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Literature summary for 3.2.2.27 extracted from
- Thermostable uracil-DNA glycosylase from Thermotoga maritima, a member of a novel class of DNA repair enzymes (1999), Curr. Biol., 9, 531-534.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene udg, DNA and amino acid sequence determination, analysis and comparison, expression of His-tagged UDG in Escherichia coli strain BW310(DE3) | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | Thermotoga maritima | UDG is a DNA repair enzyme removing uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP, and it is the primary activity in the DNA base excision repair pathway, overview | uracil + double-stranded DNA with abasic site | - |
? |  |
| uracil-mismatched double-stranded DNA + H2O | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | UDG is a DNA repair enzyme removing uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP, and it is the primary activity in the DNA base excision repair pathway, overview | uracil + double-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
| Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged UDG from Escherichia coli strain BW310(DE3) by nickel affinity chromatography and gel filtration | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | UDG is a DNA repair enzyme removing uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP, and it is the primary activity in the DNA base excision repair pathway, overview | Thermotoga maritima | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | TMUDG removes uracil from double-stranded oligonucleotides containing either a U-G or a U-A base pair, e.g. from a 30mer ds oligonucleotide, DNA containing 3H-labeled uracil substrate preparation by nick translation of calf thymus DNA, overview | Thermotoga maritima | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | UDG is a DNA repair enzyme removing uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP, and it is the primary activity in the DNA base excision repair pathway, overview | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | TMUDG removes uracil from double-stranded oligonucleotides containing either a U-G or a U-A base pair, e.g. from a 30mer ds oligonucleotide, DNA containing 3H-labeled uracil substrate preparation by nick translation of calf thymus DNA, overview | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | uracil + double-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | Thermotoga maritima UDG is a member of one of three classes of uracil-DNA glycosylases | Thermotoga maritima |
| TMUDG | - |
Thermotoga maritima |
| UDG | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 75 | - |
Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Thermotoga maritima |
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Release 2023.1 (January 2023)
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