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Literature summary for 3.2.2.27 extracted from
- The versatile thymine DNA-glycosylase: a comparative characterization of the human, Drosophila and fission yeast orthologs (2003), Nucleic Acids Res., 31, 2261-2271.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Thd1, phylogenetic analysis, functional expression of a His-tagged truncated variant comprising residues 650M-1063N in Escherichia coli strain BL21 (DE3) | Drosophila melanogaster |
| phylogenetic analysis, functional expression of Thp1p in Escherichia coli strain BL21 (DE3) | Schizosaccharomyces pombe |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a His-tagged truncated Thd1p variant comprising residues 650M-1063N from Escherichia coli strain BL21 (DE3) | Drosophila melanogaster |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
| 60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | Schizosaccharomyces pombe | - |
3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? |  |
| 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | Drosophila melanogaster | - |
3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
| 5-fluorouracil-mismatched double-stranded DNA + H2O | Schizosaccharomyces pombe | - |
5-fluorouracil + double-stranded DNA with abasic site | - |
? | |
| 5-fluorouracil-mismatched double-stranded DNA + H2O | Drosophila melanogaster | - |
5-fluorouracil + double-stranded DNA with abasic site | - |
? | |
| 5-methylcytosine-mismatched double-stranded DNA + H2O | Drosophila melanogaster | - |
5-methylcytosine + double-stranded DNA with abasic site | - |
? | |
| additional information | Drosophila melanogaster | 5-methylcytosine and thymine derivatives are processed with an appreciable efficiency only by the human and the Drosophila enzymes | ? | - |
? | |
| thymine-mismatched double-stranded DNA + H2O | Drosophila melanogaster | - |
thymine + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Schizosaccharomyces pombe | - |
uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Drosophila melanogaster | - |
uracil + double-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q9V4D8 | - |
- |
| Schizosaccharomyces pombe | O59825 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Thp1p from Escherichia coli strain BL21 (DE3) | Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
glycosylase activity of the recombinant enzyme, differential G-T processing, overview | Schizosaccharomyces pombe |
| additional information | - |
glycosylase activity of the recombinant enzyme, differential G-T processing, overview | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | - |
Schizosaccharomyces pombe | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
| 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
| 5-fluorouracil-mismatched double-stranded DNA + H2O | - |
Schizosaccharomyces pombe | 5-fluorouracil + double-stranded DNA with abasic site | - |
? | |
| 5-fluorouracil-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | 5-fluorouracil + double-stranded DNA with abasic site | - |
? | |
| 5-methylcytosine-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | 5-methylcytosine + double-stranded DNA with abasic site | - |
? | |
| additional information | 5-methylcytosine and thymine derivatives are processed with an appreciable efficiency only by the human and the Drosophila enzymes | Drosophila melanogaster | ? | - |
? | |
| additional information | the enzyme shows a broad and species-specific substrate spectrum, substrate binding structure, overview. The common most efficiently processed substrates of all are uracil and 3,N4-ethenocytosine opposite guanine and 5-fluorouracil in any double-stranded DNA context, the enzyme is able to hydrolyze a non-damaged 5'-methylcytosine opposite G, and the double strand and mismatch dependency of the enzymes varies with the substrate | Drosophila melanogaster | ? | - |
? | |
| additional information | the enzyme shows a broad and species-specific substrate spectrum, substrate binding structure, overview. The common most efficiently processed substrates of all are uracil and 3,N4-ethenocytosine opposite guanine and 5-fluorouracil in any double-stranded DNA context, the enzyme is able to hydrolyze a non-damaged 5'-methylcytosine opposite G, and the double strand and mismatch dependency of the enzymes varies with the substrate, G-T mismatch is no substrate for Thp1p. Thp1p shows little preference for mismatched substrate and processes uracil opposite A or in an ssDNA context with remarkable efficiency, overview | Schizosaccharomyces pombe | ? | - |
? | |
| thymine-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | thymine + double-stranded DNA with abasic site | - |
? | |
| thymine-mismatched double-stranded DNA + H2O | G-T mismatch is only a poor substrate for Thd1p | Drosophila melanogaster | thymine + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Schizosaccharomyces pombe | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | uracil + double-stranded DNA with abasic site | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
| ? | x * 60000, recombinant enzyme, SDS-PAGE | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Thd1p | - |
Drosophila melanogaster |
| Thp1p | - |
Schizosaccharomyces pombe |
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