Cloned (Comment) | Organism |
---|---|
gene ung-1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a Ung-1 defective mutant strain TM2862 shows no uracil excision activity, but the mutation in the ung-1 gene does not affect development, fertility and lifespan in Caenorhanditis elegans, suggesting the existence of backup enzyme | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bacillus subtilis Ung inhibitor | - |
Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, about, sequence calculation | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | Caenorhabditis elegans | - |
uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | Caenorhabditis elegans Bristol N2 | - |
uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Caenorhabditis elegans | - |
uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Caenorhabditis elegans Bristol N2 | - |
uracil + single-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Caenorhabditis elegans Bristol N2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Ung-1 from Escherichia coli strain BL21 | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UNG-1 removes uracil from both U-G and U-A base pairs in DNA. It also removes uracil from single-stranded oligonucleotide substrate less efficiently than double-stranded oligonucleotide. The active site A is present between residues 116-137, active site B at residues 247-253. Aromatic residues Tyr125, Phe136 and His247 are involved in the stacking interaction with uracil | Caenorhabditis elegans | ? | - |
? | |
additional information | UNG-1 removes uracil from both U-G and U-A base pairs in DNA. It also removes uracil from single-stranded oligonucleotide substrate less efficiently than double-stranded oligonucleotide. The active site A is present between residues 116-137, active site B at residues 247-253. Aromatic residues Tyr125, Phe136 and His247 are involved in the stacking interaction with uracil | Caenorhabditis elegans Bristol N2 | ? | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Caenorhabditis elegans | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Caenorhabditis elegans Bristol N2 | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Caenorhabditis elegans | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Caenorhabditis elegans Bristol N2 | uracil + single-stranded DNA with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, about, sequence calculation | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
More | UNG-1 belongs to the UNG family 1 | Caenorhabditis elegans |
UNG-1 | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Caenorhabditis elegans |