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Literature summary for 3.2.2.27 extracted from

  • Kim, G.; Sun, Y.; Song, J.; Bae, H.; Kim, J.; Kwon, S.
    Properties of cold-active uracil-DNA glycosylase from Photobacterium aplysiae GMD509, and its PCR application for carryover contamination control (2009), Enzyme Microb. Technol., 44, 263-268.
No PubMed abstract available
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
UDG expression in Escherichia coli strain BL21(DE3) Photobacterium aplysiae

Organism

Organism UniProt Comment Textmining
Photobacterium aplysiae C1INF0
-
-
Photobacterium aplysiae GMD509 C1INF0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant UDG from Escherichia coli strain BL21(DE3) by heparin affinity chromatography and gel filtration Photobacterium aplysiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6697
-
 purified recombinant UDG Photobacterium aplysiae

Synonyms

Synonyms Comment Organism
UDG
-
 Photobacterium aplysiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 cold-active UDG Photobacterium aplysiae
25 30
-
 Photobacterium aplysiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 UDG, half-life is 77 s Photobacterium aplysiae
50
-
 UDG, half-life is 33 s Photobacterium aplysiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Photobacterium aplysiae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
 Photobacterium aplysiae