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3.2.1.4: cellulase

This is an abbreviated version!
For detailed information about cellulase, go to the full flat file.

Word Map on EC 3.2.1.4

Reaction

cellohexaose
+
H2O
= 2 cellotriose

Synonyms

(1->4)-beta-D-glucan 4-glucanohydrolase, 1,4-beta-D-endoglucanase, 1,4-beta-D-glucan-4-glucanohydrolase, 168cel5, 9.5 cellulase, Abscission cellulase, AEG, Ag-EGase III, AgCMCase, alkali cellulase, Alkaline cellulase, AnCel5A, AtCel5, ATEG_07420, avicelase, BC-EG70a, Bc22Cel, BCE1, BcsZ, beta-1,4-endoglucan hydrolase, beta-1,4-endoglucanase, beta-1,4-glucanase, Bgl7A, bifunctional endoglucanase/xylanase, BlCel9, BP_Cel9A, Bx-ENG-1, Bx-ENG-2, Bx-ENG-3, C4endoII, carbomethyl cellulase, Carboxymethyl cellulase, Carboxymethyl-cellulase, carboxymethylcellulase, Cat 1, Caylase, CBH45-1, cbh6A, CBHI, CBHII, CcCel6C, CEL1, Cel1 EGase, Cel12A, Cel1753, Cel28a, Cel44A, Cel45A, Cel48A, Cel5, Cel5A, cel5B, Cel5E, Cel6A, Cel6A (E2), Cel6B, Cel6C, CEL7, Cel7A, Cel7B, Cel8, Cel8A, Cel8M, Cel8Y, Cel9A, CEL9A-50, CEL9A-65, Cel9A-68, CEL9A-82, Cel9A-90, Cel9B, CEL9C1, Cel9K, Cel9M, Cel9Q, CelA, CelB, CelC2 cellulase, CelCM3, CelDR, CelE, CelF, Celf_1230, Celf_3184, CelG, CelG endoglucanase, CelI15, cell-bound bacterial cellulase, CelL15, CelL73, cellic Ctec2, cellobiohydrolase, cellobiohydrolase I, celluase A, Celluclast, celludextrinase, Cellulase, cellulase 12A, cellulase A, cellulase A 3, cellulase Cel48F, cellulase Cel9A, cellulase Cel9M, cellulase CelC2, cellulase CelE, cellulase CM3, Cellulase E1, Cellulase E2, Cellulase E4, Cellulase E5, cellulase EGX, cellulase II, cellulase III, cellulase K, Cellulase SS, cellulase T, Cellulase V1, cellulase Xf818, cellulases I, cellulases III, cellulosin AP, Cellulysin, CelP, celS, CelStrep, celVA, CelX, CenA, CenC, CfCel6A, CfCel6C, CfEG3a, CHU_1280, CHU_2103, CjCel9A, Clocel_2741, CMCase, CMCase-I, CMCax, CMcellulase, Csac_1076, Csac_1078, CSCMCase, ctCel9D-Cel44A, CTendo45, ctendo7, CtGH5, Cthe_0435, CTHT_0045780, CX-cellulase, CyPB, DCC85_10145, DK-85, Dockerin type 1, Dtur_0671, E1 endoglucanase, Econase, EfPh, EG I, EG III, EG1, EG12, EG2, EG25, EG271, EG28, EG3, EG35, EG44, EG47, EG51, Eg5a, EG60, EGA, EGase, EGase II, EGB, EGC, EGCCA, EGCCC, EGCCD, EGCCF, EGCCG, EGD, EGE, EGF, egGH45, EGH, EGI, EGII, EGII/Cel5A, EGIV, EGL, EGL 1, Egl-257, Egl1, Egl499, Egl5a, EglA, eglB, EglC, EGLII, EglS, EGM, EGPf, EGPh, EGSS, EgV, EGX, EGY, EGZ, endo-1,4-B-glucanase, endo-1,4-beta-D-glucanase, endo-1,4-beta-glucanase, endo-1,4-beta-glucanase 1, endo-1,4-beta-glucanase 2, endo-1,4-beta-glucanase E1, endo-1,4-beta-glucanase V1, endo-beta-1,3-1,4-glucanase, endo-beta-1,4-glucanase, endo-beta-1,4-glucanase 1, endo-beta-1,4-glucanase 2, endo-beta-1,4-glucanase CMCax, endo-beta-1,4-glucanase EG27, endo-beta-1,4-glucanase EG45, endo-beta-D-1,4-glucanohydrolase, endo-beta-glucanase, endo-glucanase, ENDO1, ENDO2, endocellulase, endocellulase E1, endocellulases I, endocellulases II, endocellulases III, endocellulases IV, endogenous beta-1,4-endoglucanase, endogenous cellulase, endoglucanase, endoglucanase 1, endoglucanase 35, endoglucanase 47, endoglucanase CBP105, endoglucanase Cel 12A, endoglucanase Cel 5A, endoglucanase Cel 7B, endoglucanase Cel5A, endoglucanase Cel6A, endoglucanase D, endoglucanase EG-I, endoglucanase EG25, endoglucanase EG28, endoglucanase EG44, endoglucanase EG47, endoglucanase EG51, endoglucanase EG60, endoglucanase H, endoglucanase II, endoglucanase IIa, endoglucanase IV, endoglucanase L, endoglucanase M, endoglucanase V, endoglucanase Y, endolytic cellulase, EngA, EngH, EngL, EngM, engXCA, EngY, EngZ, family 7 cellobiohydrolase, FI-CMCASE, FnCel5A, FpCel45, Fpcel45a, GE40, GE40 endoglucanase, GH12 endo-1,4-beta-glucanase, GH124 endoglucanase, GH45 endoglucanase, gh45-1, GH5 cellulase, GH5 endoglucanase, GH6 endoglucanase, GH7 endoglucanase, GH9 termite cellulase, Glu1, Glu2, glycoside hydrolase family 9 endoglucanase, GtGH45, Lp-egl-1, manganese dependent endoglucanase, Maxazyme, Meicelase, mesophilic endoglucanase, mgCel6A, More, MtGH45, nmGH45, Onozuka R10, pancellase SS, PaPopCel1, PF0854, PH1171, PttCel9A, RCE1, RCE2, Roth 3056, RtGH124, Rucel5B, Sl-cel7, Sl-cel9C1, SnEG54, SoCel5, ssgluc, SSO1354, SSO1354 enzyme, SSO1354 protein, SSO1949, SSO2534, STCE1, Sumizyme, T12-GE40, TC Serva, TeEG-I, TeEgl5A, TfCel9A, ThEG, theme C glycoside hydrolase family 9 endo-beta-glucanase, Thermoactive cellulase, thermostable carboxymethyl cellulase, THITE_2110957, TM_1525, TM_1751, umcel5G, umcel9y-1, Vul_Cel5A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.4 cellulase

Purification

Purification on EC 3.2.1.4 - cellulase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1,4-beta-glucanase I and II
-
9.5 cellulase, acid cellulases, and membrane-bound cellulase
-
a chimeric xylanase/endoglucanase with an internal cellulase-binding domain by fusing the Bacillus subtilis xyn gene fragment to the 5'-end of the Cellulomonas fimi cenA, expressed in Escherichia coli
-
affinity chromatography on Ni-NTA column
-
ammonium sulfate precipitation and various column chromatography on fast protein liquid chromatography system to homogeneity
-
by Ni-NTA column purification and pretreatment by heat denaturation to remove accompanying proteins
-
catalytic core domain
-
cellulase 4.5 and cellulase 4.8
-
cellulase A and B
-
cellulase A, B and C
-
cellulase I and II
cellulase I, II and III
-
cellulase II-A and II-B
-
cellulase III
-
centrifuged at 12,000 x g for 10 min to remove cell depris, hot shock, ultrafiltration, dialysis, gel filtration
CMCase I
-
combination of ammonium sulfate precipitation, gel filtration, and ion exchange chromatographic techniques
-
deletion of the C-terminal region facilitates expression of soluble protein and has no effect on enzyme activity. Therefore the wild-type enzyme (389 amino acids) is expressed with both N (28 residues)- and C (42 residues)-terminal truncations
endo-beta-1,4-glucanase E1, E2 and E3
-
endoglucanase 35
-
endoglucanase 47
-
endoglucanase E1, E3a and E4
-
endoglucanase EG25
-
endoglucanase EG28
-
endoglucanase EG44
-
endoglucanase EG47
-
endoglucanase EG51
-
endoglucanase EG60
-
endoglucanase I
-
endoglucanase I and II
-
endoglucanase I, II, III and IV
-
endoglucanase I, II, III, IV, V and VI
-
endoglucanase III
-
EngH and truncated derivative
entire and truncated enzyme form
enzyme form E1, E2, E3, E4 and E5
-
exo-endo-1,4-beta-glucanase fusion protein
-
fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
fusion protein of glutathione-S-transferase and the catalytic domain of endoglucanase G
-
gel filtration
gene recombinant N-terminally His-tagged enzyme from Escherichia coli by ultracentrifugation at 100000 x g, nickel affinity chromatography, and ultrafiltration
hybrid cellulase with the Thermomonospora fusca E2 cellulose-binding domain at its C-terminus joined to the Prevotella ruminicola 40500 Da carboxymethylcellulase, expressed in Escherichia coli
-
isoenzyme T1, T2a, T2b, T3a, T3b
Sporotrichum pulverulentum
-
isoforms cellobiohydrolase I and cellobiohydrolase II
Thermochaetoides thermophila
-
isolation and separation of two different types of CMCases, type I and II, by anion exchange chromatography
-
multifunctional cellulase activity with endo-beta-1,4-glucanase activity, EC 3.2.1.4, exo-beta-1,4-glucanase activity, EC 3.2.9.11, and endo-beta-1,4-xylanase activity, EC 3.2.1.8
-
mutant enzyme DELTA1-90
native enzyme 29.5fold by anion exchange chromatography and gel filtration
-
native enzyme 3.7fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration, to homogeneity
-
native enzyme 53fold by anion exchange chromatography and gel filtration to homogeneity
-
native enzyme by ammonium sulfate fractionation and gel filtration
-
native enzyme from digestive fluid by gel filtration
native enzyme from fermentation medium by anion exchange chromatography, ultrafiltration and hydrophobic interaction chromatography followed by cleavage through papain to isolate the catalytic core domain, and gel filtration
-
native enzyme to homogeneity by ammonium sulfate fractionation and gel filtration
-
native extracellular enzyme 12.5fold from culture medium by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
-
Ni2+-chelating affinity column, SDS-PAGE
partially purified, ConA-Sepharose 4B affinity chromatography
phenyl-Sepharose column, followed by a Q-Sepharose column, assessed by SDS-PAGE
-
precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C
purified from bovine rumen
-
purified to near homogeneity, gel filtration
RCE1 and RCE2
-
recombinant Bgl7A 1.3fold from Pichia pastoris strain GS115 by ion exchange chromatography
recombinant enzyme
recombinant enzyme from Pichia pastoris strain GS115
recombinant form of Xf818 containing a His6 tag
recombinant His-tagged CcCel6C from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by TEV protease, again nickel affinity chromatography, and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS, cleavage of the His-tag by 3C protease
recombinant His-tagged enzyme from Escherichia coli strain C43 by nickel affinity chromatography
recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography
recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography to homogeneity
recombinant His-tagged Rucel5B from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged truncated mutant cellulase CtLic26A-Cel5 from Escherichia coli strain BL21 by metal affinity chromatography
-
recombinant N-terminally His-tagged enzyme 2535fold from Escherichia coli by nickel affinity chromatography and ultrafiltration
recombinant protein
recombinant protein expressed in Pichia pastoris and native protein
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 Codon Plus (DE3) RP
recominant His-tagged wild-type and mutant enzyme from Escherichia coli strain TOP10 by nickel affinity chromatography and ultrafiltration
-
the enzyme is the 51000 Da subunit from the multicomponent cellulase complexes
-
to homogeneity from the supernatant by ultrafiltration, DEAE-5PW anion exchange column and TSK-gel G3000SW gel filtration
two endo-beta-1,4-glucanase components: YEG1 and YEG2
-