General Stability | Organism |
---|---|
dilute solutions, at 4°C, stable for several days | Thermoascus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
cellulase III, gel filtration | Thermoascus aurantiacus |
48000 | - |
cellulase II, gel filtration | Thermoascus aurantiacus |
78000 | - |
cellulase I, gel filtration | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | cellulase I contains 5.5% carbohydrate, cellulase II contains 2.6% carbohydrate, cellulase III contains 1.8% carbohydrate | Thermoascus aurantiacus |
Purification (Comment) | Organism |
---|---|
cellulase I, II and III | Thermoascus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermoascus aurantiacus |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 1 year | Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | hydrolyzed by cellulase I and III, no hydrolysis with cellulase II | Thermoascus aurantiacus | additional information | - |
? | |
carboxymethylpachyman + H2O | cellulase I | Thermoascus aurantiacus | ? | - |
? | |
cellulose + H2O | partial degradation of native cellulose | Thermoascus aurantiacus | ? | - |
? | |
glucan + H2O | yeast glucan | Thermoascus aurantiacus | ? | - |
? | |
laminarin + H2O | cellulase I | Thermoascus aurantiacus | ? | - |
? | |
lichenan + H2O | cellulase I, II and III | Thermoascus aurantiacus | ? | - |
? | |
additional information | hydrolysis of filter paper | Thermoascus aurantiacus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, cellulase III, SDS-PAGE | Thermoascus aurantiacus |
monomer | 1 * 48000, cellulase II, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
hydrolysis of yeast glucan with cellulase I or hydrolysis of carboxymethylcellulose with cellulase III | Thermoascus aurantiacus |
75 | - |
hydrolysis of carboxymethylcellulose with cellulase I | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable up to | Thermoascus aurantiacus |
75 | - |
less than 10% of the cellulolytic ativities remain after 1 h | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | cellulase I, II and III | Thermoascus aurantiacus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 12 | cellulase II is stable | Thermoascus aurantiacus |
5 | 9 | cellulase I and III are stable | Thermoascus aurantiacus |