
0.21
4-methylumbelliferyl beta-D-lactoside
mutant N54C/P191C, pH 5.0, 22°C
0.23
4-methylumbelliferyl beta-D-lactoside
mutant G4C/A72C/N54C/P191C/T243C/A375C, pH 5.0, 22°C
0.26
4-methylumbelliferyl beta-D-lactoside
mutant G4C/A72C, pH 5.0, 22°C
0.31
4-methylumbelliferyl beta-D-lactoside
deglycosylated wild-type, pH 5.0, 22°C
0.31
4-methylumbelliferyl beta-D-lactoside
mutant T243C/A375C, pH 5.0, 22°C
0.33
4-methylumbelliferyl beta-D-lactoside
wild-type, pH 5.0, 22°C
0.1
4-methylumbelliferyl cellobioside
mutant Q274V, pH 5.0, 45°C
0.111
4-methylumbelliferyl cellobioside
mutant K272F, pH 5.0, 45°C
0.114
4-methylumbelliferyl cellobioside
mutant Q126F, pH 5.0, 45°C
0.125
4-methylumbelliferyl cellobioside
wild-type, pH 5.0, 45°C
0.15
4-nitrophenyl cellobioside
pH 6.0, 95°C
0.37
4-nitrophenyl cellobioside
pH 6.0, 70°C
0.352
4-nitrophenyl D-cellobioside
N-glycosylation mutant residue D270, pH 4.8, 45°C
0.363
4-nitrophenyl D-cellobioside
wild-type, pH 4.8, 45°C
0.369
4-nitrophenyl D-cellobioside
N-glycosylation mutant residue D45, pH 4.8, 45°C
0.426
4-nitrophenyl D-cellobioside
N-glycosylation mutant residue 384, pH 4.8, 45°C
1.4
4-nitrophenyl D-cellobioside
pH 7.0, 37°C
0.0037
carboxymethyl cellulose
pH 5.0, 50°C
-
0.0037
carboxymethyl cellulose
pH 5.0, 50°C
-
1.08
carboxymethyl cellulose
-
pH 5.0, 70°C
-
1.97
carboxymethyl cellulose
mutant E225H-K207G-D37V, pH 6.0, 85°C
-
2.97
carboxymethyl cellulose
mutant E225H-K207G, pH 6.0, 85°C
-
3.11
carboxymethyl cellulose
-
pH 5.0, 75°C
-
3.97
carboxymethyl cellulose
-
pH 5.0, 55°C
-
12.5
carboxymethyl cellulose
-
pH 6.0, 95°C
-
20.8
carboxymethyl cellulose
pH 7.0, 37°C
-
0.33
cellohexaose
-
cellulase II-A
5.1
cellohexaose
pH 7.0, 37°C
0.15
cellopentaose
-
cellulase II-A
0.28
cellopentaose
-
cellulase II-B
0.46
cellopentaose
-
cellulase II-A
0.62
cellopentaose
pH 7.0, 37°C
0.04
cellotetraose
-
cellulase II-A
0.42
cellotetraose
-
cellulase II-B
18.3
cellotetraose
pH 7.0, 37°C
1.63
cellotriose
-
cellulase II-B
151.7
cellotriose
pH 7.0, 37°C
0.0017
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH 3, 80°C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
0.0017
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH 3, 80°C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
0.00174
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH and temperature not specified in the publication, hybrid protein (CelA-SSO1949-CelA, consisting of 262 amino acids) based on beta-endoglucanase from Sulfolobus solfataricus P2 and beta-endoglucanase from Thermotoga maritima
0.0055
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH 3, 80°C, native enzyme
0.002
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH 1.8, 80°C
0.002
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH 1.8, 80°C
0.35
p-nitrophenyl cellobiose
50°C, mutant enzyme C372A/C412A
0.46
p-nitrophenyl cellobiose
50°C, mutant enzyme C106A/C159A
0.76
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme DELTAQ1-G5
0.78
p-nitrophenyl cellobiose
50°C, mutant enzyme C106A/C159A/C372A/C412A
0.88
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme I157A
0.92
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme T160A
0.95
p-nitrophenyl cellobiose
50°C, wild-type enzyme
0.95
p-nitrophenyl cellobiose
pH 5-6, 50°C, wild-type enzyme
1.12
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme Y299F
1.12
p-nitrophenyl cellobiose
pH 5.5, 50°C, wild-type enzyme
1.13
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus and 5 residies at the N-terminus
1.23
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme H161A
1.24
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme E201Q
1.37
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme G158A
1.44
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus
1.55
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme E163A
1.58
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme P164A
1.9
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme C159A
2.4
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme D385N
2.76
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme H297N
4.57
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme I162A
5.85
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme R156A
6.69
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme H155A
1.5
p-nitrophenyl-beta-D-cellobioside
-
4°C
4
p-nitrophenyl-beta-D-cellobioside
-
35°C
additional information
Avicel
-
Km value is 1.01 mg/ml, pH 6.0, 65°C
-
additional information
Avicel
Km-value 19.5 g/l, pH 5.0, 50°C
-
additional information
Avicel
Km value 0.0122 mg/ml, pH 5.0, 60°C
-
additional information
Avicel
-
Km value 0.0122 mg/ml, pH 5.0, 60°C
-
additional information
Barley beta-glucan
Km value 12.2 mg/l, pH 4.5, 90°C
additional information
Barley beta-glucan
Km-value 1.29 mg/ml, pH 6.5, 70°C
additional information
Barley beta-glucan
Km value 2.2 mg/ml, pH 6.5, 50°C
additional information
Barley beta-glucan
-
Km value 20 mg/ml for wild-type, 13 mg/ml for mutant N19A, 21 mg/ml for mutant N42A, 22 mg/ml for mutant N194A, respectively, pH 5.0, 40°C
additional information
beechwood xylan
Km-value: 60.6 mg/ml, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, pH 5.0, 70°C
-
additional information
beta-1,4-D-glucan
Thermochaetoides thermophila
Km-value: 9.82 mg/ml, pH 5.0, 55°C
additional information
beta-1,4-D-xylan
Thermochaetoides thermophila
Km-value: 10.28 mg/ml, pH 5.0, 55°C
additional information
beta-Glucan
Km value for wild-type is 2.08 mg/ml, for mutant Y61G 3.75 mg/ml, pH 5.0, 85°C
additional information
beta-Glucan
-
Km value for wild-type is 2.08 mg/ml, for mutant Y61G 3.75 mg/ml, pH 5.0, 85°C
additional information
beta-Glucan
Km value 22.8 mg/ml, pH 5.0, 50°C
additional information
beta-Glucan
-
Km value 22.8 mg/ml, pH 5.0, 50°C
additional information
birchwood xylan
Km value 4.37 mg/ml, pH 5.0, temperature not specified in the publication
-
additional information
carboxymethyl cellulose
-
KM value is 0.01 mg/ml, pH 4.8, 50°C
-
additional information
carboxymethyl cellulose
-
Km value is 0.13 mg/ml, pH not specified in the publication, temperature not specified in the publication
-
additional information
carboxymethyl cellulose
-
KM value is 0.20 mg/ml according to Lineweaver-Burk method and 0.28 mg/ml according to Hanes-Wolf method, pH 4.8, 50°C
-
additional information
carboxymethyl cellulose
-
Km value is 1.01 mg/ml, pH 6.0, 65°C
-
additional information
carboxymethyl cellulose
-
Km value is 2.1 mg/ml, pH 6.0, 50°C
-
additional information
carboxymethyl cellulose
Km value is 6.6 mg/ml in absence, 3.18 mg/ml in presence of 2.5 M NaCl, respectively, at pH 5.0, 55°C
-
additional information
carboxymethyl cellulose
-
Km value is 6.6 mg/ml in absence, 3.18 mg/ml in presence of 2.5 M NaCl, respectively, at pH 5.0, 55°C
-
additional information
carboxymethyl cellulose
-
Km value 1.9 mg/ml, pH 7.0, 65°C
-
additional information
carboxymethyl cellulose
Km value 31.7 mg/l, pH 4.5, 90°C
-
additional information
carboxymethyl cellulose
-
Km-value 0.00176 mM, pH 9.0, 40°C
-
additional information
carboxymethyl cellulose
Km-value 25.6 mg/ml, pH 5.5, 70°C
-
additional information
carboxymethyl cellulose
Km-value 8.3 g/l, pH 5.0, 50°C
-
additional information
carboxymethyl cellulose
Km-value is 2.35 mg/ml, pH 5.5, 92°C
-
additional information
carboxymethyl cellulose
Km-value is 2.5 mg/ml, pH 3.5, 70°C
-
additional information
carboxymethyl cellulose
Km-values for wild-type, mutants K94R, S365P and K94R/S365P are 7.2, 3.7, 6.4 and 5.0 mg/ml, respectively, pH 7.0, temperature not specified in the publication
-
additional information
carboxymethyl cellulose
-
Km-values for wild-type, mutants K94R, S365P and K94R/S365P are 7.2, 3.7, 6.4 and 5.0 mg/ml, respectively, pH 7.0, temperature not specified in the publication
-
additional information
carboxymethyl cellulose
46.2 mg/l and vmax: 17.1 micromol/min/mg, pH 7, 60°C
-
additional information
carboxymethyl cellulose
46.2 mg/l and vmax: 17.1 micromol/min/mg, pH 7, 60°C
-
additional information
carboxymethyl cellulose
71.9 mg/l and vmax: 28.6 micromol/min/mg, pH 7, 65°C
-
additional information
carboxymethyl cellulose
71.9 mg/l and vmax: 28.6 micromol/min/mg, pH 7, 65°C
-
additional information
carboxymethyl cellulose
-
Km value 37 mg/ml, pH 4.6, 60°C
-
additional information
carboxymethyl cellulose
Thermochaetoides thermophila
-
Km value 4.6 mg/ml, pH 5.0, 60°C
-
additional information
carboxymethyl cellulose
-
Km value in absence of Mn2+ 10.5 mg/ml, in presence of Mn2+ 7.8 mg/ml, pH 5.5, 60°C
-
additional information
carboxymethyl cellulose
Km-value: 6.8 mg/ml, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, pH 5.0, 70°C
-
additional information
carboxymethyl cellulose
Thermochaetoides thermophila
Km-value: 79.19 mg/ml, pH 5.0, 55°C
-
additional information
carboxymethylcellulose
-
Km of Egl330 and Egl499 are 1.73 x 10 mg/ml and 1.14 x 10 mg/ml, respectively. Vmax of Egl330 is 1.78fold higher than that of Egl499, increases from 284 U/mg to 786 U/mg. The catalytic efficiency (kcat/Km) of Egl330 is slightly higher than that of Egl499
-
additional information
carboxymethylcellulose
Thermochaetoides thermophila
-
1.67 mg/ml, pH 5.6, 50°C
-
additional information
carboxymethylcellulose
-
1.9 mg/ml, cellulase III, pH 4.5, 70°C
-
additional information
carboxymethylcellulose
-
3 mg/ml
-
additional information
carboxymethylcellulose
-
3.9 mg/ml, cellulase I, pH 4.5, 70°C
-
additional information
carboxymethylcellulose
Km of wild-type 7.75 mg/ml, of mutant lacking the IgG-like domain 7.24 mg/ml, respectively, pH 7.5, 65°C
-
additional information
carboxymethylcellulose
Km value 0.014 mg/ml, pH 5.0, 60°C
-
additional information
carboxymethylcellulose
-
Km value 0.014 mg/ml, pH 5.0, 60°C
-
additional information
carboxymethylcellulose
Thermochaetoides thermophila
Km value 0.060 mg/ml, pH 4.0, 70°C
-
additional information
carboxymethylcellulose
Km value 0.144 mg/ml, pH 5.0, temperature not specified in the publication
-
additional information
carboxymethylcellulose
Km value 1.83 mg/ml, pH 5.4, 70°C
-
additional information
carboxymethylcellulose
Km value 2 mg/mlpH 4.0, 70°C
-
additional information
carboxymethylcellulose
-
Km value 26.1 mg/ml, pH 5.0, 40°C
-
additional information
carboxymethylcellulose
Km value 4.5 mg/ml, pH 7.0, 45°C
-
additional information
carboxymethylcellulose
-
Km value 5 mg/ml, pH 10, 50°C
-
additional information
carboxymethylcellulose
Km value 6.72 mg/ml, pH 6.0, 37°C
-
additional information
carboxymethylcellulose
Km value 9.2 mg/ml for full-length protein, 11.5 mG/ml for catalytic domain, respectively, pH 7.0, 45°C
-
additional information
carboxymethylcellulose
Km value is 280.31 mg/ml, 222.74 mg/ml, and 110.22 mg/ml for wild-type and mutants V9A/K353E and G117S, respectively, pH 7.0, 40°C
-
additional information
carboxymethylcellulose
-
Km value is 280.31 mg/ml, 222.74 mg/ml, and 110.22 mg/ml for wild-type and mutants V9A/K353E and G117S, respectively, pH 7.0, 40°C
-
additional information
cellobiose
Km value 0.025 mg/ml, pH 5.0, 60°C
additional information
cellobiose
-
Km value 0.025 mg/ml, pH 5.0, 60°C
additional information
cellulose
13 celluloses characterized for crystallinity and crystal width (by X-ray diffraction), wet porosity (by thermoporometry), and particle size (by light scattering)
additional information
cellulose
-
Km-value for carboxymethylcellulose-Na as substrate is 3.01 mg/ml
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
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additional information
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-
-
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additional information
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-
-
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additional information
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-
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additional information
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-
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additional information
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-
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additional information
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-
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additional information
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-
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additional information
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-
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additional information
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-
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-
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additional information
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additional information
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-
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additional information
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-
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additional information
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-
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additional information
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-
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additional information
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-
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additional information
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additional information
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additional information
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-
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additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
Lenzites trabea
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
Bacillus cellulyticus K-12
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
immobilized enzyme
-
additional information
additional information
kinetic analysis
-
additional information
additional information
kinetic analysis
-
additional information
additional information
-
kinetic analysis
-
additional information
additional information
-
KM-value for carboxymethylcellulose is 1.5 mg/ml
-
additional information
additional information
-
KM-value for carboxymethylcellulose is 1.7 mg/ml
-
additional information
additional information
-
Km-value for carboxyymethylcellulose is 7.1 g/L at pH 8.5 and at 40°C
-
additional information
additional information
Thermochaetoides thermophila
-
the KM-value for the carboxymethylcellulose Na salt is 4.6 mg/ml
-
additional information
additional information
-
the Km-values for barley beta-glucan and carboxymethylcellulose are 0.32 mg/ml and 0.5 mg/ml
-
additional information
additional information
-
Km for carboxymethyl cellulose is 20 mg/ml
-
additional information
additional information
-
KM-value for carboxymethyl cellulase is 1.3 g/l, KM-value for beta-glucan is 1.14 g/l, endoglucanase EG44
-
additional information
additional information
-
KM-value for carboxymethyl cellulase is 11 g/l, KM-value for beta-glucan is 0.12 g/l, endoglucanase EG28
-
additional information
additional information
-
KM-value for carboxymethyl cellulase is 2.4 g/l, KM-value for beta-glucan is 1.8 g/l, endoglucanase EG51
-
additional information
additional information
-
KM-value for carboxymethyl cellulase is 4.6 g/l, KM-value for beta-glucan is 0.17 g/l, endoglucanase EG60
-
additional information
additional information
-
KM-value for carboxymethyl cellulase is 5.4 g/l, KM-value for beta-glucan is 3.0 g/l, endoglucanase EG25
-
additional information
additional information
-
KM-value for carboxymethyl cellulase is 6.8 g/l, KM-value for beta-glucan is 0.46 g/l, endoglucanase EG47
-
additional information
additional information
-
KM-value for carboxymethyl cellulose is 45 mg/ml
-
additional information
additional information
-
KM-value for carboxymethyl cellulose: 3.5% w/v (enzyme from strain NIAB442), 2.5% w/v (enzyme from deoxyglucose- and streptomycin-resistant mutant 51 Smr)
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
-
additional information
additional information
-
Km-value for carboxymethyl cellulose is 16.07 mg/ml
-
additional information
additional information
-
KM-value for carboxymethylcellulase is 6.25 mg/ml at pH 5.0, 50°C
-
additional information
additional information
-
Km-value is 0.1 mg/ml glucose for hydrolysis of carboxymethyl cellulose
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for digestion of carboxymethyl cellulose KM-value: 5.4 mg/ml and Vmax-value: 3118.4 U/mg
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Km of 84.4 mg/ml for carboxymethyl cellulose as a substrate
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Km of 84.4 mg/ml for carboxymethyl cellulose as a substrate
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KM value: 20 mg carboxymethylcellulose/ml. Vmax value: 854 U/mg protein, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose
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Km-values: 4.8 mg/ml for carboxymethyl cellulose at 50 and 60°C, 4.5 mg/ml for carboxymethyl cellulose at 70°C, 4.2 mg/ml for carboxymethyl cellulose at 80°C, and 18.4 mg/ml for amorphous cellulose
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kinetics, native enzyme
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kinetics of Rucel5B with different substrates, overview
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KM-values of N-terminal and C-terminal deletion mutants
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KM-values of N-terminal and C-terminal deletion mutants
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Km and Vmax are 0.704 mg/ml and 0.02985 mmol/ml/min, respectively
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Km for carboxymethyl cellulose is 6.4 mg/ml, pH 5.5, 45°C, recombinant enzyme, Michaelis-Menten kinetics
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KM is 14.3 mg/ml, substrate carboxymethyl cellulose, recombinant enzyme, pH 5.0, 60°C
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KM is 14.3 mg/ml, substrate carboxymethyl cellulose, recombinant enzyme, pH 5.0, 60°C
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Thermochaetoides thermophila
Michaelis-Menten kinetics, Km is 9.82 mg/ml for barley beta-1,4-D-glucan
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the enzyme has a Km of 0.1299 mg/ml and a Vmax of 0.097 mol/min/ml, Michaelis-Menten kinetic modeling
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thermodynamic kinetics of the endoglucanase at different concentrations of butanol
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Vmax and Km are 339 U/mg and 2.57 mg/ml, respectively, Michaelis-Menten kinetics and thermodynamic analysis, overview
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oat spelt xylan
Km value 2.75 mg/ml, pH 5.0, temperature not specified in the publication
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raffinose
Km value 0.011 mg/ml, pH 5.0, 60°C
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raffinose
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Km value 0.011 mg/ml, pH 5.0, 60°C
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xyloglucan
Km value 12.8 mg/ml, pH 6.5, 50°C