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Literature summary for 3.2.2.27 extracted from
- Purification and characterization of a cold-adapted uracil-DNA glycosylase from atlantic cod (Gadus morhua) (2000), Comp. Biochem. Physiol. B, 127, 399-410.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UNG inhibitor Ugi | from the Bacillus subtilis bacteriophage PBS2 | Gadus morhua |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | activates, optimally at 20-25 mM | Gadus morhua |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Gadus morhua |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | Gadus morhua | - |
uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | Gadus morhua | - |
uracil + single-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gadus morhua | - |
atlantic cod | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 17679fold from liver by anion and cation exchange chromatography, heparin and polyU affinity chromatography, and gel filtration | Gadus morhua |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gadus morhua | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 379 | - |
purified enzyme | Gadus morhua |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | - |
Gadus morhua | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | substrate specificity: the enzyme excises uracil bases from DNA, it has a 2fold higher activity for single-stranded DNA than for double-stranded DNA, the substrate dUMP DNA is prepared by nick-translation and PCR of single-stranded calf-thymus DNA | Gadus morhua | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | - |
Gadus morhua | uracil + single-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | substrate specificity: the enzyme excises uracil bases from DNA, it has a 2fold higher activity for single-stranded DNA than for double-stranded DNA, the substrate dUMP DNA is prepared by nick-translation and PCR of single-stranded calf-thymus DNA | Gadus morhua | uracil + single-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDG | - |
Gadus morhua |
| UNG | - |
Gadus morhua |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41 | - |
- |
Gadus morhua |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the UNG from Gadus morhua is cold-adapted and heat-labile | Gadus morhua |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the UNG from Gadus morhua is cold-adapted and heat-labile | Gadus morhua |
| 4 | - |
purified native enzyme, half-life is 2 h | Gadus morhua |
| 25 | - |
purified native enzyme, half-life is 20 min | Gadus morhua |
| 37 | - |
purified native enzyme, half-life is 6 min | Gadus morhua |
| 50 | - |
purified native enzyme, half-life is 0.5 min | Gadus morhua |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Gadus morhua |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | inactive at pH 10.0 | Gadus morhua |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | purified enzyme, 3% of maximal activity remaining at pH 5.5, most stable at pH 7.0-pH 9.5, 66% of maximal activity remaining at pH 10.0 | Gadus morhua |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Gadus morhua | above, isoelectric focusing | - |
9 |
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