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Literature summary for 3.2.2.27 extracted from
- Uracil-DNA glycosylase of Thermoplasma acidophilum directs long-patch base excision repair, which is promoted by deoxynucleoside triphosphates and ATP/ADP, into short-patch repair (2011), J. Bacteriol., 193, 4495-4508.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Thermoplasma acidophilum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25600 | - |
x * 25600, SDS-PAGE | Thermoplasma acidophilum |
| 25601 | - |
x * 25601, calculated from sequence | Thermoplasma acidophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | Q9HKW6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded oligonucleotide + H2O | - |
Thermoplasma acidophilum | uracil + double-stranded oligonucleotide with abasic site | - |
? |  |
| uracil-mismatched single-stranded oligonucleotide + H2O | - |
Thermoplasma acidophilum | uracil + single-stranded oligonucleotide with abasic site | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25600, SDS-PAGE | Thermoplasma acidophilum |
| ? | x * 25601, calculated from sequence | Thermoplasma acidophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ta0477 | - |
Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Thermoplasma acidophilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | excises 0.04 uracils per sec from uracil-mismatched oligonucleotide | Thermoplasma acidophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermoplasma acidophilum |
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Release 2023.1 (January 2023)
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