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Literature summary for 3.2.1.4 extracted from

  • Wang, M.; Lai, G.L.; Nie, Y.; Geng, S.; Liu, L.; Zhu, B.; Shi, Z.; Wu, X.L.
    Synergistic function of four novel thermostable glycoside hydrolases from a long-term enriched thermophilic methanogenic digester (2015), Front. Microbiol., 6, 509.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Application

Application Comment Organism
degradation mixtures of beta-xylosidase, xylanase, beta-glucosidase, and cellulase isolated from the metagenomic library of a long-term dry thermophilic methanogenic digester community retain high residual synergistic activities after incubation with cellulose, xylan, and steam-exploded corncob at 50°C for 72 h. About 55% dry weight of steam-exploded corncob is hydrolyzed to glucose and xylose by the synergistic action of the four enzymes at 50°C for 48 h bacterium enrichment culture

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Avicel Km-value 19.5 g/l, pH 5.0, 50°C bacterium enrichment culture
additional information
-
 carboxymethyl cellulose Km-value 8.3 g/l, pH 5.0, 50°C bacterium enrichment culture

Organism

Organism UniProt Comment Textmining
bacterium enrichment culture A0A0H3V6E7 isolated from a metagenomic library of a long-term dry thermophilic methanogenic digester community
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bacterium enrichment culture A0A0H3V6E7 isolated from a thermophilic methanogenic digester community
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
174
-
 substrate carboxymethyl cellulose, pH 5.0, 50°C bacterium enrichment culture

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O 33.2% of the activtiy with carboxymethyl cellulose bacterium enrichment culture 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O 33.2% of the activity with carboxymethyl cellulose bacterium enrichment culture 4-nitrophenol + D-glucose
-
 ?
avicel + H2O 21.8% of the activity with carboxymethyl cellulose bacterium enrichment culture ?
-
 ?
avicel + H2O 21.8% of the activtiy with carboxymethyl cellulose bacterium enrichment culture ?
-
 ?
carboxymethyl cellulose + H2O
-
 bacterium enrichment culture ?
-
 ?
carboxymethyl cellulose + H2O
-
 bacterium enrichment culture ? products are mainly cello-oligosaccharides along with small amounts of glucose and cellobiose ?
filter paper + H2O 7.6% of the activity with carboxymethyl cellulose bacterium enrichment culture ?
-
 ?
filter paper + H2O 7.6% of the activtiy with carboxymethyl cellulose bacterium enrichment culture ?
-
 ?
xylan + H2O 3.3% of the activity with carboxymethyl cellulose bacterium enrichment culture ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel1753
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 bacterium enrichment culture

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37.2
-
 Avicel pH 5.0, 50°C bacterium enrichment culture
70.6
-
 carboxymethyl cellulose pH 5.0, 50°C bacterium enrichment culture