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3.2.1.4: cellulase

This is an abbreviated version!
For detailed information about cellulase, go to the full flat file.

Word Map on EC 3.2.1.4

Reaction

cellohexaose
+
H2O
= 2 cellotriose

Synonyms

(1->4)-beta-D-glucan 4-glucanohydrolase, 1,4-beta-D-endoglucanase, 1,4-beta-D-glucan-4-glucanohydrolase, 168cel5, 9.5 cellulase, Abscission cellulase, AEG, Ag-EGase III, AgCMCase, alkali cellulase, Alkaline cellulase, AnCel5A, AtCel5, ATEG_07420, avicelase, BC-EG70a, Bc22Cel, BCE1, BcsZ, beta-1,4-endoglucan hydrolase, beta-1,4-endoglucanase, beta-1,4-glucanase, Bgl7A, bifunctional endoglucanase/xylanase, BlCel9, BP_Cel9A, Bx-ENG-1, Bx-ENG-2, Bx-ENG-3, C4endoII, carbomethyl cellulase, Carboxymethyl cellulase, Carboxymethyl-cellulase, carboxymethylcellulase, Cat 1, Caylase, CBH45-1, cbh6A, CBHI, CBHII, CcCel6C, CEL1, Cel1 EGase, Cel12A, Cel1753, Cel28a, Cel44A, Cel45A, Cel48A, Cel5, Cel5A, cel5B, Cel5E, Cel6A, Cel6A (E2), Cel6B, Cel6C, CEL7, Cel7A, Cel7B, Cel8, Cel8A, Cel8M, Cel8Y, Cel9A, CEL9A-50, CEL9A-65, Cel9A-68, CEL9A-82, Cel9A-90, Cel9B, CEL9C1, Cel9K, Cel9M, Cel9Q, CelA, CelB, CelC2 cellulase, CelCM3, CelDR, CelE, CelF, Celf_1230, Celf_3184, CelG, CelG endoglucanase, CelI15, cell-bound bacterial cellulase, CelL15, CelL73, cellic Ctec2, cellobiohydrolase, cellobiohydrolase I, celluase A, Celluclast, celludextrinase, Cellulase, cellulase 12A, cellulase A, cellulase A 3, cellulase Cel48F, cellulase Cel9A, cellulase Cel9M, cellulase CelC2, cellulase CelE, cellulase CM3, Cellulase E1, Cellulase E2, Cellulase E4, Cellulase E5, cellulase EGX, cellulase II, cellulase III, cellulase K, Cellulase SS, cellulase T, Cellulase V1, cellulase Xf818, cellulases I, cellulases III, cellulosin AP, Cellulysin, CelP, celS, CelStrep, celVA, CelX, CenA, CenC, CfCel6A, CfCel6C, CfEG3a, CHU_1280, CHU_2103, CjCel9A, Clocel_2741, CMCase, CMCase-I, CMCax, CMcellulase, Csac_1076, Csac_1078, CSCMCase, ctCel9D-Cel44A, CTendo45, ctendo7, CtGH5, Cthe_0435, CTHT_0045780, CX-cellulase, CyPB, DCC85_10145, DK-85, Dockerin type 1, Dtur_0671, E1 endoglucanase, Econase, EfPh, EG I, EG III, EG1, EG12, EG2, EG25, EG271, EG28, EG3, EG35, EG44, EG47, EG51, Eg5a, EG60, EGA, EGase, EGase II, EGB, EGC, EGCCA, EGCCC, EGCCD, EGCCF, EGCCG, EGD, EGE, EGF, egGH45, EGH, EGI, EGII, EGII/Cel5A, EGIV, EGL, EGL 1, Egl-257, Egl1, Egl499, Egl5a, EglA, eglB, EglC, EGLII, EglS, EGM, EGPf, EGPh, EGSS, EgV, EGX, EGY, EGZ, endo-1,4-B-glucanase, endo-1,4-beta-D-glucanase, endo-1,4-beta-glucanase, endo-1,4-beta-glucanase 1, endo-1,4-beta-glucanase 2, endo-1,4-beta-glucanase E1, endo-1,4-beta-glucanase V1, endo-beta-1,3-1,4-glucanase, endo-beta-1,4-glucanase, endo-beta-1,4-glucanase 1, endo-beta-1,4-glucanase 2, endo-beta-1,4-glucanase CMCax, endo-beta-1,4-glucanase EG27, endo-beta-1,4-glucanase EG45, endo-beta-D-1,4-glucanohydrolase, endo-beta-glucanase, endo-glucanase, ENDO1, ENDO2, endocellulase, endocellulase E1, endocellulases I, endocellulases II, endocellulases III, endocellulases IV, endogenous beta-1,4-endoglucanase, endogenous cellulase, endoglucanase, endoglucanase 1, endoglucanase 35, endoglucanase 47, endoglucanase CBP105, endoglucanase Cel 12A, endoglucanase Cel 5A, endoglucanase Cel 7B, endoglucanase Cel5A, endoglucanase Cel6A, endoglucanase D, endoglucanase EG-I, endoglucanase EG25, endoglucanase EG28, endoglucanase EG44, endoglucanase EG47, endoglucanase EG51, endoglucanase EG60, endoglucanase H, endoglucanase II, endoglucanase IIa, endoglucanase IV, endoglucanase L, endoglucanase M, endoglucanase V, endoglucanase Y, endolytic cellulase, EngA, EngH, EngL, EngM, engXCA, EngY, EngZ, family 7 cellobiohydrolase, FI-CMCASE, FnCel5A, FpCel45, Fpcel45a, GE40, GE40 endoglucanase, GH12 endo-1,4-beta-glucanase, GH124 endoglucanase, GH45 endoglucanase, gh45-1, GH5 cellulase, GH5 endoglucanase, GH6 endoglucanase, GH7 endoglucanase, GH9 termite cellulase, Glu1, Glu2, glycoside hydrolase family 9 endoglucanase, GtGH45, Lp-egl-1, manganese dependent endoglucanase, Maxazyme, Meicelase, mesophilic endoglucanase, mgCel6A, More, MtGH45, nmGH45, Onozuka R10, pancellase SS, PaPopCel1, PF0854, PH1171, PttCel9A, RCE1, RCE2, Roth 3056, RtGH124, Rucel5B, Sl-cel7, Sl-cel9C1, SnEG54, SoCel5, ssgluc, SSO1354, SSO1354 enzyme, SSO1354 protein, SSO1949, SSO2534, STCE1, Sumizyme, T12-GE40, TC Serva, TeEG-I, TeEgl5A, TfCel9A, ThEG, theme C glycoside hydrolase family 9 endo-beta-glucanase, Thermoactive cellulase, thermostable carboxymethyl cellulase, THITE_2110957, TM_1525, TM_1751, umcel5G, umcel9y-1, Vul_Cel5A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.4 cellulase

Inhibitors

Inhibitors on EC 3.2.1.4 - cellulase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-butyl-3-methylimidazolium chloride
92.8% and 43.8% activity remaining in 10% and 20% 1-butyl-3-methylimidazolium chloride
1-butyl-3-methylimidazolium methylsulfate
-
the enzyme precipitates immediately on addition to the reaction mixture
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
100 mM, 98% inhibition
2,4-dinitrophenol
-
-
2-mercaptoethanol
4-chloromercuribenzoate
-
5 mM, 90% of initial activity
4-hydroxybenzoic acid
-
-
acetonitril
-
11.4% inhibition at 5%
acetonitrile
-
10% v/v, 89% loss of activity
Al3+
Thermochaetoides thermophila
slight inhibition at 10 mM
alpha-chymotrypsin
-
denaturant
-
benzol
-
96.2% inhibition at 5%
butanol
-
10.9% inhibition at 5%
CaCl2
-
5 mM, 52% residual activity
carbodiimide
-
-
carboxymethylcellulose
-
binding of the cellulose-binding domain to avicel is inhibited by carboxymethylcellulose, but not by barley beta-glucan and glucose at concentration of 0.1% and 0.5%
-
cellobiose
cellotriose
-
-
cetyltrimethylammonium bromide
1 mM, 31% residual activity
CoCl2
-
5 mM, 35% loss of activity
Cr2+
-
1 mM, 27% residual activity
CuCl2
-
82% inhibition
CuSO4
-
5 mM, 38% residual activity
D-gluconic acid lactone
-
strong inhibition of membrane-bound cellulase
D-glucono-1,5-lactone
-
Dextran
-
partial inhibition of membrane-bound cellulase
diethyl dicarbonate
-
10 mM, 17.6% residual activity
diethyldicarbonate
-
-
Dimethylsulfoxide
-
30% v/v, 50% inhibition
dithiothreitol
DMSO
moderate inhibition
EGTA
10 mM, 24% inhibition
ethanol
FeCl2
-
5 mM, 38% residual activity
glucosamine
-
inhibition of CM-cellulase activity of enzyme E1, E3a, E3b and E4
glucose
Glutaraldehyde
-
-
glutathione
-
slight
guanidinium hydrochloride
72.2% at 3%
HgCl2
iodoacetamide
iodoacetic acid
-
-
Isopropanol
KMnO4
-
0.1 mM, complete inhibition of cellulase II-A
lactose
-
51000 Da subunit from the multicomponent cellulase complexes
lignin
-
at 45°C, lignin derived from acid hydrolyzed liquid hot water pretreated bagasse completely adsorbes cellulolytic enzymes from Trichoderma reesei within 90 min, while lignin derived from enzyme hydrolyzed liquid hot water pretreated bagasse adsorbes only 60% of Trichoderma reesei endoglucanase, exoglucanase and beta-glucosidase activities, overview. At 30°C, adsorption of all of the enzymes is minimal and enzyme hydrolysis at 30°C approaches that at 45°C after 168 h. Simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP), both carried out at 30-32°C, offer viable options for mitigating lignin-derived inhibition effects. Method overview. Specific surface area and surface characteristics of lignocellulose particles
-
linear alkyl benzene sulfonate
LAS
mercaptoethanol
methanol
methylcellulose
-
poor inhibitor of carboxymethylcellulase activity of enzyme form E3a, E3b and E4
-
MgCl2
-
5 mM, 52% residual activity
MnCl2
-
5 mM, 67% loss of activity
Monoiodoacetic acid
1 mM, 10% inhibition
N-bromosuccinimide
NaN3
0.1%, 45.2% inhibition of activity with carboxymethyl cellulose, 12.25% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
NiCl2
o-phenanthroline
1 mM, 75% inhibition
p-chloromercuribenzoate
0.01 mM, 20% inhibition
Pb(CH3COO)2
-
34% inhibition at 1 mM
PbCl2
-
5 mM, 37% loss of activity
phenylmercuric ion
-
-
Phenylmethyl sulfonylfluoride
-
1 mM, 30% residual activity
phenylmethylsulfonyl fluoride
sodium dodecyl benzene sulfonate
-
-
sodium dodecylsulfate
sodium hypochlorite
sodium picryl sulfate
-
2 mM, cellulase I
SrCl2
-
5 mM, 36% loss of activity
starch
-
inhibits hydrolysis of glucan
syringaldehyde
-
-
Tannic acid
-
-
Teepol
-
detergent, 0.1%, 80% inhibition
-
trans-cinnamic acid
-
-
Tris
Tris almost completely suppresses CtCel9Q hydrolase activity, a Tris molecule is bound to three catalytic residues of CtCel9Q and occupies subsite -1 of the CtCel9Q active-site cleft, enzyme mutant crystal structure analysis, overview
Triton X-100
Tween 20
31% inhibition at 20%
Tween-20
-
1%, 79% residual activity
ZnCl2
-
47% inhibition
additional information
-