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Literature summary for 3.2.1.4 extracted from
- Purification and properties of a lower-molecular-weight endo-cellulase from Irpex lacteus (Polyporus tulipiferae) (1980), J. Biochem., 87, 1625-1634.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.381 | - |
cellohexaose | - |
Irpex lacteus |  |
| 0.561 | - |
cellopentaose | - |
Irpex lacteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15500 | - |
gel filtration | Irpex lacteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Irpex lacteus | - |
i.e. Polyporus tulipiferae | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Irpex lacteus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.06 | - |
- |
Irpex lacteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Irpex lacteus | additional information | various cellooligosaccharides | ? | |
| cellohexaose + H2O | cellobiose is the main product | Irpex lacteus | cellobiose + cellotriose + cellotetraose + cellulose + cellopentaose | - |
? | |
| cellopentaose + H2O | cellobiose is the main product | Irpex lacteus | cellobiose + cellotriose + cellotetraose + cellulose | - |
? | |
| cellotetraose + H2O | - |
Irpex lacteus | 2 cellobiose | + glucose + cellotriose | ? | |
| additional information | no activity with cellobiose | Irpex lacteus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Irpex lacteus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 45% of maximal activity, 70°C: about 35% of maximal activity | Irpex lacteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, stable | Irpex lacteus |
| 50 | - |
10 min, about 50% loss of activity | Irpex lacteus |
| 70 | - |
10 min, almost complete inactivation | Irpex lacteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Irpex lacteus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7 | pH 2.0: about 60% of maximal activity, pH 7.0: about 40% of maximal activity | Irpex lacteus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | 30°C, 48 h, stable | Irpex lacteus |
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