Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N123D/L191A | the mutation generates an enzyme that excises cytosine and distinguishes between cytosine and methylcytosine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | Escherichia coli | - |
uracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P12295 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | - |
Escherichia coli | uracil + double-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDG | - |
Escherichia coli |
uracil DNA glycosylase | - |
Escherichia coli |