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Literature summary for 3.2.2.27 extracted from

  • Wu, D.; Chen, L.; Sun, Q.; Wu, X.; Jia, S.; Meng, A.
    Uracil-DNA glycosylase is involved in DNA demethylation and required for embryonic development in the zebrafish embryo (2014), J. Biol. Chem., 289, 15463-15473.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Danio rerio

Protein Variants

Protein Variants Comment Organism
D132A inactive Danio rerio

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Danio rerio 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O Danio rerio
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Danio rerio

Source Tissue

Source Tissue Comment Organism Textmining
blastomere
-
 Danio rerio
-
egg
-
 Danio rerio
-
embryo
-
 Danio rerio
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O
-
 Danio rerio uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
UDG
-
 Danio rerio
Unga isoform Danio rerio
uracil-DNA glycosylase a isoform Danio rerio

General Information

General Information Comment Organism
malfunction enzyme knockdown in the zebrafish embryo increases global DNA methylation level, inhibits transcription, and causes embryonic lethality Danio rerio
physiological function uracil-DNA glycosylase is involved in postfertilization genomic DNA demethylation and zygotic gene transcription and is required for normal embryonic development in the zebrafish embryo. Enzyme-mediated DNA demethylation facilitates histone modifications of chromatin in zebrafish embryos Danio rerio