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Literature summary for 3.2.2.27 extracted from
- Structure determination of uracil-DNA N-glycosylase from Deinococcus radiodurans in complex with DNA (2015), Acta Crystallogr. Sect. D, 71, 2137-2149 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with DNA, vapor diffusion method, using 0.05 M sodium citrate pH 4.6, 20% (w/v) PEG 3350 | Deinococcus radiodurans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-containing single-stranded DNA + H2O | Deinococcus radiodurans | - |
uracil + single-stranded DNA with abasic site | - |
? |  |
| uracil-containing single-stranded DNA + H2O | Deinococcus radiodurans ATCC 13939 | - |
uracil + single-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Deinococcus radiodurans | - |
uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Deinococcus radiodurans ATCC 13939 | - |
uracil + double-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | Q9RWH9 | - |
- |
| Deinococcus radiodurans ATCC 13939 | Q9RWH9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Q Sepharose column chromatography | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-containing single-stranded DNA + H2O | - |
Deinococcus radiodurans | uracil + single-stranded DNA with abasic site | - |
? | |
| uracil-containing single-stranded DNA + H2O | - |
Deinococcus radiodurans ATCC 13939 | uracil + single-stranded DNA with abasic site | - |
? | |
| uracil-mismatched 16 nt double-stranded DNA + H2O | 5'-CCTGTCCAUGTCTCCG-3' | Deinococcus radiodurans | uracil + 16 nt double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched 16 nt double-stranded DNA + H2O | 5'-CCTGTCCAUGTCTCCG-3' | Deinococcus radiodurans ATCC 13939 | uracil + 16 nt double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Deinococcus radiodurans | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Deinococcus radiodurans ATCC 13939 | uracil + double-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DR_0968 | - |
Deinococcus radiodurans |
| UNG | - |
Deinococcus radiodurans |
| uracil-DNA N-glycosylase | - |
Deinococcus radiodurans |
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