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Literature summary for 3.2.1.4 extracted from
- Cellulases in Phaseolus vulgaris (1988), Methods Enzymol., 160, 342-351.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-gluconic acid lactone | strong inhibition of membrane-bound cellulase | Phaseolus vulgaris |  |
| Dextran | partial inhibition of membrane-bound cellulase | Phaseolus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound cellulase | Phaseolus vulgaris | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | Phaseolus vulgaris | in bean the 9.5 cellulase appears to function in the shedding of various organs such as fruits, flowers, and leaves. The acid cellulases function in loosening the cellulose fibrils of the cell wall to allow expansion and growth | cellooligosaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | membrane-bound cellulase | Phaseolus vulgaris |
| no modification | the 9.5 cellulase is not a glycoprotein | Phaseolus vulgaris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 9.5 cellulase, acid cellulases, and membrane-bound cellulase | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Phaseolus vulgaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Phaseolus vulgaris | additional information | - |
? | |
| cellulose + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
| cellulose + H2O | in bean the 9.5 cellulase appears to function in the shedding of various organs such as fruits, flowers, and leaves. The acid cellulases function in loosening the cellulose fibrils of the cell wall to allow expansion and growth | Phaseolus vulgaris | cellooligosaccharide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | acid cellulases | Phaseolus vulgaris |
| 5.1 | - |
membrane-bound cellulase | Phaseolus vulgaris |
| 8.1 | - |
9.5 cellulase, hydrolysis of cellulose | Phaseolus vulgaris |
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