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Literature summary for 3.2.2.27 extracted from
- Identification of a prototypical single-stranded uracil DNA glycosylase from Listeria innocua (2017), DNA Repair, 57, 107-115 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Listeria innocua |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S67M | inactive | Listeria innocua |
| S67M/S68N | the mutant shows reduced activity compared to the wild type enzyme | Listeria innocua |
| S68N | the mutant shows reduced activity compared to the wild type enzyme | Listeria innocua |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | complete inhibition at 5 mM | Listeria innocua |  |
| Co2+ | about 10% residual activity at 5 mM | Listeria innocua | |
| Cu2+ | complete inhibition at 5 mM | Listeria innocua | |
| Fe2+ | complete inhibition at 5 mM | Listeria innocua | |
| Ni2+ | complete inhibition at 5 mM | Listeria innocua | |
| Zn2+ | complete inhibition at 5 mM | Listeria innocua | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | highest activity at 5 mM Mg2+ | Listeria innocua | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-containing single-stranded DNA + H2O | Listeria innocua | - |
uracil + single-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Listeria innocua | Q926U4 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no enzymatic activity is detected with double-stranded and single-stranded hypoxanthine-, xanthine-, 5-hydroxymethyluracil-, 5-hydroxyuracil-, 8-oxoguanine-, 8-oxoadenine-, 5,6-dihydroxyuracil, 5-hydroxycytosine-, thymine glycol-, N6-methyladenine-, O6-methylguanine-, and ethenoadenine-containing DNA | Listeria innocua | ? | - |
? | |
| uracil-containing single-stranded DNA + H2O | - |
Listeria innocua | uracil + single-stranded DNA with abasic site | - |
? | |
| uracil-containing single-stranded DNA + H2O | best substrate | Listeria innocua | uracil + single-stranded DNA with abasic site | - |
? | |
| uracil-mismatched 13 nt bubbled double-stranded DNA + H2O | low activity | Listeria innocua | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched 19 nt bubbled double-stranded DNA + H2O | second best substrate | Listeria innocua | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched 19 nt shift bubbled double-stranded DNA + H2O | low activity | Listeria innocua | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched 7 nt bubbled double-stranded DNA + H2O | low activity | Listeria innocua | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | low activity | Listeria innocua | uracil + double-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| single-strand selective uracil DNA glycosylase | - |
Listeria innocua |
| SMUG1-like glycosylase | - |
Listeria innocua |
| UDG | - |
Listeria innocua |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Listeria innocua |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Listeria innocua |
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Release 2023.1 (January 2023)
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