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Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
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Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
a conserved asparagine residue acts as a ligand to the catalytic water molecule, and another water molecule acts as a barrier during substrate recognition, slide-in mechanism for initial damage recognition, catalytic reaction mechanism, overview
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
active site structure and catalytic mechanism, overview
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Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
catalytic reaction mechanism, the active side comprises the GEGPG motif, residues 40-44, the side-chain of Arg161 in family 4 TthUDG might play a role in binding AP-DNA after catalysis
Q7WYV4
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
catalytic reaction mechanism, the catalytic residue is Asp93
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
the active site catalytic residue is Asp88
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Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
the UDG active center contorts the uracil and deoxyribose while the phosphates 5' and 3' of the uridine are anchored by interactions with a proline-rich loop and a glycine-serine loop, UDG catalytic mechanism with channeling of DNA repair intermediates, overview
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Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
uracil-DNA glycosylases are widespread enzymes that are found in all living organisms. UDGs form a central part of the DNA-repair machinery since they initiate the DNA base-excision repair pathway by hydrolysing the N-glycosidic bond between uracil and the deoxyribose sugar thereby catalysing the removal of mis-incorporated uracil from DNA
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
the active site catalytic residue is Asp88
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Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
active site structure and catalytic mechanism, overview
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Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
a conserved asparagine residue acts as a ligand to the catalytic water molecule, and another water molecule acts as a barrier during substrate recognition, slide-in mechanism for initial damage recognition, catalytic reaction mechanism, overview
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Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
catalytic reaction mechanism, the active side comprises the GEGPG motif, residues 40-44, the side-chain of Arg161 in family 4 TthUDG might play a role in binding AP-DNA after catalysis
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3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
3,N4-ethenocytosine + double-stranded DNA with abasic site
3,N4-ethenocytosine-mismatched single-stranded DNA + H2O
3,N4-ethenocytosine + single-stranded DNA with abasic site
Substrates: -
Products: -
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5-fluorouracil-mismatched double-stranded DNA + H2O
5-fluorouracil + double-stranded DNA with abasic site
5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
5-formyluracil-mismatched single-stranded DNA + H2O
5-formyluracil + single-stranded DNA with abasic site
Substrates: -
Products: -
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5-hydroxymethyl-uracil-mismatched double-stranded DNA + H2O
5-hydroxymethyl-uracil + double-stranded DNA with abasic site
Substrates: the preferred substrate of UDGb is hydroxymethyl-uracil mispaired with guanine, followed by G-U and A-U, UDGb is active on ethenocytosine-G and 5-fluorouracil-G pairs, and UDGb also performs processing of uracil and hydroxymethyluracil from single-stranded DNA, but highly prefers double-stranded DNA substrates
Products: -
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5-hydroxymethyl-uracil-mismatched single-stranded DNA + H2O
5-hydroxymethyl-uracil + single-stranded DNA with abasic site
Substrates: the preferred substrate of UDGb is hydroxymethyl-uracil mispaired with guanine, followed by G-U and A-U, UDGb is active on ethenocytosine-G and 5-fluorouracil-G pairs, and UDGb also performs processing of uracil and hydroxymethyluracil from single-stranded DNA, but highly prefers double-stranded DNA substrates
Products: -
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5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
5-hydroxyuracil-mismatched double-stranded DNA + H2O
5-hydroxyuracil + double-stranded DNA with abasic site
5-hydroxyuracil-mismatched single-stranded DNA + H2O
5-hydroxyuracil + single-stranded DNA with abasic site
5-methylcytosine-mismatched double-stranded DNA + H2O
5-methylcytosine + double-stranded DNA with abasic site
Substrates: -
Products: -
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8-oxoguanine-mismatched double-stranded DNA + H2O
8-oxoguanine + double-stranded DNA with abasic site
8-oxoguanine-mismatched single-stranded DNA + H2O
8-oxoguanine + single-stranded DNA with abasic site
deoxyuracil-containing single-stranded DNA + H2O
deoxyuracil + single-stranded DNA with abasic site
deoxyuracil-containing single-stranded RNA + H2O
deoxyuracil + single-stranded RNA
deoxyuracil-mismatched double-stranded DNA + H2O
deoxyuracil + double-stranded DNA with abasic site
double stranded DNA containing G-U mismatch + H2O
uracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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dUMP DNA + H2O
dUMP + DNA
dUMP-labeled calf thymus DNA + H2O
uracil + ?
ethenocytosine-mismatched double-stranded DNA + H2O
3,N4-ethenocytosine + double-stranded DNA with abasic site
Substrates: UDGb is active on ethenocytosine-G
Products: -
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fU-containing 10 nucleotide DNA sequence 5'-GGAGAfUCTCC-3' with opposing C, T, A, or G + H2O
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Substrates: -
Products: -
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hydroxymethyluracil-containing single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
hypoxanthine-mismatched double-stranded DNA + H2O
hypoxanthine + double-stranded DNA with abasic site
Substrates: the UDGb from Pyrobaculum aerophilum, belonging to a fifth UDG family, catalyzes the removal of uracil as well as of hypoxanthine from DNA by cleavage of e.g. hypoxanthine-thymine pairs, possessing an active site, that lacks the polar amino acid residue, see also EC 3.2.2.15, substrate specificity and active site structure, overview
Products: -
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M6-FAM-labeled single stranded oligonucleotide + H2O
uracil + ?
thymine-mismatched double-stranded DNA + H2O
thymine + double-stranded DNA with abasic site
uracil-containing calf thymus DNA + H2O
uracil + calf thymus DNA with abasic site
uracil-containing DNA + H2O
uracil + DNA with abasic site
uracil-containing double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
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Substrates: the rate of catalytic turnover (kcat), is higher with double-stranded substrate compared to single-stranded substrate (20/min versus 11/min), respectively. Catalytic efficiency is 3.5fold higher for single-stranded DNA due to the smaller Km-value
Products: -
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uracil-containing single stranded DNA + H2O
uracil + single stranded DNA with abasic site
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Substrates: duplex single stranded DNAs with sequences 5'-TGCACUUAAGAAUUTC-3'/5'-GAAATTCTTAAGTGCAGTGATAGTCTTCCGTCC-(CH2)7-methylene blue-3' and 5'-GAAATTCTTAAGTGCAGTGATAGTCTTCCGTCC-(CH2)7-methylene blue-3'/5'-TGGGGGTGCACTTAAGAATTTC-3'
Products: -
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uracil-containing single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
uracil-guanine-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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uracil-mismatched 13 nt bubbled double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
Substrates: low activity
Products: -
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uracil-mismatched 16 nt double-stranded DNA + H2O
uracil + 16 nt double-stranded DNA with abasic site
uracil-mismatched 19 nt bubbled double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
Substrates: second best substrate
Products: -
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uracil-mismatched 19 nt shift bubbled double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
Substrates: low activity
Products: -
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uracil-mismatched 7 nt bubbled double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
Substrates: low activity
Products: -
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uracil-mismatched DNA + H2O
uracil + DNA with abasic site
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
uracil-mismatched double-stranded DNA with A-U mismatch + H2O
uracil + double-stranded DNA with abasic site
uracil-mismatched double-stranded DNA with U-A mismatch + H2O
uracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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uracil-mismatched double-stranded DNA with U-G mismatch + H2O
uracil + double-stranded DNA with abasic site
uracil-mismatched double-stranded oligonucleotide + H2O
uracil + double-stranded oligonucleotide with abasic site
uracil-mismatched single-stranded DNA + H2O
uracil + single-stranded DNA with abasic site
uracil-mismatched single-stranded oligonucleotide + H2O
uracil + single-stranded oligonucleotide with abasic site
Substrates: -
Products: -
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xanthine-containing single-stranded DNA + H2O
xanthine + single-stranded DNA with abasic site
Substrates: -
Products: -
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additional information
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3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
3,N4-ethenocytosine + double-stranded DNA with abasic site
Substrates: -
Products: -
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3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
3,N4-ethenocytosine + double-stranded DNA with abasic site
Substrates: -
Products: -
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5-fluorouracil-mismatched double-stranded DNA + H2O
5-fluorouracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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5-fluorouracil-mismatched double-stranded DNA + H2O
5-fluorouracil + double-stranded DNA with abasic site
Substrates: UDGb is active on 5-fluorouracil-G pairs
Products: -
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5-fluorouracil-mismatched double-stranded DNA + H2O
5-fluorouracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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5-formyluracil-mismatched double-stranded DNA + H2O
5-formyluracil + double-stranded DNA with abasic site
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Substrates: -
Products: -
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5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
5-hydroxymethyluracil + double-stranded DNA with abasic site
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Substrates: -
Products: -
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5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
Substrates: -
Products: -
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5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
Substrates: -
Products: -
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5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
5-hydroxymethyluracil + single-stranded DNA with abasic site
Substrates: -
Products: -
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5-hydroxyuracil-mismatched double-stranded DNA + H2O
5-hydroxyuracil + double-stranded DNA with abasic site
Substrates: -
Products: -
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5-hydroxyuracil-mismatched double-stranded DNA + H2O
5-hydroxyuracil + double-stranded DNA with abasic site
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Substrates: -
Products: -
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5-hydroxyuracil-mismatched single-stranded DNA + H2O
5-hydroxyuracil + single-stranded DNA with abasic site
Substrates: DNA of Bacillus subtilis phage SPO1
Products: -
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