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Literature summary for 3.2.2.27 extracted from
- Ide, H.: Mammalian 5-formyluracil-DNA glycosylase. 2. Role of SMUG1 uracil-DNA glycosylase in repair of 5-formyluracil and other oxidized and deaminated base lesions (2003), Biochemistry, 42, 5003-5012.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| SMUG1 from kidney, expression of GST-tagged SMUG1 in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
| SMUG1 from liver cDNA library, expression of GST-tagged SMUG1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formyluracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
5-formyluracil + double-stranded DNA with abasic site | - |
? |  |
| 5-formyluracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
5-formyluracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | Rattus norvegicus | - |
5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxyuracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxyuracil-mismatched single-stranded DNA + H2O | Rattus norvegicus | - |
5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
| additional information | Homo sapiens | dual role of hSMUG1 as a backup enzyme for UNG and a primary repair enzyme for a subset of oxidized pyrimidines such as 5-formyluracil, 5-hydroxymethyluracil, and 5-hydroxyuracil | ? | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Rattus norvegicus | - |
uracil + double-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q53HV7 | - |
- |
| Rattus norvegicus | Q811Q1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged SMUG1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin | Homo sapiens |
| recombinant GST-tagged SMUG1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formyluracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-formyluracil + double-stranded DNA with abasic site | - |
? | |
| 5-formyluracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 5-formyluracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | - |
Rattus norvegicus | 5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxyuracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxyuracil + double-stranded DNA with abasic site | - |
? | |
| 5-hydroxyuracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
| 5-hydroxyuracil-mismatched single-stranded DNA + H2O | - |
Rattus norvegicus | 5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
| additional information | substrate specificity, overview | Rattus norvegicus | ? | - |
? | |
| additional information | dual role of hSMUG1 as a backup enzyme for UNG and a primary repair enzyme for a subset of oxidized pyrimidines such as 5-formyluracil, 5-hydroxymethyluracil, and 5-hydroxyuracil | Homo sapiens | ? | - |
? | |
| additional information | substrate specificity, overview. hSMUG1 removes damaged bases from Fenton-oxidized calf thymus DNA, generating abasic sites | Homo sapiens | ? | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Rattus norvegicus | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | uracil + single-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5-formyluracil-DNA glycosylase | - |
Homo sapiens |
| 5-formyluracil-DNA glycosylase | - |
Rattus norvegicus |
| SMUG1 | - |
Homo sapiens |
| SMUG1 | - |
Rattus norvegicus |
| uracil-DNA glycosylase | - |
Homo sapiens |
| uracil-DNA glycosylase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
| 7.5 | - |
assay at | Rattus norvegicus |
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