3.2.1.4: cellulase
This is an abbreviated version!
For detailed information about cellulase, go to the full flat file.
Word Map on EC 3.2.1.4
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3.2.1.4
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cellulose
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trichoderma
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biomass
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reesei
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xylanase
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cellulolytic
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saccharification
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lignocellulosic
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cellobiose
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straw
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lignin
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polysaccharide
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aspergillus
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glycoside
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beta-glucosidase
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corn
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biofuels
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pectinase
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xylan
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hemicellulose
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bagasse
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penicillium
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endoglucanases
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bioethanol
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xylose
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amylase
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polygalacturonase
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sugarcane
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carbohydrate-binding
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feedstock
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viride
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glucans
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thermocellum
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bran
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carboxymethylcellulose
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microcrystalline
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exoglucanase
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cellulose-binding
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laccase
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biorefinery
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rumen
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biotechnology
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analysis
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novozyme
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silage
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endoxylanase
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compost
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industry
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textile industry
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food industry
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biofuel production
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agriculture
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deconstruct
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xylobiose
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diagnostics
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harzianum
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degradation
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chrysosporium
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synthesis
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detergent
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environmental protection
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xyloglucans
- 3.2.1.4
- cellulose
- trichoderma
- biomass
- reesei
- xylanase
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cellulolytic
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saccharification
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lignocellulosic
- cellobiose
- straw
- lignin
- polysaccharide
- aspergillus
- glycoside
- beta-glucosidase
- corn
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biofuels
- pectinase
- xylan
- hemicellulose
- bagasse
- penicillium
- endoglucanases
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bioethanol
- xylose
- amylase
- polygalacturonase
- sugarcane
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carbohydrate-binding
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feedstock
- viride
- glucans
- thermocellum
- bran
- carboxymethylcellulose
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microcrystalline
- exoglucanase
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cellulose-binding
- laccase
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biorefinery
- rumen
- biotechnology
- analysis
-
novozyme
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silage
- endoxylanase
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compost
- industry
- textile industry
- food industry
- biofuel production
- agriculture
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deconstruct
- xylobiose
- diagnostics
- harzianum
- degradation
- chrysosporium
- synthesis
- detergent
- environmental protection
- xyloglucans
Reaction
Synonyms
(1->4)-beta-D-glucan 4-glucanohydrolase, 1,4-beta-D-endoglucanase, 1,4-beta-D-glucan-4-glucanohydrolase, 168cel5, 9.5 cellulase, Abscission cellulase, AEG, Ag-EGase III, AgCMCase, alkali cellulase, Alkaline cellulase, AnCel5A, AtCel5, ATEG_07420, avicelase, BC-EG70a, Bc22Cel, BCE1, BcsZ, beta-1,4-endoglucan hydrolase, beta-1,4-endoglucanase, beta-1,4-glucanase, Bgl7A, bifunctional endoglucanase/xylanase, BlCel9, BP_Cel9A, Bx-ENG-1, Bx-ENG-2, Bx-ENG-3, C4endoII, carbomethyl cellulase, Carboxymethyl cellulase, Carboxymethyl-cellulase, carboxymethylcellulase, Cat 1, Caylase, CBH45-1, cbh6A, CBHI, CBHII, CcCel6C, CEL1, Cel1 EGase, Cel12A, Cel1753, Cel28a, Cel44A, Cel45A, Cel48A, Cel5, Cel5A, cel5B, Cel5E, Cel6A, Cel6A (E2), Cel6B, Cel6C, CEL7, Cel7A, Cel7B, Cel8, Cel8A, Cel8M, Cel8Y, Cel9A, CEL9A-50, CEL9A-65, Cel9A-68, CEL9A-82, Cel9A-90, Cel9B, CEL9C1, Cel9K, Cel9M, Cel9Q, CelA, CelB, CelC2 cellulase, CelCM3, CelDR, CelE, CelF, Celf_1230, Celf_3184, CelG, CelG endoglucanase, CelI15, cell-bound bacterial cellulase, CelL15, CelL73, cellic Ctec2, cellobiohydrolase, cellobiohydrolase I, celluase A, Celluclast, celludextrinase, Cellulase, cellulase 12A, cellulase A, cellulase A 3, cellulase Cel48F, cellulase Cel9A, cellulase Cel9M, cellulase CelC2, cellulase CelE, cellulase CM3, Cellulase E1, Cellulase E2, Cellulase E4, Cellulase E5, cellulase EGX, cellulase II, cellulase III, cellulase K, Cellulase SS, cellulase T, Cellulase V1, cellulase Xf818, cellulases I, cellulases III, cellulosin AP, Cellulysin, CelP, celS, CelStrep, celVA, CelX, CenA, CenC, CfCel6A, CfCel6C, CfEG3a, CHU_1280, CHU_2103, CjCel9A, Clocel_2741, CMCase, CMCase-I, CMCax, CMcellulase, Csac_1076, Csac_1078, CSCMCase, ctCel9D-Cel44A, CTendo45, ctendo7, CtGH5, Cthe_0435, CTHT_0045780, CX-cellulase, CyPB, DCC85_10145, DK-85, Dockerin type 1, Dtur_0671, E1 endoglucanase, Econase, EfPh, EG I, EG III, EG1, EG12, EG2, EG25, EG271, EG28, EG3, EG35, EG44, EG47, EG51, Eg5a, EG60, EGA, EGase, EGase II, EGB, EGC, EGCCA, EGCCC, EGCCD, EGCCF, EGCCG, EGD, EGE, EGF, egGH45, EGH, EGI, EGII, EGII/Cel5A, EGIV, EGL, EGL 1, Egl-257, Egl1, Egl499, Egl5a, EglA, eglB, EglC, EGLII, EglS, EGM, EGPf, EGPh, EGSS, EgV, EGX, EGY, EGZ, endo-1,4-B-glucanase, endo-1,4-beta-D-glucanase, endo-1,4-beta-glucanase, endo-1,4-beta-glucanase 1, endo-1,4-beta-glucanase 2, endo-1,4-beta-glucanase E1, endo-1,4-beta-glucanase V1, endo-beta-1,3-1,4-glucanase, endo-beta-1,4-glucanase, endo-beta-1,4-glucanase 1, endo-beta-1,4-glucanase 2, endo-beta-1,4-glucanase CMCax, endo-beta-1,4-glucanase EG27, endo-beta-1,4-glucanase EG45, endo-beta-D-1,4-glucanohydrolase, endo-beta-glucanase, endo-glucanase, ENDO1, ENDO2, endocellulase, endocellulase E1, endocellulases I, endocellulases II, endocellulases III, endocellulases IV, endogenous beta-1,4-endoglucanase, endogenous cellulase, endoglucanase, endoglucanase 1, endoglucanase 35, endoglucanase 47, endoglucanase CBP105, endoglucanase Cel 12A, endoglucanase Cel 5A, endoglucanase Cel 7B, endoglucanase Cel5A, endoglucanase Cel6A, endoglucanase D, endoglucanase EG-I, endoglucanase EG25, endoglucanase EG28, endoglucanase EG44, endoglucanase EG47, endoglucanase EG51, endoglucanase EG60, endoglucanase H, endoglucanase II, endoglucanase IIa, endoglucanase IV, endoglucanase L, endoglucanase M, endoglucanase V, endoglucanase Y, endolytic cellulase, EngA, EngH, EngL, EngM, engXCA, EngY, EngZ, family 7 cellobiohydrolase, FI-CMCASE, FnCel5A, FpCel45, Fpcel45a, GE40, GE40 endoglucanase, GH12 endo-1,4-beta-glucanase, GH124 endoglucanase, GH45 endoglucanase, gh45-1, GH5 cellulase, GH5 endoglucanase, GH6 endoglucanase, GH7 endoglucanase, GH9 termite cellulase, Glu1, Glu2, glycoside hydrolase family 9 endoglucanase, GtGH45, Lp-egl-1, manganese dependent endoglucanase, Maxazyme, Meicelase, mesophilic endoglucanase, mgCel6A, More, MtGH45, nmGH45, Onozuka R10, pancellase SS, PaPopCel1, PF0854, PH1171, PttCel9A, RCE1, RCE2, Roth 3056, RtGH124, Rucel5B, Sl-cel7, Sl-cel9C1, SnEG54, SoCel5, ssgluc, SSO1354, SSO1354 enzyme, SSO1354 protein, SSO1949, SSO2534, STCE1, Sumizyme, T12-GE40, TC Serva, TeEG-I, TeEgl5A, TfCel9A, ThEG, theme C glycoside hydrolase family 9 endo-beta-glucanase, Thermoactive cellulase, thermostable carboxymethyl cellulase, THITE_2110957, TM_1525, TM_1751, umcel5G, umcel9y-1, Vul_Cel5A
ECTree
3.2.1.4 cellulaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 3.2.1.4 - cellulase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
10 - 70
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purified native enzyme, highly stable in the temperature range of 10-40°C, the activity declines rapidly as the temperature increases above 60°C, 12% activity remains at 70°C after 4 h. At 10°C, the enzyme exhibits no loss of activity, even after 24 h
100
101
melting-temperature of the mutant enzymes P74C/C106S, P74C and C106S
110
maximal activity at 95°C and retains 49% activity at 110°C
115
purified recombinant His-tagged enzyme, pH 6.0, half-life is 9.1 min
25 - 55
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purified recombinant enzyme, very stable at 25°C, loss of about 13.2% activity at 35°C after 2 h. The enzyme retains 10.4% activity at 45°C after 2 h, and is completely inactivated at 55°C after 30 min
36
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30 min, 50% loss of activity of cellulase 4.8, 25% loss of activity of cellulase 4.5
37
39 - 60
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the purified enzyme is stable at 39-60°C and pH 4.0-6.0 for 60 min
4 - 45
the enzyme has an estimated low melting temperature of 45°C, about 50% activity remaining at 4°C, it is identified to be cold-adapted
40
45
50
50 - 60
50 - 70
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purified native enzyme, pH 5.0, the enzyme retains more than 50% activity after incubation at 65°C and 70°C for 180 min, no major loss in activity at 50°C
54
55
57
60
60 - 70
65
70
75
79
80
83
85
90
90 - 100
95
95 - 96
Tm-value for mutant enzymes C106A/C159A/C372A/C412A, C106A/C159A and C372A/C412A
97
additional information
100
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10 min, cellulase II-A retains 27% of the original activity, cellulase II-B retains 41% of the original activity
100
purified recombinant His-tagged enzyme, pH 6.0, half-life is 46 min
40
purified recombinant enzyme, completely stable to slightly activated after 48 h
45
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2 h, cellulase II-B is stable over the pH-range 4.5-6.0, cellulase II-A is unstable, except at pH 5.5-6.0
50
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half life for carboxymethylcellulase, filter paper cellulase, and beta-glucosidase is approximately 240 h
50
purified recombinant enzyme, 80% activity remaining after 48 h
50
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CMCase, 1 day, no loss of activity, 46% activity remaning after 26 days, inactivation after 40 days
50
t1/2: 173 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity
50
t1/2: 693 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity
50
15 h, wild-type enzyme and mutant enzymes Y206F, D58A and Y318A are stable, mutant enzyme D58N loses about 20% of its inital activity
50
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48 h, 88% and 98% residual activity on filter paper and carboxymethyl cellulose, respectively
50
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half-life of free enzyme is 3.0 h, half-life of the enzyme immobilized in calcium-alginate beads is 4.6 h
50 - 60
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CMCase, 100% activity after 1 day, 80% activity remaining after 40 days
55
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10 min, about 30% loss of activity with barley beta-glucan and about 60% loss of activity with carboxymethylcellulose
60
10 min, mutant lacking the IgG-like domain stable up to 60°C
60
purified recombinant enzyme, 50% activity remaining after 3 h, 20% after 6 h, and inactivation after 12 h
60
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CMCase, 97% activity remaining after 1 day, below 5% activity remaining after 40 days
60
30 min, complete loss of activity in absence of NaCl, no loss in presence of 2.5 M NaCl
60
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10 min, about 50% loss of activity with barley beta-glucan and about 75% loss of activity with carboxymethylcellulose
60
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30 min, more than 50% loss of activity of the catalytic domain and of the whole enzyme
60
t1/2: 16 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity
60
t1/2: 347 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity
60
15 h, wild-type enzyme is stable, mutant enzyme D58A loses about 20% of initial activity, mutant enzymes Y206F, D58N, D58N and Y318A lose about 10% of the inital activity
60
Thermochaetoides thermophila
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t1/2: 18 min (without substrate), carboxymethyl cellulose partially protects the enzyme from heat inactivation
60
Thermochaetoides thermophila
120 min, enzyme retains about 85% of its initial activity, xylanase ativity
60
Thermochaetoides thermophila
120 min, enzyme retains more than 80% of its initial activity, endoglucanase activity
60
Thermochaetoides thermophila
purified recombinant His-tagged enzyme, 90% activity remaining after 120 min
60
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half-life of free enzyme is 1.2 h, half-life of the enzyme immobilized in calcium-alginate beads is 5.4 h
60 - 70
purified recombinant His-tagged enzyme, half-life of enzyme is 168 h at 60°C and 3 h at 70°C, respectively
65
15 h, wild-type enzyme loses about 10% of initial activity, mutant enzyme D58N and Y206F loses about 20% of maximal activity, mutant enzyme D58A loses about 90% of initial activity, mutant enzyme Y318A loses about 30% of initial activity
70
72 h, presence of 0.1% Tween 20, no loss of activity
70
purified enzyme, 48% activity remaining after 5 min, 30% after 60 min
70
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CMCase, 89% activity after 1 day, complete loss of activity after 20 days
70
2 h, the wild-type enzyme retains 38% activity, the enzyme mutants retain 52% and 58% activity, respectively
70
t1/2: 15 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity
70
t1/2: 231 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity
70
wild-type enzyme loses about 50% of initial activity, mutant enzyme D58A loses about 20% of initial activity, mutant enymes D58A, Y206F and Y318A nearly completely lose activity
70
Thermochaetoides thermophila
60 min, enzyme retains 66.2% of its initial activity, xylanase ativity
70
Thermochaetoides thermophila
60 min, enzyme retains 74.3% of its initial activity, endoglucanase activity
70
Thermochaetoides thermophila
purified recombinant His-tagged enzyme, 74.3% remaining after 60 min, half-life of endoxylanase activity is 110 min, 30% activity remaining after 120 min
75
-
about 20% loss of activity after 30 min, about 50% loss of activity after 2 h, 51000 Da subunit from the multicomponent cellulase complexes
75
72 h, presence of 0.1% Tween 20, 75% of initial activity
75
-
denaturation, presence of tris-(2-hydroxyethyl)-methylammonium methylsulfate
75
CelDR retains 70% of its maximum activity at 75°C after incubation for 30 min
75
-
half-life: endocellulases I (104 min), endocellulases II (93 min), endocellulases III (75 min), endocellulases IV (66 min)
75
purified recombinant enzyme, completely stable for 1 h, and 98% activity remaining after 2 h
75
purified recombinant His-tagged enzyme, pH 6.0, completely stable for 48 h
80
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M44-11 mutant shows higher stability than others and retains more than 50% of its activity after incubation at 80°C for 1 h
80
purified recombinant His-tagged enzyme, at least 91.4% activity remains after 1 h
80
purified recombinant enzyme, completely stable for 1 h, and 90% activity remaining after 2 h
80
half-life: 8 h at pH 1.8, 160 min at pH 1. At neutral pH, the thermal inactivation rate is nearly two orders of magnitude higher than at pH 1.8
80
2 h, the wild-type enzyme retains no activity, the enzyme mutants retain 15% and 22% activity, respectively
80
t1/2: 12 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity
80
t1/2: 63 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity
80
Thermochaetoides thermophila
60 min, enzyme retains 61.3% of its initial activity, endoglucanase activity
80
Thermochaetoides thermophila
60 min, enzyme retains about 50% of its initial activity, xylanase ativity
80
Thermochaetoides thermophila
purified recombinant His-tagged enzyme, 61.3% activity remaining after 60 min, half-life of endoxylanase activity is 80 min, 18% activity remaining after 120 min
80
presence of 96%glycerol, plus 0.1% H2SO4, half-life 4.5 min
85
purified recombinant enzyme, completely stable for 1 h, and 84% activity remaining after 2 h
90
-
enzyme is able to sustain 50% of its activity when heated at 90°C for 5 h
90
enzyme retains approximately 50% of its maximum activity after incubating at 90°C for 1 h
90
t1/2: 12 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity
90
t1/2: 20 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity
90
Thermochaetoides thermophila
40 min, enzyme retains about 28% of its initial activity, endoglucanase activity
90
Thermochaetoides thermophila
60 min, enzyme retains about 30% of its initial activity, xylanase ativity
90
Thermochaetoides thermophila
purified recombinant His-tagged enzyme, half-life of endoxylanase activity is 30 min, 20% activity remaining after 60 min, after 120 min
90
-
5 min, aqueous buffer, 87% residual activity, acidified (0.1% (w/w) H2SO4) glycerol, 35% residual activity, acidified ethylene glycol, 36% residual activity
90
5 min, aqueous buffer, 87% residual activity, acidified (0.1% (w/w) H2SO4) glycerol or glycerol, 80% residual activity, acidified ethylene glycol, 36% residual activity
90
recombinant enzyme NMgh45, 68% activity remains after 1 h, 40% after 2 h
additional information
enzyme melting transition occurs at 72°C in presence of EDTA, Ca2+ does not affect the melting temperature
additional information
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enzyme melting transition occurs at 72°C in presence of EDTA, Ca2+ does not affect the melting temperature
additional information
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more stable to heat treatment at pH 8.0 than at pH 4.0
additional information
high thermostability (Topt: 79.8°C, half-life 48 h)
additional information
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high thermostability (Topt: 79.8°C, half-life 48 h)
additional information
-
slight activation of cellulase B after 10 min at 40°C
additional information
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free enzyme shows an activity of 75% of the initial activity after 30 min, and even an activity of 40% remained after 6 h, both measured at 70°C
additional information
the N-terminalbeta-sheet is critical for enzyme thermostability
additional information
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the N-terminalbeta-sheet is critical for enzyme thermostability
additional information
mechanism of improved thermostability of hybrid mutant enzymes compared to parent wild-type enzymes
additional information
-
mechanism of improved thermostability of hybrid mutant enzymes compared to parent wild-type enzymes
additional information
mechanism of improved thermostability of hybrid mutant enzymes compared to parent wild-type enzymes
additional information
-
mechanism of improved thermostability of hybrid mutant enzymes compared to parent wild-type enzymes
additional information
-
low pH is the major factor that limits endoglucanase stability in glycerol or ethylene glycol at elevated temperatures
additional information
low pH is the major factor that limits endoglucanase stability in glycerol or ethylene glycol at elevated temperatures
