Information on EC 3.2.1.26 - beta-fructofuranosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.26
-
RECOMMENDED NAME
GeneOntology No.
beta-fructofuranosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
-
-
-
-
transglycosylation
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Galactose metabolism
-
-
Metabolic pathways
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-
metabolism of disaccharids
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-
Starch and sucrose metabolism
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-
sucrose degradation III (sucrose invertase)
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-
sucrose degradation V (sucrose alpha-glucosidase)
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-
SYSTEMATIC NAME
IUBMB Comments
beta-D-fructofuranoside fructohydrolase
Substrates include sucrose; also catalyses fructotransferase reactions.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-57-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain IFO 3062
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-
Manually annotated by BRENDA team
strain IFO 3062
-
-
Manually annotated by BRENDA team
strain K-1
-
-
Manually annotated by BRENDA team
strain K-1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain JN19
-
-
Manually annotated by BRENDA team
TIT-KJ1
-
-
Manually annotated by BRENDA team
Aspergillus niger IMI 303386
strain IMI 303386
-
-
Manually annotated by BRENDA team
strain TS
-
-
Manually annotated by BRENDA team
strain IPT-301
-
-
Manually annotated by BRENDA team
strain KB
-
-
Manually annotated by BRENDA team
IFO 4476
-
-
Manually annotated by BRENDA team
strain IPT-329
-
-
Manually annotated by BRENDA team
ATCC 20524
-
-
Manually annotated by BRENDA team
2 invertases: I and II
-
-
Manually annotated by BRENDA team
ATCC 4412
-
-
Manually annotated by BRENDA team
strain G1
UniProt
Manually annotated by BRENDA team
strain G1
UniProt
Manually annotated by BRENDA team
gene cscA, strain DSM 10140
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Corynebacterium murisepticum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
synonym Eremothecium gossypii
UniProt
Manually annotated by BRENDA team
synonym Eremothecium gossypii
UniProt
Manually annotated by BRENDA team
IAM 5009
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
CRL 1100
-
-
Manually annotated by BRENDA team
CRL 1100
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Thunb. cv. Nellie White
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Nicotiana tabacum L. c.v. BY-2
strain L. c.v. BY-2
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-
Manually annotated by BRENDA team
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
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-
Manually annotated by BRENDA team
isozymes alkaline and acid invertases, i.e. IT I and IT II
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-
Manually annotated by BRENDA team
var. sojae
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-
Manually annotated by BRENDA team
x grandidentata
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Batsch var. vulgaris Maxim. Hakuto
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain FH4C
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-
Manually annotated by BRENDA team
strain GCB-IV
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-
Manually annotated by BRENDA team
strain X 2180
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-
Manually annotated by BRENDA team
DSM 4359
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
precursor
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain ATCC MYA-131
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-kestose + H2O
?
show the reaction diagram
1-kestose + H2O
D-fructose + ?
show the reaction diagram
1-kestose + H2O
D-fructose + D-glucose + sucrose
show the reaction diagram
-
-
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O
D-glucose + D-fructose + ?
show the reaction diagram
sucrose, also used to measure transfructosylating activity at high substrate concentration, 0.2 M sodium acetate buffer, pH 5.6, 50°C
products are mainly glucose and fructose, but also neokestose, 1-kestose, and nystose are built
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose + H2O
D-glucose + ?
show the reaction diagram
1-kestose, about 25% activity compared to sucrose, 0.2 M sodium acetate buffer, pH 5.6, 50°C
-
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranosyl-(1-2)-beta-D-fructofuranose + H2O
D-glucose + ?
show the reaction diagram
nystose, about 4% activity compared to sucrose, 0.2 M sodium acetate buffer, pH 5.6, 50°C
-
-
?
alpha-D-glucopyranosyl-(1,6)-D-fructofuranose + H2O
alpha-D-glucopyranose + D-fructofuranose
show the reaction diagram
palatinose, about 2% activity compared to sucrose, 0.2 M sodium acetate buffer, pH 5.6, 50°C
-
-
?
alpha-D-glucopyranosyl-(1-2)-beta-D-fructofuranose
glucose
show the reaction diagram
-
sucrose in 50 mM sodium acetate buffer, pH 5.5, 30 degrees Celsius
-
-
?
beta-methyl fructoside + H2O
?
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
?
show the reaction diagram
-
-
-
-
?
chicory inulin + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
?
fructooligosaccharide + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
fructosylnystose + H2O
D-fructose + ?
show the reaction diagram
-
-
-
?
grass juice + H2O
?
show the reaction diagram
-
hydrolysis of polyfructose moieties in agriculturally-sourced grass juice with recombinant truncated cytosolic enzyme results in the release of more than 13 mg/ml more bioavailable fructose than is measured in untreated grass juice. Bioethanol yields from fermentation experiments with Brewer's yeast and grass juice plus enzymeaere more than 25% higher than those achieved using untreated grass juice feedstock
-
-
?
inulin
D-fructose
show the reaction diagram
-
6% of the activity with fructooligosaccharides
-
-
?
inulin + H2O
?
show the reaction diagram
inulin + H2O
beta-D-fructose
show the reaction diagram
-
1.8% of the activity with sucrose, isoenzyme AIV I and AIV II
-
-
?
inulin + H2O
D-fructose
show the reaction diagram
inulin + H2O
D-fructose + ?
show the reaction diagram
inulin + H2O
D-fructose + D-glucose
show the reaction diagram
-
product ratio of 10.3:1
-
?
inulin + H2O
fructose
show the reaction diagram
isoamyl alcohol + H2O
?
show the reaction diagram
-
-
-
-
?
levan + H2O
?
show the reaction diagram
levan + H2O
D-fructose
show the reaction diagram
melizitose + H2O
?
show the reaction diagram
methyl-beta-D-fructofuranoside + H2O
?
show the reaction diagram
-
-
-
-
?
neokestose + H2O
?
show the reaction diagram
nystose + H2O
?
show the reaction diagram
nystose + H2O
D-fructose + ?
show the reaction diagram
nystose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
nystose + H2O
D-fructose + D-glucose + sucrose
show the reaction diagram
-
-
-
?
raffinose
beta-D-fructose + melibiose
show the reaction diagram
raffinose + H2O
alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose
show the reaction diagram
raffinose + H2O
beta-D-fructose + alpha-D-melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
beta-D-fructose + melibiose
show the reaction diagram
raffinose + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
raffinose + H2O
D-fructose + D-glucose
show the reaction diagram
-
13.5% of the activity with sucrose
-
-
?
raffinose + H2O
D-fructose + melibiose
show the reaction diagram
raffinose + H2O
fructose + melibiose
show the reaction diagram
raffinose + H2O
sucrose + D-fructose
show the reaction diagram
-
-
-
-
ir
Raftiline HP + H2O
D-glucose + D-fructose
show the reaction diagram
low activity
-
-
?
raftiline LS + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
?
raftilose + H2O
D-fructose
show the reaction diagram
best substrate for CscA
-
-
?
stachyose + H2O
?
show the reaction diagram
sucrose
1-kestose
show the reaction diagram
-
fructosyltransferase activity at high sucrose concentrations
-
?
sucrose
beta-D-fructose + alpha-D-glucose
show the reaction diagram
sucrose
fructooligosaccharides + glucose
show the reaction diagram
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
sucrose + H2O
fructose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
?
sucrose + lactose
lactosucrose
show the reaction diagram
trehalose + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
inulin + H2O
D-fructose + D-glucose
show the reaction diagram
P00724
-
product ratio of 10.3:1
-
?
raffinose + H2O
sucrose + D-fructose
show the reaction diagram
-
-
-
-
ir
sucrose
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
soluble AI activity in mungbean hypocotyl is regulated by multiple distinct mechanisms. Rapid down-regulation of soluble acid invertase and the Vr-AI1 transcript level. The speed of down-regulation is markedly reduced in the presence of sucrose, indole-3-acetic acid and light, respectively
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
Sac A alone is unable to allow growth on sucrose. A mutant strain, ZM4S, not able to grow on sucrose lacks the two sucrases SacB and SacC, but SacA is present in the intracellular form
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
sucrose + H2O
fructose + D-glucose
show the reaction diagram
P93199, P93291
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
arsenate
-
or phosphate is required
Cu(NO3)2
-
0.001-1 mM stimulates
Fe2+
-
5 mM, 2.4fold stimulation
K4Fe(CN)6
phosphate
-
or arsenate is required
Sr2+
-
activates
Zn2+
-
126% activity at 1 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,5-anhydro-D-mannose
-
competitive
2,5-dideoxy-2,5-imino-D-glucitol
-
-
2,5-dideoxy-2,5-imino-D-mannitol
-
very good inhibitor
2-Amino-2-hydroxymethyl propane-1,3-diol
-
extracellular enzyme
2-mercaptoethanol
4-chloromercuribenzoate
-
0.5 mM, 62% inhibition of isozyme IT I, 86% inhibition of isozyme IT II
4-hydroxymercuribenzoate
complete inhibition at 1 mM
AgCl
-
47.08% residual activity at 0.5 mM
AgNO3
-
0.1 mM, 37% inhibition; 1 mM, 88% inhibition
Al2(SO4)3
-
18.35% residual activity at 0.5 mM
Al3+
-
20.5% residual activity at 10 mM
alpha-chymotrypsin
-
0.5 mg/ml
-
alpha-methyl glucoside
-
50 mM, 20% inhibition
ammonium heptamolybdate
-
-
ammonium sulfate
-
intracellular enzyme
anabasine
-
-
aniline
Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 1
-
cell-wall invertase: 69% activity of control
-
Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 2
-
cell-wall invertase: 67% activity of control
-
ascorbic acid
-
-
ATP
-
inhibition of neutral invertase, no inhibition of alkaline invertase
Beta vulgaris cell wall/vacuolar inhibitor of fructosidase
-
cell-wall invertase: 57% activity of control
-
beta-methyl-fructoside
-
336 mM, 27% inhibition
CaCl2
-
72.27% residual activity at 0.5 mM
Cd-acetate
-
10 mM, 50% inhibition
cell-wall inhibitor of beta-fructosidase
-
-
-
cellobiose
-
40 mM, 22% inhibition
cellwall inhibitor of beta-fructosidase
2.6 A crystal structure of cell-wall invertase 1 in complex with protein inhibitor, cell-wall inhibitor of beta-fructosidase, from tobacco. The structure recognizes a small amino acid motif in the inhibitor that directly targets the invertase active site. The activity of INV1 and its interaction with inhibitor are strictly pH-dependent with a maximum at about pH 4.5. At this pH, isothermal titration calorimetry reveals that the inhibitor tightly binds its target with nanomolar affinity
-
CTP
-
inhibition of neutral invertase, no inhibition of alkaline invertase
D-fructose
D-glucose
dithiothreitol
-
-
endogenous protein inhibitors
fructose
fructose 1,6-diphosphate
-
-
fructose 6-phosphate
-
slight inhibition
fructoside
-
-
galactose
-
50 mM, 10% inhibition
glucose
GTP
-
inhibition of neutral invertase, no inhibition of alkaline invertase
guanidinium hydrochloride
-
2 M, 2% residual activity
Heptamolybdate
-
-
Inositol
-
-
invertase inhibitor of Solanum lycopersicum
-
6.7 nM tomato invertase with 1-2.5 nM inhibitor retains more than 90% invertase activity, 3-4 nM less than 90% activity, 5 nM about 75%, about 6.7 nM about 60% activity, pH 4.9, 37 degrees Celsius, 100 mM sucrose as substrate, proteinaceous functional inhibitor is expressed in leaves, flowers, and green fruit, proteolytically cleaving the invertase
-
INVINH1
iodoacetamide
-
5 mM, about 20% inhibition of isozyme IT I, 44% inhibition of isozyme IT II
K2PtCl3
-
non-competitive
KCl
-
70% residual activity at 0.5 mM
lactose
Lectins
-
lectins which bind mannose and glucose, and those which bind oligomers of N-acetylglucosamine
-
Maleic acid
-
-
malic acid
-
-
maltose
-
80 mM, 40% inhibition
Man-alpha(1-6)-[Xyl-beta(1-2)]-Man-beta(1-4)-GlcNAc-beta(1-4)-[Fuc-alpha(1-3)]-GlcNAc
-
pineapple stem bromelain glycopeptide MUXF, complete inhibition
melezitose