Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, 174% of initial activity | Rasamsonia emersonii | |
sodium dodecylsulfate | 5 mM, 110% of initial activity | Rasamsonia emersonii |
Application | Comment | Organism |
---|---|---|
degradation | under simulated mashing conditions, addition of 60 U Egl5A reduces more viscosity (10.0 vs.7.6%) than 80 U of Ultraflo XL from Novozymes | Rasamsonia emersonii |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Rasamsonia emersonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | 5 mM, 34% residual activity | Rasamsonia emersonii | |
Cu2+ | 5 mM, 41% residual activity | Rasamsonia emersonii | |
EDTA | 5 mM, 46% residual activity | Rasamsonia emersonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Barley beta-glucan | Km value 12.2 mg/l, pH 4.5, 90°C | Rasamsonia emersonii | |
additional information | - |
carboxymethyl cellulose | Km value 31.7 mg/l, pH 4.5, 90°C | Rasamsonia emersonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 128% of initial activity | Rasamsonia emersonii | |
K+ | 5 mM, 137% of initial activity | Rasamsonia emersonii | |
Li+ | 5 mM, 150% of initial activity | Rasamsonia emersonii | |
Zn2+ | 5 mM, 142% of initial activity | Rasamsonia emersonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36800 | - |
x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme | Rasamsonia emersonii |
37000 | - |
x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme | Rasamsonia emersonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | X4YXV3 | - |
- |
Rasamsonia emersonii CBS394.64 | X4YXV3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme migrates on SDS-PAGE with several bands, with molecular masses larger than its theoretical value. after Endo H-treatment, Egl5A migrates as a single band of 37000 Da | Rasamsonia emersonii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.3 | - |
substrate birchwood xylan, pH 4.5, 90°C | Rasamsonia emersonii |
176 | - |
substrate carob bean gum, pH 4.5, 90°C | Rasamsonia emersonii |
624 | - |
substrate carboxymethyl cellulose, pH 4.5, 90°C | Rasamsonia emersonii |
3413 | - |
substrate lichenan, pH 4.5, 90°C | Rasamsonia emersonii |
4059 | - |
substrate laminarin, pH 4.5, 90°C | Rasamsonia emersonii |
4254 | - |
substrate barley beta-glucan, pH 4.5, 90°C | Rasamsonia emersonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Rasamsonia emersonii | ? | - |
? | |
birchwood xylan + H2O | - |
Rasamsonia emersonii | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Rasamsonia emersonii | ? | - |
? | |
carob bean gum + H2O | - |
Rasamsonia emersonii | ? | - |
? | |
laminarin + H2O | - |
Rasamsonia emersonii | ? | - |
? | |
lichenan + H2O | - |
Rasamsonia emersonii | ? | - |
? | |
additional information | enzyme displays broad substrate specificity and exhibits high activity on substrates containing beta-1,4-glycosidic bonds and beta-1,3-glycosidic bonds such as barley beta-glucan, laminarin, lichenan, carboxymethyl cellulose, carob bean gum, and birchwood xylan | Rasamsonia emersonii | ? | - |
? | |
additional information | no substrates: avicel, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-cellobioside | Rasamsonia emersonii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36800, calculated, x * 37000, SDS-PAGE of deglycosylated enzyme | Rasamsonia emersonii |
Synonyms | Comment | Organism |
---|---|---|
Egl5a | - |
Rasamsonia emersonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Rasamsonia emersonii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 95 | more than 70% of maximum activity | Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
1 h, 50% residual activity | Rasamsonia emersonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51.6 | - |
carboxymethyl cellulose | pH 4.5, 90°C | Rasamsonia emersonii | |
311 | - |
Barley beta-glucan | pH 4.5, 90°C | Rasamsonia emersonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Rasamsonia emersonii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | more than 90% of maximum activity | Rasamsonia emersonii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 10 | 37°C, 1 h, more than than 80% of maximum activity | Rasamsonia emersonii |