Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Bursaphelenchus xylophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
gel filtration | Bursaphelenchus xylophilus |
18000 | - |
SDS-PAGE | Bursaphelenchus xylophilus |
25000 | - |
Bx-ENG-1 shows a single band with a molecular size of 25 kDa when 2-mercaptethanol is added to the SDS-PAGE sample | Bursaphelenchus xylophilus |
25380 | - |
calculated | Bursaphelenchus xylophilus |
30290 | - |
calculated | Bursaphelenchus xylophilus |
33000 | 39000 | SDS-PAGE | Bursaphelenchus xylophilus |
36440 | - |
calculated | Bursaphelenchus xylophilus |
67000 | - |
gel filtration | Bursaphelenchus xylophilus |
100000 | 140000 | SDS-PAGE | Bursaphelenchus xylophilus |
252000 | - |
gel filtration | Bursaphelenchus xylophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bursaphelenchus xylophilus | Q6BCL3 | - |
- |
Bursaphelenchus xylophilus | Q6BCL7 | - |
- |
Bursaphelenchus xylophilus | Q6BCM1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bursaphelenchus xylophilus |
no glycoprotein | - |
Bursaphelenchus xylophilus |
Purification (Comment) | Organism |
---|---|
- |
Bursaphelenchus xylophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amorphous cellulose + H2O | - |
Bursaphelenchus xylophilus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Bursaphelenchus xylophilus | ? | - |
? | |
glucomannan + H2O | - |
Bursaphelenchus xylophilus | ? | - |
? | |
lichenan + H2O | - |
Bursaphelenchus xylophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | Bx-ENG-2 exists in a glycosylated dimeric form | Bursaphelenchus xylophilus |
dimer | Bx-ENG-3 exists in a glycosylated dimeric form | Bursaphelenchus xylophilus |
monomer | Bx-ENG-1 exists in an unglycosylated monomeric form | Bursaphelenchus xylophilus |
Synonyms | Comment | Organism |
---|---|---|
Bx-ENG-1 | - |
Bursaphelenchus xylophilus |
Bx-ENG-2 | - |
Bursaphelenchus xylophilus |
Bx-ENG-3 | - |
Bursaphelenchus xylophilus |
endoglucanase | - |
Bursaphelenchus xylophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bursaphelenchus xylophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
more than 80% of maximal activity is observed for Bx-ENG-3 at 70°C | Bursaphelenchus xylophilus |
60 | 70 | more than 80% of maximal activity is observed for Bx-ENG-2 at 70°C | Bursaphelenchus xylophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Bursaphelenchus xylophilus |
6.4 | - |
- |
Bursaphelenchus xylophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 8.3 | the maximum activity range is broad, with 70% of maximum activity occurring between pH 4.2 and 8.3 | Bursaphelenchus xylophilus |