Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F98H | site-directed mutagenesis, the mutant shows reduced activity with uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil compared to the wild-type enzyme | Homo sapiens |
F98L | site-directed mutagenesis, the mutant shows reduced activity with uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil compared to the wild-type enzyme | Homo sapiens |
G87A | site-directed mutagenesis, the mutant shows reduced activity with uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil compared to the wild-type enzyme | Homo sapiens |
H239L | site-directed mutagenesis, the mutant shows reduced activity with uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil compared to the wild-type enzyme | Homo sapiens |
H239N | site-directed mutagenesis, the mutant shows reduced activity with uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil compared to the wild-type enzyme | Homo sapiens |
N163D | site-directed mutagenesis, the mutant shows reduced activity with uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil compared to the wild-type enzyme | Homo sapiens |
N85A | site-directed mutagenesis, the mutant shows reduced activity with uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of mutant enzymes, overview | Homo sapiens | |
0.0000022 | - |
uracil-mismatched double-stranded DNA with U-G mismatch | pH 7.5, 37°C, wild-type enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyluracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
5-formyluracil + double-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
5-hydroxyuracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
5-hydroxyuracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
uracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q53HV7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyluracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-formyluracil + double-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
5-hydroxyuracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxyuracil + double-stranded DNA with abasic site | - |
? | |
additional information | substrate specificity, the enzyme is not active with other oxidized pyrimidines such as 5-hydroxycytosine, 5-formylcytosine and thymine glycol, and intact pyrimidines such as thymine and cytosine. Mutational analysis of the catalytic and damage-recognition mechanism of hSMUG1, overview | Homo sapiens | ? | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA with U-G mismatch + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure and molecular modelling | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
single-strand selective monofunctional uracil-DNA glycosylase | - |
Homo sapiens |
SMUG1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
uracil-mismatched double-stranded DNA with U-G mismatch | pH 7.5, 37°C, wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |