Application | Comment | Organism |
---|---|---|
molecular biology | UDG forms an apyrimidinic site, where an uracil base is removed, and facilitates the reactions of various DNA-repairing enzymes, such as AP endonuclease, DNA polymerase, and DNA ligase, it is thus useful in applications to remove uracil-DNA. Use of UDG to control carry-over contamination in PCR | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
Bsp HJ171 UDG gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of UDG containing an N-terminal intein cleavage site with an affinity chitin-binding tag under the control of a T7lac promoter, pTYB1 plasmid, in Escherichia coli strain BL21(DE3) | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activates optimal at 25 mM, recombinant UDG | Bacillus sp. (in: Bacteria) | |
NaCl | activates, optimal at 25 mM, recombinant UDG | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, recombinant detagged enzyme, SDS-PAGE, x * 27218, sequence calculation | Bacillus sp. (in: Bacteria) |
27218 | - |
x * 27000, recombinant detagged enzyme, SDS-PAGE, x * 27218, sequence calculation | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | B6CM51 | - |
- |
Bacillus sp. (in: Bacteria) HJ171 | B6CM51 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant UDG from Escherichia coli strain BL21(DE3) by chitin affinity chromatogaphy to homogeneity, the tag is cleaved off by intein | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UDG specifically and selectively removes uracil bases from DNA, substrate specificity, overview | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | UDG specifically and selectively removes uracil bases from DNA, substrate specificity, overview | Bacillus sp. (in: Bacteria) HJ171 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, recombinant detagged enzyme, SDS-PAGE, x * 27218, sequence calculation | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
UDG | - |
Bacillus sp. (in: Bacteria) |
UNG | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 25 | recombinant UDG | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is heat-labile and cold-active | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is heat-labile and cold-active | Bacillus sp. (in: Bacteria) |
30 | - |
rapid decrease of enzyme activity above 30°C | Bacillus sp. (in: Bacteria) |
40 | - |
half-life of the purified recombinant intein-tagged UDG is 131 s | Bacillus sp. (in: Bacteria) |
50 | - |
half-life of the purified recombinant intein-tagged UDG is 45 s | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant UDG | Bacillus sp. (in: Bacteria) |