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Literature summary for 3.2.1.4 extracted from

  • Leis, B.; Heinze, S.; Angelov, A.; Pham, V.T.; Thuermer, A.; Jebbar, M.; Golyshin, P.N.; Streit, W.R.; Daniel, R.; Liebl, W.
    Functional screening of hydrolytic activities reveals an extremely thermostable cellulase from a deep-sea archaeon (2015), Front. Bioeng. Biotechnol., 3, 95.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol improves enzymatic activity by 65.9-146.2% when added at final concentrations of 10 and 1.0 mM, respectively uncultured bacterium

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethyl cellulose Km-value is 2.35 mg/ml, pH 5.5, 92°C uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
CoCl2 0.5 mM, 210% of initial activity uncultured bacterium
KCl 500 mM, 75% increase in activity uncultured bacterium
MnCl2 1-2 mM, 188% of initial activity uncultured bacterium
NaCl 500 mM, 75% increase in activity uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 62300, calculated, x * 60000, SDS-PAGE uncultured bacterium
62300
-
 x * 62300, calculated, x * 60000, SDS-PAGE uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium A0A0F7YYA5 isolates of hyperthermophilic archaea from deep sea vents
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34
-
 substrate phosphoric acid-swollen cellulose, pH 5.5, 92°C uncultured bacterium
108
-
 substrate hydroxyethyl cellulose, pH 5.5, 92°C uncultured bacterium
272
-
 substrate lichenan, pH 5.5, 92°C uncultured bacterium
317
-
 substrate barley beta-glucan, pH 5.5, 92°C uncultured bacterium
692
-
 substrate carboxymethyl cellulose, pH 5.5, 92°C uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 uncultured bacterium ?
-
 ?
carboxymethyl cellulose + H2O
-
 uncultured bacterium ?
-
 ?
hydroxyethyl cellulose + H2O
-
 uncultured bacterium ?
-
 ?
lichenan + H2O
-
 uncultured bacterium ?
-
 ?
additional information enzyme displays highest specific activity against beta-1,4-glycosidic bonds of various linear glucan polysaccharides. Poor or no substrates: avicel, pachyman, laminarin, starch uncultured bacterium ?
-
 ?
phosphoric acid-swollen cellulose + H2O
-
 uncultured bacterium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62300, calculated, x * 60000, SDS-PAGE uncultured bacterium

Synonyms

Synonyms Comment Organism
Cel12A
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90 95
-
 uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 uncultured bacterium

pI Value

Organism Comment pI Value Maximum pI Value
uncultured bacterium calculated
-
 4.3