Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate | pH 1.8, 80°C | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Saccharolobus solfataricus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Saccharolobus solfataricus |
37508 | - |
x * 37508, calculated from sequence | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | Saccharolobus solfataricus | the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source | cellobiose + ? | - |
? | |
cellulose + H2O | Saccharolobus solfataricus P2 | the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source | cellobiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97VS7 | - |
- |
Saccharolobus solfataricus | Q97X08 | - |
- |
Saccharolobus solfataricus P2 | Q97VS7 | - |
- |
Saccharolobus solfataricus P2 | Q97X08 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
substrate: N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 1.8, 80°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Saccharolobus solfataricus | cellobiose + ? | - |
? | |
carboxymethylcellulose + H2O | - |
Saccharolobus solfataricus P2 | cellobiose + ? | - |
? | |
cellopentaose + H2O | - |
Saccharolobus solfataricus | cellobiose + cellotriose | - |
? | |
cellopentaose + H2O | - |
Saccharolobus solfataricus P2 | cellobiose + cellotriose | - |
? | |
cellotetraose + H2O | minimal substrate length, cellotriose and cellobiose are not hydrolyzed | Saccharolobus solfataricus | 2 cellobiose | - |
? | |
cellotetraose + H2O | minimal substrate length, cellotriose and cellobiose are not hydrolyzed | Saccharolobus solfataricus P2 | 2 cellobiose | - |
? | |
cellulose + H2O | the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source | Saccharolobus solfataricus | cellobiose + ? | - |
? | |
cellulose + H2O | the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source | Saccharolobus solfataricus P2 | cellobiose + ? | - |
? | |
additional information | the enzyme does not hydrolyse 4-nitrophenyl beta-D-cellobioside and 4-nitrophenyl beta-D-cellotrioside. No activity could be detected with crystalline cellulose at both acidic and neutral pH values | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme does not hydrolyse 4-nitrophenyl beta-D-cellobioside and 4-nitrophenyl beta-D-cellotrioside. No activity could be detected with crystalline cellulose at both acidic and neutral pH values | Saccharolobus solfataricus P2 | ? | - |
? | |
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O | - |
Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Saccharolobus solfataricus |
? | x * 37508, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SSO1949 | locus name | Saccharolobus solfataricus |
SSO2534 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 95 | 70°C: about 60% of maximal activity, 95°C: about 40% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 8 h at pH 1.8, 160 min at pH 1. At neutral pH, the thermal inactivation rate is nearly two orders of magnitude higher than at pH 1.8 | Saccharolobus solfataricus |
95 | - |
pH 1.8, rapid inactivation | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
1.8 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1.2 | 2.5 | pH 1.2: about 50% of maximal activity, pH 2-5: about 70% of maximal activity | Saccharolobus solfataricus |