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1-butyl-3-methylimidazolium chloride
92.8% and 43.8% activity remaining in 10% and 20% 1-butyl-3-methylimidazolium chloride
1-butyl-3-methylimidazolium methylsulfate
-
the enzyme precipitates immediately on addition to the reaction mixture
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
100 mM, 98% inhibition
4-chloromercuribenzoate
-
5 mM, 90% of initial activity
4-hydroxybenzoic acid
-
-
acetonitril
-
11.4% inhibition at 5%
acetonitrile
-
10% v/v, 89% loss of activity
Al3+
Thermochaetoides thermophila
slight inhibition at 10 mM
alpha-chymotrypsin
-
denaturant
-
benzol
-
96.2% inhibition at 5%
butanol
-
10.9% inhibition at 5%
CaCl2
-
5 mM, 52% residual activity
carboxymethylcellulose
-
binding of the cellulose-binding domain to avicel is inhibited by carboxymethylcellulose, but not by barley beta-glucan and glucose at concentration of 0.1% and 0.5%
-
cetyltrimethylammonium bromide
1 mM, 31% residual activity
CoCl2
-
5 mM, 35% loss of activity
Cr2+
-
1 mM, 27% residual activity
CuSO4
-
5 mM, 38% residual activity
D-gluconic acid lactone
-
strong inhibition of membrane-bound cellulase
Dextran
-
partial inhibition of membrane-bound cellulase
diethyl dicarbonate
-
10 mM, 17.6% residual activity
Dimethylsulfoxide
-
30% v/v, 50% inhibition
EGTA
10 mM, 24% inhibition
FeCl2
-
5 mM, 38% residual activity
glucosamine
-
inhibition of CM-cellulase activity of enzyme E1, E3a, E3b and E4
guanidinium hydrochloride
72.2% at 3%
KMnO4
-
0.1 mM, complete inhibition of cellulase II-A
lactose
-
51000 Da subunit from the multicomponent cellulase complexes
lignin
-
at 45°C, lignin derived from acid hydrolyzed liquid hot water pretreated bagasse completely adsorbes cellulolytic enzymes from Trichoderma reesei within 90 min, while lignin derived from enzyme hydrolyzed liquid hot water pretreated bagasse adsorbes only 60% of Trichoderma reesei endoglucanase, exoglucanase and beta-glucosidase activities, overview. At 30°C, adsorption of all of the enzymes is minimal and enzyme hydrolysis at 30°C approaches that at 45°C after 168 h. Simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP), both carried out at 30-32°C, offer viable options for mitigating lignin-derived inhibition effects. Method overview. Specific surface area and surface characteristics of lignocellulose particles
-
linear alkyl benzene sulfonate
LAS
methylcellulose
-
poor inhibitor of carboxymethylcellulase activity of enzyme form E3a, E3b and E4
-
MgCl2
-
5 mM, 52% residual activity
MnCl2
-
5 mM, 67% loss of activity
Monoiodoacetic acid
1 mM, 10% inhibition
NaN3
0.1%, 45.2% inhibition of activity with carboxymethyl cellulose, 12.25% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
o-phenanthroline
1 mM, 75% inhibition
p-chloromercuribenzoate
0.01 mM, 20% inhibition
Pb(CH3COO)2
-
34% inhibition at 1 mM
PbCl2
-
5 mM, 37% loss of activity
Phenylmethyl sulfonylfluoride
-
1 mM, 30% residual activity
phenylmethylsulfonyl fluoride
sodium dodecyl benzene sulfonate
-
-
sodium picryl sulfate
-
2 mM, cellulase I
SrCl2
-
5 mM, 36% loss of activity
starch
-
inhibits hydrolysis of glucan
Teepol
-
detergent, 0.1%, 80% inhibition
-
Tris
Tris almost completely suppresses CtCel9Q hydrolase activity, a Tris molecule is bound to three catalytic residues of CtCel9Q and occupies subsite -1 of the CtCel9Q active-site cleft, enzyme mutant crystal structure analysis, overview
Tween 20
31% inhibition at 20%
Tween-20
-
1%, 79% residual activity
2-mercaptoethanol
-
10 mM, 51% residual activity
2-mercaptoethanol
-
70% of initial activity
2-mercaptoethanol
-
5 mM, 82.3% residual activity, 50%, 48 h, crude enzyme preparation
Ag+
5 mM, no residual activity
Ag+
-
1 mM, 75% inhibition
Ag+
1 mM, 86% residual activity
Ag+
-
0.1 mM, strong inhibition
Ag+
-
AgNO3, endoglucanase E1 and E2
Ag+
Thermochaetoides thermophila
-
-
Ag+
Thermochaetoides thermophila
-
1 mM, 1% residual activity
Ag+
Thermochaetoides thermophila
23% inhibition at 1 mM, 68% inhibition at 5 mM; 5 mM, 62.5% loss of activity, substrate: xylan; 5 mM, 68.25% loss of activity, substrate: carboxymethyl cellulose
Ag+
Thermochaetoides thermophila
slight inhibition at 10 mM
Ag+
-
1 mM, partial inhibition
Ag+
complete inhibition at 5 mM
Ba2+
-
-
Ba2+
Thermochaetoides thermophila
-
-
Ba2+
Thermochaetoides thermophila
-
1 mM, 38% residual activity
Ba2+
-
1 mM, 79%inhibition
Ca2+
-
-
Ca2+
10 mM, 90% residual activity
Ca2+
5 mM, 52% residual activity
Ca2+
-
1 mM, slight inhibition
Ca2+
-
slight inhibition of endoglucanase E2 activity
Ca2+
-
5 mM, 76% residual activity
Ca2+
23.8% inhibition at 5 mM
Ca2+
-
1 mM, 74%inhibition
Ca2+
12.7% inhibition at 10 mM
Cd2+
10 mM, 16.5% residual activity
Cd2+
-
1 mM CdCl2, complete inhibition
Cd2+
2 mM, 70% of initial activity
Cd2+
10 mM, almost 30% loss of activity
Cd2+
-
14% inhibition at 10 mM
cellobiose
-
-
cellobiose
-
51000 Da subunit from the multicomponent cellulase complexes
cellobiose
Bacillus cellulyticus K-12
-
inhibits hydrolysis of carboxymethylcellulose and avicel
cellobiose
-
80 mM, 14% loss of activity, endoglucanase Z
cellobiose
very low activity against 4-methyl-umberriferyl-beta-D-lactoside is almost totally inhibited by 0.005 M cellobiose
cellobiose
-
5 mM, complete inhibition of carboxymethyl cellulose hydrolysis
cellobiose
-
inhibits hydrolysis of carboxymethylcellulose and avicel
cellobiose
the non-complete saccharification of cellulose by thr enzyme seems to be due to product-feedback inhibition by cellobiose
cellobiose
-
inhibition of endoglucanase E3a, E3b and E4
cellobiose
-
endoglucanase Cel 5A; endoglucanase Cel 7B
Co2+
5 mM, 90% of initial activity
Co2+
-
1 mM CoCl2, 44% inhibition
Co2+
5 mM, 37% residual activity
Co2+
-
5 mM, 76% residual activity
Co2+
MG570051
2 mM, less than 50% of initial activity
Co2+
-
5 mM, 43% of initial activity
Co2+
10 mM, almost 30% loss of activity
Co2+
Thermochaetoides thermophila
5 mM, 2% loss of activity, substrate: xylan
Co2+
Thermochaetoides thermophila
activates 38% at 2 mM, inhibits 20% at 10 mM
Co2+
-
1 mM, 62% residual activity
Co2+
-
1 mM, represses the enzyme activity up to 37%
Co2+
10.1% inhibition at 10 mM
Cr3+
-
Cr3+
5 mM, 34% residual activity
Cr3+
Thermochaetoides thermophila
-
Cu2+
-
inhibition of cellulose hydrolysis, no carboxymethylcellulose hydrolysis
Cu2+
10 mM, 27% residual activity
Cu2+
Bacillus cellulyticus K-12
-
CuCl2
Cu2+
-
1 mM, 40% residual activity
Cu2+
-
1 mM CuCl2, 41% inhibition
Cu2+
-
1 mM, 18% residual activity
Cu2+
-
5 mM, about 30% inhibition
Cu2+
-
1 mM, 72% of initial activity
Cu2+
5 mM, 38% residual activity
Cu2+
10 mM, almost 30% loss of activity
Cu2+
-
1 mM CuCl2, 55% inhibition
Cu2+
-
6.5% inhibition at 1 mM
Cu2+
5 mM, 41% residual activity
Cu2+
-
22% inhibition at 10 mM
Cu2+
5 mM, 1.6fold activation of activity with carboxymethyl cellulose, 16.4% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
Cu2+
Thermochaetoides thermophila
-
-
Cu2+
Thermochaetoides thermophila
-
1 mM, 10% residual activity
Cu2+
Thermochaetoides thermophila
5 mM, complete loss of activity, substrate: carboxymethyl cellulose; 5 mM, complete loss of activity, substrate: xylan; 75% inhibition at 1 mM, complete inhibition at 5 mM
Cu2+
Thermochaetoides thermophila
complete inhibition at 2-10 mM
Cu2+
-
5 mM, 12% residual activity
Cu2+
-
1 mM, partial inhibition
Cu2+
complete inhibition at 5 mM
Cu2+
-
1 mM, 12% loss of activity
Cu2+
37.7% inhibition at 10 mM
dithiothreitol
-
10 mM, 67% residual activity
dithiothreitol
-
78% inhibition
EDTA
5 mM, 76% of initial activity
EDTA
10 mM, 58% residual activity
EDTA
-
5 mM, 75% loss of activity
EDTA
-
10 mM, 28% residual activity
EDTA
-
27% residual activity
EDTA
5 mM, complete inhibition of mutant enzyme DELTA1-90
EDTA
-
5 mM, 40% inhibition
EDTA
-
1 mM, 17% residual activity
EDTA
-
5 mM, 17.9% residual activity, 50%, 48 h, crude enzyme preparation
EDTA
-
10 mM, 11.2% residual activity
EDTA
1 mM, 91% residual activity
EDTA
5 mM, 68% residual activity
EDTA
-
5 mM, 63% of initial activity
EDTA
-
1 mM, 55% inhibition
EDTA
5 mM, 46% residual activity
EDTA
5 mM, 90.6% inhibition of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
EDTA
-
1 mM, 66% residual activity
EDTA
5 or 10 mM of EDTA reduce barley beta-glucan hydrolysis by 39% and 34%, respectively
EDTA
-
50 mM, 14% inhibition
ethanol
5%, 16% inhibition of activity with carboxymethyl cellulose, 24% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
ethanol
-
30% v/v, 92% loss of activity
Fe2+
-
-
Fe2+
-
1 mM, 55% inhibition
Fe2+
10 mM, 30% residual activity
Fe2+
-
10% inhibition at 5 mM
Fe2+
-
1 mM, 37% residual activity
Fe2+
-
1 mM, 65% residual activity
Fe2+
-
complete inhibition
Fe2+
-
5 mM, 77% of initial activity
Fe2+
10 mM, complete inhibition
Fe2+
-
1 mM FeCl2, 13% inhibition
Fe2+
-
2 mM, 67% inhibition
Fe2+
5 mM, 2.9fold activation of activity with carboxymethyl cellulose, 6.7% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
Fe2+
Thermochaetoides thermophila
-
-
Fe2+
Thermochaetoides thermophila
-
1 mM, no residual activity
Fe2+
-
complete inhibition
Fe2+
complete inhibition at 5 mM
Fe3+
-
-
Fe3+
-
39% inhibition by 0.1 mM, 52% inhibition by 1 mM
Fe3+
-
5 mM, 5% residual activity
Fe3+
-
1.0 mM, significant inhibition
Fe3+
5 mM, 1.1fold activation of activity with carboxymethyl cellulose, 9.1% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
Fe3+
Thermochaetoides thermophila
14% inhibition at 1 mM, 70% inhibition at 5 mM; 5 mM, 70.3% loss of activity, substrate: carboxymethyl cellulose; 5 mM, 78.5% loss of activity, substrate: xylan
Fe3+
Thermochaetoides thermophila
complete inhibition at 2-10 mM
Fe3+
-
1 mM, 62% residual activity
Fe3+
61.4% inhibition at 5 mM
Fe3+
-
1 mM, 73% inhibition
glucose
-
162 mM, 23% loss of activity, endoglucanase Z
glucose
-
moderate inhibition
glucose
-
end-product inhibition, 70% loss of activity by 0.3% glucose
glucose
-
29 mM, 12% inhibition of endoglucanase E4, no inhibition of endoglucanase E1 and E3a
Hg2+
-
inhibition of cellulose hydrolysis and carboxymethylcellulose hydrolysis
Hg2+
-
1 mM, 67% inhibition
Hg2+
-
1 mM, 67% inhibition
Hg2+
-
73.5% inhibition at 5 mM
Hg2+
Bacillus cellulyticus K-12
-
HgCl2
Hg2+
-
1 mM, 30% residual activity
Hg2+
-
1 mM HgCl2, complete inhibition
Hg2+
-
21% inhibition at 1 mM
Hg2+
5 mM, 45% residual activity
Hg2+
-
5 mM, 10% residual activity
Hg2+
-
1 mM, 40% residual activity
Hg2+
-
5 mM, 46% residual activity
Hg2+
-
5 mM, 95% inhibition
Hg2+
-
1 mM, 12% residual activity
Hg2+
-
5 mM, 21.9% residual activity, 50%, 48 h, crude enzyme preparation
Hg2+
-
5 mM, 58% inhibition
Hg2+
-
1.0 mM, significant inhibition
Hg2+
1 mM, complete inhibition
Hg2+
-
1 mM, no residual activity
Hg2+
-
1 mM, complete loss of activity
Hg2+
-
Hg(acetate)2, restored by Cys or Cl-
Hg2+
-
1 mM, strong inhibition
Hg2+
10 mM, complete inhibition
Hg2+
-
1 mM HgCl2, complete inhibition
Hg2+
-
2 mM, 20% inhibition
Hg2+
-
endoglucanase E1 and E2; HgCl2
Hg2+
Thermochaetoides thermophila
-
-
Hg2+
Thermochaetoides thermophila
-
1 mM, 2% residual activity
Hg2+
-
5 mM, no residual activity
Hg2+
-
1 mM, represses the enzyme activity up to 22%
Hg2+
-
1 mM, complete inhibition
Hg2+
-
1 mM, complete inactivation
HgCl2
-
5 mM, 76% loss of activity
HgCl2
-
5 mM, 25% residual activity
iodoacetamide
-
-
Isopropanol
5%, 21.6% inhibition of activity with carboxymethyl cellulose, 53% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
K+
slight inhibition
K+
5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 13% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
K+
10.2% inhibition at 5 mM
KCl
-
5 mM, 23% loss of activity
KCl
-
5 mM, 74% residual activity
Li+
slight inhibition
Li+
2 mM, 70% of initial activity
Li+
5 mM, 71% residual activity
mercaptoethanol
-
-
mercaptoethanol
-
1 mM, slight inhibition
methanol
5%, 26.5% inhibition of activity with carboxymethyl cellulose, 13.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
methanol
-
30% v/v, 62% loss of activity
Mg2+
10 mM, 86% residual activity
Mg2+
-
31% inhibition at 1 mM
Mg2+
5 mM, 44% residual activity
Mg2+
-
2 mM, slight inhibition
Mg2+
5 mM, 62% residual activity
Mg2+
5 mM, 2.3fold activation of activity with carboxymethyl cellulose, 6.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
Mg2+
-
slight inhibition of endoglucanase E2 activity
Mg2+
Thermochaetoides thermophila
-
-
Mg2+
Thermochaetoides thermophila
-
10 mM, 12% residual activity
Mg2+
Thermochaetoides thermophila
5 mM, 51% loss of activity, substrate: xylan; 5 mM, 64.5% loss of activity, substrate: carboxymethyl cellulose; 6% inhibition at 1 mM, 64.5% inhibition at 5 mM
Mg2+
-
1 mM, 54% residual activity
Mg2+
8.1% inhibition at 5 mM
Mn2+
5 mM, 44% of initial activity
Mn2+
10 mM, 57% residual activity
Mn2+
-
54% inhibition at 0.1 mM, 71% inhibition at 1 mM
Mn2+
-
5 mM, 77% residual activity
Mn2+
-
5 mM, about 30% inhibition
Mn2+
-
5 mM, 82.5% residual activity, 50%, 48 h, crude enzyme preparation
Mn2+
1 mM, 59% residual activity
Mn2+
5 mM, 28% residual activity
Mn2+
MG570051
2 mM, less than 50% of initial activity
Mn2+
-
5 mM, 60% of initial activity
Mn2+
-
1 mM MnCl2, 18% inhibition
Mn2+
Thermochaetoides thermophila
-
-
Mn2+
Thermochaetoides thermophila
-
1 mM, 46% residual activity
Mn2+
-
1 mM, represses the enzyme activity up to 40%
Mn2+
59.3% inhibition at 5 mM
Mn2+
-
5 mM, 20% inhibition
N-bromosuccinimide
-
-
N-bromosuccinimide
-
5 mM, 56% loss of activity
N-bromosuccinimide
-
0.001 mM, complete inactivation
N-bromosuccinimide
-
1 mM, 35% residual activity
N-bromosuccinimide
1 mM, 99% inhibition
N-bromosuccinimide
-
1 mM, 90% inhibition
Na+
5 mM, 65% of initial activity
Na+
-
23% inhibition at 5 mM
Na+
5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 6% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
NaCl
5 mM, 84% residual activity
NaCl
7.1% inhibition at 5 mM
NEM
-
70% inhibition of cellulase 4.5 at 10 mM, no effect on cellulase 4.8
NH4+
-
5 mM, 10% inhibition
NH4+
-
1 mM, 84% of initial activity
NH4+
Thermochaetoides thermophila
-
-
NH4+
Thermochaetoides thermophila
-
10 mM, 9% residual activity
Ni2+
slight inhibition
Ni2+
-
1 mM NiCl2, 68% inhibition
Ni2+
5 mM, 52% residual activity
Ni2+
-
1 mM, 27% residual activity
Ni2+
Thermochaetoides thermophila
activates 8% at 2 mM, inhibits 8% at 10 mM
Ni2+
-
5 mM, no residual activity
Ni2+
12.1% inhibition at 10 mM
NiCl2
-
5 mM, 40% loss of activity
NiCl2
-
5 mM, 48% residual activity
Pb2+
5 mM, 56% of initial activity
Pb2+
-
1 mM Pb(CH3COO)-, 36% inhibition
Pb2+
-
2 mM, slight inhibition
Pb2+
no remaining activity; no remaining activity
Pb2+
-
14.9% inhibition at 1 mM
Pb2+
-
65% inhibition at 10 mM
Pb2+
Thermochaetoides thermophila
-
Pb2+
-
5 mM, 9% residual activity
Pb2+
-
1 mM, 30% residual activity
PCMB
-
-
PCMB
Bacillus cellulyticus K-12
-
-
PCMB
-
0.4 M, 60% inhibition
PCMB
-
0.1 mM, 90% loss of activity of cellulase 4.5, 50% loss of activity of cellulase 4.8
phenylmethylsulfonyl fluoride
-
10 mM, 18.1% residual activity
phenylmethylsulfonyl fluoride
-
1 mM, 60% residual activity
PMSF
-
5 mM, 10.1% residual activity, 50%, 48 h, crude enzyme preparation
PMSF
1 mM, 89% inhibition
SDS
-
-
SDS
-
42% inhibition at 5%
SDS
Bacillus cellulyticus K-12
-
10 mM, 98% inhibition
SDS
0.4%, 10% of initial activity
SDS
-
0.25 mM, complete inactivation
SDS
-
10 mM, 49.2% residual activity
SDS
0.5%, 60% residual activity
SDS
-
1%, 12% of initial activity
SDS
10 mM, 35% remaining activity; 10 mM, 54% remaining activity
SDS
-
0.1%, 56% residual activity
SDS
complete inhibition at 0.1%
SDS
-
1% v/v, almost complete loss of activity
SDS
52.1% inhibition at 1%
Sn2+
-
2 mM
sodium dodecylsulfate
5 mM, 5% of initial activity
sodium dodecylsulfate
-
1%, 73% residual activity
sodium dodecylsulfate
-
1%, 32% residual activity
sodium dodecylsulfate
-
7% residual activity
sodium dodecylsulfate
1 mM, 76% residual activity
sodium hypochlorite
-
-
Triton X-100
-
19.7% inhibition at 5%
Triton X-100
10%, 20% of initial activity
Triton X-100
0.5%, 73% residual activity
Triton X-100
slight inhibition
Urea
-
denaturant
Urea
-
10 mM, 45% residual activity
Urea
9.7% inhibition at 5 mM
Urea
82.3% inhibition at 10%, 42.3% at 7%, and 24.3 at 5%
Zn2+
-
inhibition of cellulose hydrolysis, no inhibition of carboxymethylcellulose hydrolysis
Zn2+
10 mM, 95% residual activity
Zn2+
-
1 mM ZnSO4, 88% inhibition
Zn2+
5 mM, 19% residual activity
Zn2+
-
1 mM, 21% residual activity
Zn2+
2 mM, 65% of initial activity
Zn2+
5 mM, 51% residual activity
Zn2+
-
5 mM, 22% of initial activity
Zn2+
10 mM, almost 30% loss of activity
Zn2+
-
74% inhibition at 10 mM
Zn2+
Thermochaetoides thermophila
-
-
Zn2+
Thermochaetoides thermophila
-
1 mM, 19% residual activity
Zn2+
Thermochaetoides thermophila
5 mM, 4.4% loss of activity, substrate: carboxymethyl cellulose; 5 mM, 55% loss of activity, substrate: xylan
Zn2+
-
5 mM, no residual activity
Zn2+
-
1 mM, 64% residual activity
Zn2+
complete inhibition at 5 mM
Zn2+
about 60-80% inhibition at 5-10 mM
Zn2+
73% inhibition at 10 mM
Zn2+
-
1 mM, 78%inhibition
additional information
not inhibitory: EDTA, Ca2+
-
additional information
-
more than 50% of the original activity is retained after incubation with sodium alkyl sulfonate, sodium alkyl sulfate, polyoxyethylene alkyl sulfonate, alpha-olefin sulfonate
-
additional information
-
below 10% inhibition by propan-2-ol, ethanol, methanol, tert-amyl alcohol, acetone, chloroform, hexane, toluene, ethyl acetate, glycerol, Tween 80, and Tween 20 at 5%, and by Ca2+, Mg2+, Zn2+, (NH4)2SO4, urea, NaN2, and EDTA at 5 mM
-
additional information
-
no inhibition by N-bromosuccinimide and iodoacetamide
-
additional information
-
is minutely inhibited by anionic detergent and oxidizing agent comparable with inhibition by commercial enzyme
-
additional information
BglA7 is highly resistant to both pepsin and trypsin
-
additional information
-
enzyme retains 84.1 and 71.4% of the control activity in the presence of 0.5 and 2.0 mol/l NaCl, respectively
-
additional information
-
no inhibition by EDTA, EGTA, 1,10-phenanthroline, monoiodoacetate, NEM, DTNB, PCMB and diethylpyrocarbonate
-
additional information
-
glucose (2 mM), cellobiose (1.25 mM), methylcellulose (0.5%, w/v) and gluconolactone (100 mM) do not inhibit the hydrolysis of carboxymethylcellulose by any of the endocellulases
-
additional information
-
no effect by DTT and EDTA at 10 mM
-
additional information
-
the enzyme displays optimal activity even in the existence of divalent and trivalent metallic cations: Ca2+, Mg2+, Pb2+, Cd2+, Cu2+, Ni2+ and Fe3+
-
additional information
-
no inhibition by cellobiose
-
additional information
-
gallic acid, cinnamic acid, ferulic acid, 4-coumaric acid, sinapic acid, vanilin, syringaldehyde, and 4-hydroxybenzoic acid are poor inhibitors
-
additional information
-
2,3-di-O-methylated regions in 2,3-di-O-methylated cellulose, having a trace amount of unsubstituted glucose units competitively inhibit the hydrolysis of glucose in other molecular chains
-
additional information
no or poor effect by 5 mM of SDS, DTT, and EDTA
-
additional information
organic solvents at 10-20%
-