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Literature summary for 3.2.1.4 extracted from

  • Shibuya, H.; Kikuchi, T.
    Purification and characterization of recombinant endoglucanases from the pine wood nematode Bursaphelenchus xylophilus (2008), Biosci. Biotechnol. Biochem., 72, 1325-1332.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Bursaphelenchus xylophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
gel filtration Bursaphelenchus xylophilus
18000
-
SDS-PAGE Bursaphelenchus xylophilus
25000
-
Bx-ENG-1 shows a single band with a molecular size of 25 kDa when 2-mercaptethanol is added to the SDS-PAGE sample Bursaphelenchus xylophilus
25380
-
calculated Bursaphelenchus xylophilus
30290
-
calculated Bursaphelenchus xylophilus
33000 39000 SDS-PAGE Bursaphelenchus xylophilus
36440
-
calculated Bursaphelenchus xylophilus
67000
-
gel filtration Bursaphelenchus xylophilus
100000 140000 SDS-PAGE Bursaphelenchus xylophilus
252000
-
gel filtration Bursaphelenchus xylophilus

Organism

Organism UniProt Comment Textmining
Bursaphelenchus xylophilus Q6BCL3
-
-
Bursaphelenchus xylophilus Q6BCL7
-
-
Bursaphelenchus xylophilus Q6BCM1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Bursaphelenchus xylophilus
no glycoprotein
-
Bursaphelenchus xylophilus

Purification (Commentary)

Purification (Comment) Organism
-
Bursaphelenchus xylophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amorphous cellulose + H2O
-
Bursaphelenchus xylophilus ?
-
?
carboxymethyl cellulose + H2O
-
Bursaphelenchus xylophilus ?
-
?
glucomannan + H2O
-
Bursaphelenchus xylophilus ?
-
?
lichenan + H2O
-
Bursaphelenchus xylophilus ?
-
?

Subunits

Subunits Comment Organism
dimer Bx-ENG-2 exists in a glycosylated dimeric form Bursaphelenchus xylophilus
dimer Bx-ENG-3 exists in a glycosylated dimeric form Bursaphelenchus xylophilus
monomer Bx-ENG-1 exists in an unglycosylated monomeric form Bursaphelenchus xylophilus

Synonyms

Synonyms Comment Organism
Bx-ENG-1
-
Bursaphelenchus xylophilus
Bx-ENG-2
-
Bursaphelenchus xylophilus
Bx-ENG-3
-
Bursaphelenchus xylophilus
endoglucanase
-
Bursaphelenchus xylophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bursaphelenchus xylophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
more than 80% of maximal activity is observed for Bx-ENG-3 at 70°C Bursaphelenchus xylophilus
60 70 more than 80% of maximal activity is observed for Bx-ENG-2 at 70°C Bursaphelenchus xylophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
Bursaphelenchus xylophilus
6.4
-
-
Bursaphelenchus xylophilus

pH Range

pH Minimum pH Maximum Comment Organism
4.2 8.3 the maximum activity range is broad, with 70% of maximum activity occurring between pH 4.2 and 8.3 Bursaphelenchus xylophilus