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Literature summary for 3.2.2.27 extracted from
- Characterization of the full length uracil-DNA glycosylase in the extreme thermophile Thermotoga maritima (2001), Mutat. Res., 485, 187-195.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tmudg, expression of His-tagged TMUDG in Escherichia coli strain BW310(DE3), TMUDG can substitute for the endogenous enzyme in Escherichia coli | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | weak product inhibition of TMUDG by uracil | Thermotoga maritima |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20578 | - |
x * 20578, sequence calculation | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
gene tmudg | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged TMUDG from Escherichia coli strain BW310(DE3) by nickel affinity and ion exchange chromatography, tag cleavage by thrombin | Thermotoga maritima |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimal growth temperature of the organism is 80-90°C | Thermotoga maritima | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme initiates base excision repair, BER, using a closed circular DNA substrate containing a unique U-G base pair | Thermotoga maritima | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20578, sequence calculation | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TMUDG | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
the enzyme has an optimal activity temperature below the growth temperature of the organism of 80-90°C | Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 85 | - |
Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat inactivation kinetics of the truncated and full length TMUDG, overview | Thermotoga maritima |
| 70 | 75 | highly stable | Thermotoga maritima |
| 75 | - |
purified recombinant enzyme, 2.5 h, completely stable | Thermotoga maritima |
| 85 | - |
purified recombinant enzyme, after 15 min completely stable, after 30 min loss of 80% of activity | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermotoga maritima |
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Release 2023.1 (January 2023)
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