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Literature summary for 3.2.2.27 extracted from

  • Purnapatre, K.; Varshney, U.
    Uracil DNA glycosylase from Mycobacterium smegmatis and its distinct biochemical properties (1998), Eur. J. Biochem., 256, 580-588.
    View publication on PubMed
Search for more literature for 3.2.2.27

Application

Application Comment Organism
drug development the UDG from Mycobacterium smegmatis might be a useful target for inhibitor design Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
UGD inhibition protein from Bacillus subtilis phage PBS-2-encoded uracil DNA glycosylase inhibitor, UDG forms a dissociable, activity-reduced complex with the inhibitor protein Ugi in 1:1 molar stoichiometry Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycolicibacterium smegmatis UDG initiates uracil excision repair to safeguard the genomic integrity, mechanism, overview ?
-
 ?
additional information Mycolicibacterium smegmatis SN2 UDG initiates uracil excision repair to safeguard the genomic integrity, mechanism, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis SN2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 3111fold by anion exchange chromatography, gel filtration, another step of a different anion exchange chromatography, and DNA affinity and hydroxyapatatite chromatography Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14000
-
 purified native enzyme Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDG initiates uracil excision repair to safeguard the genomic integrity, mechanism, overview Mycolicibacterium smegmatis ?
-
 ?
additional information Mycobacterium smegmatis UDG excises uracil from different loop positions of tetraloop hairpin substrates with comparable efficiencies, substrate Escherichia coli RZ1032 dut1 ung1 genomic DNA Mycolicibacterium smegmatis ?
-
 ?
additional information UDG initiates uracil excision repair to safeguard the genomic integrity, mechanism, overview Mycolicibacterium smegmatis SN2 ?
-
 ?
additional information Mycobacterium smegmatis UDG excises uracil from different loop positions of tetraloop hairpin substrates with comparable efficiencies, substrate Escherichia coli RZ1032 dut1 ung1 genomic DNA Mycolicibacterium smegmatis SN2 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Mycolicibacterium smegmatis
More the initiating amino acid, formyl-methionine is cleaved from the mature UDG protein Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
uracil DNA glycosylase
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 first optimum Mycolicibacterium smegmatis
8.5 9 second optimum Mycolicibacterium smegmatis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5
-
 Mycolicibacterium smegmatis