Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Rasamsonia emersonii |
Protein Variants | Comment | Organism |
---|---|---|
G266C/D320C | introduction of an additional disulfide bridge, decrease in activity | Rasamsonia emersonii |
G4C/A72C | introduction of an additional disulfide bridge, improves thermostability | Rasamsonia emersonii |
G4C/A72C/N54C/P191C/T243C/A375C | introduction of 3 additional disulfide bridges, improves thermostability | Rasamsonia emersonii |
N54C/P191C | introduction of an additional disulfide bridge, improves thermostability | Rasamsonia emersonii |
Q190C/I200C | introduction of an additional disulfide bridge, decrease in activity | Rasamsonia emersonii |
T243C/A375C | introduction of an additional disulfide bridge, improves thermostability | Rasamsonia emersonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
4-methylumbelliferyl beta-D-lactoside | mutant N54C/P191C, pH 5.0, 22°C | Rasamsonia emersonii | |
0.23 | - |
4-methylumbelliferyl beta-D-lactoside | mutant G4C/A72C/N54C/P191C/T243C/A375C, pH 5.0, 22°C | Rasamsonia emersonii | |
0.26 | - |
4-methylumbelliferyl beta-D-lactoside | mutant G4C/A72C, pH 5.0, 22°C | Rasamsonia emersonii | |
0.31 | - |
4-methylumbelliferyl beta-D-lactoside | deglycosylated wild-type, pH 5.0, 22°C | Rasamsonia emersonii | |
0.31 | - |
4-methylumbelliferyl beta-D-lactoside | mutant T243C/A375C, pH 5.0, 22°C | Rasamsonia emersonii | |
0.33 | - |
4-methylumbelliferyl beta-D-lactoside | wild-type, pH 5.0, 22°C | Rasamsonia emersonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | Q8TFL9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | x * 51475, MALDI-TOF of recombinant protein, x * 47206, MALDI-TOF of recombinant deglycosylated protein | Rasamsonia emersonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-lactoside + H2O | - |
Rasamsonia emersonii | 4-methylumbelliferone + D-lactose | - |
? | |
avicel + H2O | - |
Rasamsonia emersonii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46842, calculated, x * 66000, SDS-PAGE, x * 51475, MALDI-TOF of recombinant protein, x * 47206, MALDI-TOF of recombinant deglycosylated protein | Rasamsonia emersonii |
Synonyms | Comment | Organism |
---|---|---|
Cel7A | - |
Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
74.5 | - |
melting temperature, deglycosylated wild-type | Rasamsonia emersonii |
75 | - |
melting temperature, wild-type | Rasamsonia emersonii |
78.5 | - |
melting temperature, mutant N54C/P191C | Rasamsonia emersonii |
79 | - |
melting temperature, mutant T243C/A375C | Rasamsonia emersonii |
80 | - |
melting temperature, mutant G4C/A72C | Rasamsonia emersonii |
84 | - |
melting temperature, mutant G4C/A72C/N54C/P191C/T243C/A375C | Rasamsonia emersonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
4-methylumbelliferyl beta-D-lactoside | mutant G4C/A72C/N54C/P191C/T243C/A375C, pH 5.0, 22°C | Rasamsonia emersonii | |
0.47 | - |
4-methylumbelliferyl beta-D-lactoside | mutant T243C/A375C, pH 5.0, 22°C | Rasamsonia emersonii | |
0.5 | - |
4-methylumbelliferyl beta-D-lactoside | mutant G4C/A72C, pH 5.0, 22°C | Rasamsonia emersonii | |
0.52 | - |
4-methylumbelliferyl beta-D-lactoside | wild-type, pH 5.0, 22°C | Rasamsonia emersonii | |
0.55 | - |
4-methylumbelliferyl beta-D-lactoside | deglycosylated wild-type, pH 5.0, 22°C | Rasamsonia emersonii | |
0.6 | - |
4-methylumbelliferyl beta-D-lactoside | mutant N54C/P191C, pH 5.0, 22°C | Rasamsonia emersonii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rasamsonia emersonii | isoelectric focusing | - |
4 |
Rasamsonia emersonii | calculated | - |
4.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
4-methylumbelliferyl beta-D-lactoside | mutant T243C/A375C, pH 5.0, 22°C | Rasamsonia emersonii | |
1.6 | - |
4-methylumbelliferyl beta-D-lactoside | wild-type, pH 5.0, 22°C | Rasamsonia emersonii | |
1.8 | - |
4-methylumbelliferyl beta-D-lactoside | deglycosylated wild-type, pH 5.0, 22°C | Rasamsonia emersonii | |
1.9 | - |
4-methylumbelliferyl beta-D-lactoside | mutant G4C/A72C, pH 5.0, 22°C | Rasamsonia emersonii | |
1.9 | - |
4-methylumbelliferyl beta-D-lactoside | mutant G4C/A72C/N54C/P191C/T243C/A375C, pH 5.0, 22°C | Rasamsonia emersonii | |
2.8 | - |
4-methylumbelliferyl beta-D-lactoside | mutant N54C/P191C, pH 5.0, 22°C | Rasamsonia emersonii |