Literature summary for 3.2.2.27 extracted from

Assefa, N. G.; Niiranen, L.; Willassen, N. P.; Smalas, A.; Moe, E.
Thermal unfolding studies of cold adapted uracil-DNA N-glycosylase (UNG) from Atlantic cod (Gadus morhua). A comparative study with human UNG (2012), Comp. Biochem. Physiol. B, 161, 60-68 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli NR8052 cells	Gadus morhua
expressed in Escherichia coli NR8052 cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme K302R, hanging drop vapor diffusion method, using 0.1 M imidazole/maleate pH 7.8, 50 mM NaCl, 41 mM ammonium sulfate and 18% (w/v) PEG4000	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D183G	the mutant shows a slight increase in stability with concomitant reduction in the enzyme activity	Homo sapiens
D183G/K302R	the mutant shows a slight increase in stability with concomitant reduction in the enzyme activity	Homo sapiens
G183D	the mutation causes a reduction in the enzyme activity but no increase in stability	Gadus morhua
G183D/R302K	the mutations cause a reduction in the enzyme activity but no increase in stability	Gadus morhua

General Stability

General Stability	Organism
HEPES pH 7.5, Tris pH 7.5, and imidazole pH 8.0 are the most stabilizing buffers for the enzyme	Homo sapiens
Tris pH 7.5 and 8.0, and HEPES pH 7.5 are the most stabilizing buffers for the enzyme	Gadus morhua

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0004	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 37°C	Gadus morhua
0.0005	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 15°C	Gadus morhua
0.0006	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 22°C	Gadus morhua
0.00074	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 22°C	Gadus morhua
0.0008	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 37°C	Gadus morhua
0.0008	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 22°C	Gadus morhua
0.00083	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 37°C	Gadus morhua
0.001	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 15°C	Gadus morhua
0.00113	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 15°C	Gadus morhua
0.00157	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 37°C	Homo sapiens
0.0021	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 37°C	Homo sapiens
0.0022	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 15°C	Homo sapiens
0.00226	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 15°C	Homo sapiens
0.00238	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 15°C	Homo sapiens
0.0024	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 22°C	Homo sapiens
0.00265	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 37°C	Homo sapiens
0.00276	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 22°C	Homo sapiens
0.00277	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 22°C	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uracil-containing single-stranded DNA + H2O	Gadus morhua	-	uracil + single-stranded DNA with abasic site	-	?
uracil-containing single-stranded DNA + H2O	Homo sapiens	-	uracil + single-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	Gadus morhua	-	uracil + double-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	Homo sapiens	-	uracil + double-stranded DNA with abasic site	-	?

Organism

Organism	UniProt	Comment	Textmining
Gadus morhua	-	atlantic cod	-
Homo sapiens	P13051	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography	Gadus morhua
Q-Sepharose column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
uracil-containing calf thymus DNA + H2O	-	Gadus morhua	uracil + calf thymus DNA with abasic site	-	?
uracil-containing calf thymus DNA + H2O	-	Homo sapiens	uracil + calf thymus DNA with abasic site	-	?
uracil-containing single-stranded DNA + H2O	-	Gadus morhua	uracil + single-stranded DNA with abasic site	-	?
uracil-containing single-stranded DNA + H2O	-	Homo sapiens	uracil + single-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	-	Gadus morhua	uracil + double-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	-	Homo sapiens	uracil + double-stranded DNA with abasic site	-	?

Synonyms

Synonyms	Comment	Organism
UNG	-	Gadus morhua
UNG	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45.1	-	melting temperature	Gadus morhua
53.9	-	melting temperature	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.45	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 15°C	Homo sapiens
1.65	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 15°C	Homo sapiens
2	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 15°C	Gadus morhua
2.37	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 15°C	Homo sapiens
4.07	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 22°C	Homo sapiens
4.08	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 22°C	Gadus morhua
4.55	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 22°C	Homo sapiens
4.65	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 15°C	Gadus morhua
4.67	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 22°C	Gadus morhua
5.33	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 22°C	Homo sapiens
7.73	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 37°C	Homo sapiens
9.83	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 37°C	Gadus morhua
10.28	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 22°C	Gadus morhua
10.78	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 37°C	Homo sapiens
13.6	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 37°C	Gadus morhua
16.85	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 37°C	Homo sapiens
20.68	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 37°C	Gadus morhua
30.3	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 15°C	Gadus morhua

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00065	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 15°C	Homo sapiens
0.0007	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 15°C	Homo sapiens
0.0011	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 15°C	Homo sapiens
0.00147	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 22°C	Homo sapiens
0.00163	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 22°C	Homo sapiens
0.00178	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 15°C	Gadus morhua
0.00225	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 22°C	Homo sapiens
0.0032	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 15°C	Gadus morhua
0.0052	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 37°C	Homo sapiens
0.0055	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 22°C	Gadus morhua
0.0064	-	uracil-containing calf thymus DNA	mutant enzyme D183G/K302R, at pH 7.5 and 37°C	Homo sapiens
0.0078	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 22°C	Gadus morhua
0.01	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 15°C	Gadus morhua
0.012	-	uracil-containing calf thymus DNA	mutant enzyme G183D, at pH 7.5 and 37°C	Gadus morhua
0.013	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 22°C	Gadus morhua
0.025	-	uracil-containing calf thymus DNA	wild type enzyme, at pH 7.5 and 37°C	Gadus morhua
0.036	-	uracil-containing calf thymus DNA	mutant enzyme G183D/R302K, at pH 7.5 and 37°C	Gadus morhua
0.06	-	uracil-containing calf thymus DNA	mutant enzyme D183G, at pH 7.5 and 37°C	Homo sapiens

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Release 2023.1 (January 2023)