Information on EC 3.2.1.26 - beta-fructofuranosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.2.1.26
-
RECOMMENDED NAME
GeneOntology No.
beta-fructofuranosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
show the reaction diagram
-
-
-
-
hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
show the reaction diagram
double-displacement reaction mechanism, cleavage of beta-fructopyranosidic linkages involving a nucleophilic aspartate and a catalytic glutamic acid acting as general acid/base, structure of the active site substrate binding pocket possessing three binding subsites
O33833
hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
show the reaction diagram
the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism
-
hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
show the reaction diagram
the enzyme reaction mechanism with retention of the anomeric configuration at the site of cleavage, active site structure
O33833
hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
show the reaction diagram
the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism
Arthrobacter globiformis IFO 3062
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis
-
-
hydrolysis
-
-
hydrolysis
-
-
hydrolysis
-
-
hydrolysis
O81083
-
hydrolysis
-
-
hydrolysis
-
-
hydrolysis
-
-
hydrolysis
-
-
hydrolysis
-
-
hydrolysis of O-glycosyl bond
-
-
-
-
transglycosylation
O81083
-
PATHWAY
KEGG Link
MetaCyc Link
Galactose metabolism
-
Metabolic pathways
-
Starch and sucrose metabolism
-
sucrose degradation III (sucrose invertase)
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-fructofuranoside fructohydrolase
Substrates include sucrose; also catalyses fructotransferase reactions.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acid invertase
-
-
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
acid invertase
-
-
Acid sucrose-6-phosphate hydrolase
-
-
-
-
acINV
O81083
Allium cepa invertase
alkaline invertase
-
-
-
-
alkaline invertase
-
-
Atbetafruct4
-
-
beta-(1-2)-fructofuranosidase
Q564C7
-
beta-D-fructofuranosidase
-
has both fructosyltransferase and hydrolytic activities
beta-D-fructofuranosidase
-
-
beta-D-fructofuranosidase
-
has both fructosyltransferase and hydrolytic activities
beta-D-fructofuranosidase
Aspergillus oryzae IPT-301
-
has both fructosyltransferase and hydrolytic activities
-
beta-D-fructofuranosidase
-
has both fructosyltransferase and hydrolytic activities
beta-D-fructofuranosidase
Aureobasidium pullulans IPT-329
-
has both fructosyltransferase and hydrolytic activities
-
beta-D-fructofuranosidase
-
-
beta-D-fructofuranosidase
-
-
beta-D-fructofuranosidase fructohydrolase
-
-
beta-D-fructofuranosidase fructohydrolase
Saccharomyces cerevisiae GCB-IV
-
-
-
beta-D-fructofuranoside fructohydrolase
Q2PCS4
-
beta-D-fructofuranoside fructohydrolase
-
-
beta-FFase
-
-
-
-
beta-FFase
Arthrobacter globiformis IFO 3062
-
-
-
beta-fructofuranosidase
-
-
-
-
beta-fructofuranosidase
-
-
beta-fructofuranosidase
-
shows also transfructosylating activity
beta-fructofuranosidase
B8YJM2
-
beta-fructofuranosidase I
-
-
beta-fructosidase
-
-
-
-
beta-fructosidase
-
-
beta-fructosidase
-
-
beta-fructosidase
-
-
beta-fructosidase
O33833
-
beta-fructosidase
-
-
beta-h-fructosidase
-
-
-
-
cell wall invertase 4
Q8W413
-
cell wall-bound invertase
-
-
cell wall-bound ivertase
-
-
cell-wall invertase
-
CWI
cell-wall invertase
-
-
cell-wall invertase
-
-
cell-wall invertase
A2X5P7, Q01IS7, Q01IS8
-
cell-wall invertase
-
three genes PaxgINV1, 2, 3 with higher intraspecific homology than with other dicotyledons
cell-wall invertase 1
-
-
Cin1
Chenopodium rubrum
-
-
Cin1
Chenopodium rubrum
Q42691
-
Cin5
-
-
cwINV1
Q43866
-
Ffase
Aspergillus japonicus JN19
-
-
-
Ffh
Saccharomyces cerevisiae GCB-IV
-
-
-
fructofuranosidase, beta-
-
-
-
-
fructosylinvertase
-
-
-
-
glucosucrase
-
-
-
-
INAC-INV
-
-
INCW2
-
-
Inv-V
-
-
INV1p
Q2PCS4
-
invertase
-
-
-
-
invertase
-
-
invertase
Q0ZR36
-
invertase
Q2XQ19, Q2XQ20, Q2XQ21
-
invertase
Chenopodium rubrum
-
-
invertase
-
-
invertase
-
-
invertase
-
-
invertase
-
-
invertase
-
-
invertase
-
-
invertase
-
-
invertase
-
-
invertase
-
-
invertase
O33833
-
invertase
Q2PCS4
-
invertase
-
-
invertase
-
-
invertase
-
INVA and chimeric fusion enzyme INVA-CBD
Invertase E1
-
-
-
-
invertin
-
-
-
-
lbbetafruct2
Q94C06
-
lbbetafruct3
Q94C05
-
Lyc e 2.01
-
-
Lyc e 2.02
-
-
neutral invertase
-
-
neutral invertase
-
-
neutral invertase
-
-
OsCIN1
A2X5P7
-
OsCIN2
Q01IS7
-
OsCIN3
Q01IS8
-
Protein B46
-
-
-
-
re-INVB
-
free and immobilized enzyme
saccharase
-
-
-
-
saccharase
-
-
SAI
-
soluble acid invertase
soluble acid invertase
Q94C05, Q94C06
-
soluble acid invertase
-
-
SucB
Q0ZR36
shows also transfructosylation activity of 20 to 30% of total activity at sucrose concentrations up to 400 mM
sucrase
-
-
-
-
Sucrase E1
-
-
-
-
Sucrose-6-phosphate hydrolase
-
-
-
-
TIV-1
P29000
-
Uninv
C0LIF8
-
Vacuolar invertase
-
-
-
-
Vacuolar invertase
O81083
-
Vacuolar invertase
-
Bobetafruct3
Vacuolar invertase
-
VI
Vacuolar invertase
Q94C05, Q94C06
-
Vacuolar invertase
P29000
-
Vacuolar invertase
-
-
vacuolar invertase 1
-
-
VIN1
C4PBL7
-
yeast invertase
B8YJM2
-
maxinvert L 1000
-
-
-
-
additional information
-
the enzyme belongs to glycoside hydrolase family GH-32
additional information
O33833
the enzyme belongs to the glycoside hydrolase family GH32
CAS REGISTRY NUMBER
COMMENTARY
9001-57-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
cv. Tenshin
-
-
Manually annotated by BRENDA team
strain IFO 3062
-
-
Manually annotated by BRENDA team
Arthrobacter globiformis IFO 3062
strain IFO 3062
-
-
Manually annotated by BRENDA team
strain K-1
-
-
Manually annotated by BRENDA team
strain K-1
-
-
Manually annotated by BRENDA team
strain JN19
-
-
Manually annotated by BRENDA team
Aspergillus japonicus JN19
strain JN19
-
-
Manually annotated by BRENDA team
Aspergillus japonicus TIT-KJ1
TIT-KJ1
-
-
Manually annotated by BRENDA team
ATCC 20611
-
-
Manually annotated by BRENDA team
putative intracellular invertase
UniProt
Manually annotated by BRENDA team
strain IMI 303386
-
-
Manually annotated by BRENDA team
strains ATCC 20611 and IPT-615
-
-
Manually annotated by BRENDA team
wild-type strain NIAB 280, and its derivative the catabolite-derepressed mutant strain M125
-
-
Manually annotated by BRENDA team
Aspergillus niger IMI 303386
strain IMI 303386
-
-
Manually annotated by BRENDA team
Aspergillus ochraceus TS
strain TS
-
-
Manually annotated by BRENDA team
strain IPT-301
-
-
Manually annotated by BRENDA team
strain KB
-
-
Manually annotated by BRENDA team
Aspergillus oryzae IPT-301
strain IPT-301
-
-
Manually annotated by BRENDA team
Aspergillus oryzae KB
strain KB
-
-
Manually annotated by BRENDA team
IFO 4476
-
-
Manually annotated by BRENDA team
strain DSM 2404
-
-
Manually annotated by BRENDA team
strain IPT-329
-
-
Manually annotated by BRENDA team
Aureobasidium pullulans IPT-329
strain IPT-329
-
-
Manually annotated by BRENDA team
ATCC 20524
-
-
Manually annotated by BRENDA team
2 invertases: I and II
-
-
Manually annotated by BRENDA team
isozyme betafruct1; three isozymes betafruct1-3
SwissProt
Manually annotated by BRENDA team
isozyme betafruct2; three isozymes betafruct1-3
SwissProt
Manually annotated by BRENDA team
isozyme betafruct3; three isozymes betafruct1-3
SwissProt
Manually annotated by BRENDA team
alkaline invertase and various typs of acid invertase
-
-
Manually annotated by BRENDA team
Bifidobacterium adolescentis G1
strain G1
UniProt
Manually annotated by BRENDA team
gene cscA, strain DSM 10140
SwissProt
Manually annotated by BRENDA team
Chenopodium rubrum
-
-
-
Manually annotated by BRENDA team
Chenopodium rubrum
CIN1; red goosefoot, or pig weed
SwissProt
Manually annotated by BRENDA team
Corynebacterium murisepticum
-
-
-
Manually annotated by BRENDA team
IAM 5009
-
-
Manually annotated by BRENDA team
Fusarium oxysporum IAM 5009
IAM 5009
-
-
Manually annotated by BRENDA team
Jerusalem artichoke
-
-
Manually annotated by BRENDA team
isozyme lbbetafruct2; cv. Tainong 57
SwissProt
Manually annotated by BRENDA team
isozyme lbbetafruct3; cv. Tainong 57
SwissProt
Manually annotated by BRENDA team
L. cv. Tainong 57
Uniprot
Manually annotated by BRENDA team
Lactobacillus reuteri CRL 1100
CRL 1100
-
-
Manually annotated by BRENDA team
Thunb. cv. Nellie White
-
-
Manually annotated by BRENDA team
isoform INV1
UniProt
Manually annotated by BRENDA team
isoform INV2
-
-
Manually annotated by BRENDA team
cultivar Starkrimson
-
-
Manually annotated by BRENDA team
two isozymes from cell wall and soluble fraction
-
-
Manually annotated by BRENDA team
strain L. c.v. BY-2
-
-
Manually annotated by BRENDA team
Nicotiana tabacum L. c.v. BY-2
strain L. c.v. BY-2
-
-
Manually annotated by BRENDA team
japonica cv. Nipponbsare wild-type, F2 mutant from a cross of Oscyt-inv1 mutant with Oryza saliva induce cv. Kasalath wild-type
-
-
Manually annotated by BRENDA team
OsCIN1; Minghui 86
UniProt
Manually annotated by BRENDA team
OsCIN2; Minghui 86
UniProt
Manually annotated by BRENDA team
OsCIN3; Minghui 86
UniProt
Manually annotated by BRENDA team
several cell-wall invertase isozymes encoded by genes CIN1-9, the vacuolar isozymes are encoded by genes VIN
-
-
Manually annotated by BRENDA team
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
-
-
Manually annotated by BRENDA team
isozymes alkaline and acid invertases, i.e. IT I and IT II
-
-
Manually annotated by BRENDA team
x grandidentata
-
-
Manually annotated by BRENDA team
Batsch var. vulgaris Maxim. Hakuto
-
-
Manually annotated by BRENDA team
extracellular invertase and intracellular invertase
-
-
Manually annotated by BRENDA team
strain GCB-IV
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae FH4C
strain FH4C
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae GCB-IV
strain GCB-IV
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae X
strain X 2180
-
-
Manually annotated by BRENDA team
acid invertase and neutral invertase
-
-
Manually annotated by BRENDA team
salt sensitive cultivar CP 77-400
-
-
Manually annotated by BRENDA team
wild-type strains and several mutant ird strains, overview
-
-
Manually annotated by BRENDA team
isoform LIN6
Uniprot
Manually annotated by BRENDA team
var. Moneymaker wild-type and with silenced leaf isoforms of cw-Inv, interaction with Xanthomonas campestris pv vesicatoria infection
-
-
Manually annotated by BRENDA team
strain MSB8, DSM 3109
SwissProt
Manually annotated by BRENDA team
uncultured bacterium
UniProt
Manually annotated by BRENDA team
precursor
UniProt
Manually annotated by BRENDA team
fragment; (L.) Wilczek
UniProt
Manually annotated by BRENDA team
Chardonnay, Champagne
-
-
Manually annotated by BRENDA team
strain ATCC MYA-131
UniProt
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
F-2 genotype, gene Ivr2 encoding the vacuolar isozyme
Uniprot
Manually annotated by BRENDA team
gene Irv2
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
the isoforms EINV1, EINV2, EINV3 and EINV4 have the same molecular mass and catalytic properties such as Km for sucrose, pH optimum and temperature optimum, but they exhibit significant difference in pI values, thermal stability and chemical reactivity. The observed differences between isoforms arise from posttranslational modifications
physiological function
-
INV-E is associated with the development of the photosynthetic apparatus and the assimilation of nitrogen in Arabidopsis seedlings to control the ratio of sucrose content to hexose content
physiological function
O82119
role of LIN6 invertase during growth processes. In addition, LIN6 is regulated by a diurnal rhythm that drives LIN6 expression in subjective dawn
physiological function
Q8W413
T-DNA insertion lines of cell wall invertase 4 do not produce nectar. Overall nectary morphology appears to be normal, but mutant flowers accumulate higher than normal levels of starch in the receptacle, but not within the nectaries themselves. Conversely, wild-type, but not mutant, nectarial stomata stain intensely for starch. Cell wall extracts prepared from mutant flowers display greatly reduced invertase activity when compared with wild-type plants, and mutant flowers also accumulate significantly lower levels of total soluble sugar
physiological function
-
ectopic overexpression of invertase inhibitor INVINH1 in Arabidopsis thaliana specifically reduces cell wall invertase activity. By contrast, silencing its expression in tomato significantly increases the activity of cell wall invertase without altering activities of cytoplasmic and vacuolar invertases. Elevation of cell wall invertase activity in RNA interference transgenic tomato leads to a prolonged leaf life span involving in a blockage of abscisic acid-induced senescence and an increase in seed weight and fruit hexose level
physiological function
-
loss-of-function mutation in cell wall invertase INCW2 gene Mn1 results in the miniature1 seed phenotype. In wild-type seed, walls in growth develop uniformly in the basal endosperm transfer cell layer during 7 to 17 d after pollination, and the secretory/endocytic organelles proliferate in the basal endosperm transfer cells. Mitochondria accumulate in the vicinity of walls in growth. In the mutant basal endosperm transfer cells, walls in growth are stunted and their endoplasmic reticulum is swollen, Golgi density in the mutant basal endosperm transfer cells is 51% of the wild-type Golgi density. However, the polarized distribution of mitochondria is not affected
physiological function
-
single T-DNA insertion mutants of cytosolic isoforms cinv1 and cinv2 appear identical to wild-type plants on soil under our growth conditions. The cinv1/cinv2 double mutant plants flower and set seed when grown on soil, but are much smaller in all respects than wild-type plants at maturity. Seedlings of cinv1/cinv2 double mutant have relatively normal shoot growth, but drastically reduced root growth on solid medium without sugar. Whereas primary root extension over 7 days of growth is 60% of the wild-type value in cinv1 mutant, and 120% of the wild-type value in cinv2, it is only 17% of the wild-type value in cinv1/cinv2 double mutants. Cells in the root expansion zone of cinv1/cinv2 mutants are enlarged and have a greater tendency to collapse during manipulation than those of wild-type and single-mutant plants. Abnormal cell divisions occur in the stele, endodermis, and cortex. Growth on glucose restores root extension in double mutants to approximately half of wild-type. Neutral invertase activity in roots is 40% lower in double mutant than in wild-type seedlings
physiological function
-
invertases are important for gall development in clubroot disease of Brassicaceae caused by Plasmodiophora brassicae. Different transgenic lines expressing an invertase inhibitor under the control of two root-specific promoters, Pyk10 and CrypticT80, which results in the reduction of invertase activity, show clearly reduced clubroot symptoms in root tissue with highest promoter expression, whereas hypocotyl galls develop normally
physiological function
C4PBL7
transformation of cotton fiber with GhVIN1 RNA interference or overexpression constructs reduces or enhances fiber elongation
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-kestose + H2O
?
show the reaction diagram
-
-
-
-
?
1-kestose + H2O
?
show the reaction diagram
-
-
-
-
?
1-kestose + H2O
?
show the reaction diagram
-
-
-
-
?
1-kestose + H2O
?
show the reaction diagram
Aspergillus japonicus JN19
-
-
-
-
?
1-kestose + H2O
?
show the reaction diagram
Aspergillus japonicus TIT-KJ1
-
-
-
-
?
1-kestose + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
1-kestose + H2O
D-fructose + ?
show the reaction diagram
Q0ZR36
-
-
-
?
1-kestose + H2O
D-fructose + ?
show the reaction diagram
A1A2J8, -
-
-
-
?
1-kestose + H2O
D-fructose + ?
show the reaction diagram
C0LIF8
-
-
-
?
1-kestose + H2O
D-fructose + ?
show the reaction diagram
Bifidobacterium adolescentis G1
A1A2J8
-
-
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
-
sucrose
-
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
-
sucrose
-
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O
D-glucose + D-fructose
show the reaction diagram
-
sucrose, 0.2 M sodium acetate buffer, pH 4.9, 37°C
-
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O
D-glucose + D-fructose + ?
show the reaction diagram
B8YJM2, -
sucrose, also used to measure transfructosylating activity at high substrate concentration, 0.2 M sodium acetate buffer, pH 5.6, 50°C
products are mainly glucose and fructose, but also neokestose, 1-kestose, and nystose are built
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose + H2O
D-glucose + ?
show the reaction diagram
B8YJM2, -
1-kestose, about 25% activity compared to sucrose, 0.2 M sodium acetate buffer, pH 5.6, 50°C
-
-
?
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranosyl-(1-2)-beta-D-fructofuranose + H2O
D-glucose + ?
show the reaction diagram
B8YJM2, -
nystose, about 4% activity compared to sucrose, 0.2 M sodium acetate buffer, pH 5.6, 50°C
-
-
?
alpha-D-glucopyranosyl-(1,6)-D-fructofuranose + H2O
alpha-D-glucopyranose + D-fructofuranose
show the reaction diagram
B8YJM2, -
palatinose, about 2% activity compared to sucrose, 0.2 M sodium acetate buffer, pH 5.6, 50°C
-
-
?
alpha-D-glucopyranosyl-(1-2)-beta-D-fructofuranose
glucose
show the reaction diagram
-
sucrose in 50 mM sodium acetate buffer, pH 5.5, 30 degrees Celsius
-
-
?
beta-methyl fructoside + H2O
?
show the reaction diagram
-
-
-
-
?
fructooligosaccharide + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
fructosylnystose + H2O
D-fructose + ?
show the reaction diagram
A1A2J8, -
-
-
-
?
grass juice + H2O
?
show the reaction diagram
-
hydrolysis of polyfructose moieties in agriculturally-sourced grass juice with recombinant truncated cytosolic enzyme results in the release of more than 13 mg/ml more bioavailable fructose than is measured in untreated grass juice. Bioethanol yields from fermentation experiments with Brewer's yeast and grass juice plus enzymeaere more than 25% higher than those achieved using untreated grass juice feedstock
-
-
?
inulin
D-fructose
show the reaction diagram
-
6% of the activity with fructooligosaccharides
-
-
?
inulin + H2O
fructose
show the reaction diagram
-
-
-
-
?
inulin + H2O
fructose
show the reaction diagram
-
-
-
-
?
inulin + H2O
fructose
show the reaction diagram
-
-
-
?
inulin + H2O
fructose
show the reaction diagram
-
-
-
?
inulin + H2O
D-fructose
show the reaction diagram
O33833
-
-
-
?
inulin + H2O
beta-D-fructose
show the reaction diagram
-
1.8% of the activity with sucrose, isoenzyme AIV I and AIV II
-
-
?
inulin + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
inulin + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
inulin + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
inulin + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
inulin + H2O
D-fructose + ?
show the reaction diagram
A1A2J8, -
-
-
-
?
inulin + H2O
D-fructose + ?
show the reaction diagram
Bifidobacterium adolescentis G1
A1A2J8
-
-
-
?
isoamyl alcohol + H2O
?
show the reaction diagram
-
-
-
-
?
levan + H2O
?
show the reaction diagram
-
-
-
-
?
levan + H2O
?
show the reaction diagram
-
-
-
-
?
levan + H2O
D-fructose
show the reaction diagram
-
-
-
-
?
levan + H2O
D-fructose
show the reaction diagram
-, Q564C7
low activity
-
-
?
melizitose + H2O
?
show the reaction diagram
-
8.1% of the activity with sucrose
-
-
?
melizitose + H2O
?
show the reaction diagram
-
no activity with melizitose
-
-
-
melizitose + H2O
?
show the reaction diagram
-
no activity with melizitose
-
-
-
melizitose + H2O
?
show the reaction diagram
-
4.5% of the activity with sucrose
-
-
?
melizitose + H2O
?
show the reaction diagram
-
acid invertase, 4.6% of the activity with sucrose, alkaline invertase, 3.1% of the activity with sucrose
-
-
?
melizitose + H2O
?
show the reaction diagram
Nicotiana tabacum L. c.v. BY-2
-
8.1% of the activity with sucrose
-
-
?
neokestose + H2O
?
show the reaction diagram
A1A2J8, -
-
-
-
?
neokestose + H2O
?
show the reaction diagram
Bifidobacterium adolescentis G1
A1A2J8
-
-
-
?
nystose + H2O
?
show the reaction diagram
-
-
-
-
?
nystose + H2O
?
show the reaction diagram
-
-
-
-
?
nystose + H2O
?
show the reaction diagram
Aspergillus japonicus JN19
-
-
-
-
?
nystose + H2O
?
show the reaction diagram
Aspergillus japonicus TIT-KJ1
-
-
-
-
?
nystose + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
nystose + H2O
D-fructose + ?
show the reaction diagram
Q0ZR36
-
-
-
?
nystose + H2O
D-fructose + ?
show the reaction diagram
A1A2J8, -
-
-
-
?
nystose + H2O
D-fructose + ?
show the reaction diagram
C0LIF8
-
-
-
?
nystose + H2O
D-fructose + ?
show the reaction diagram
Bifidobacterium adolescentis G1
A1A2J8
-
-
-
?
nystose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
raffinose
beta-D-fructose + melibiose
show the reaction diagram
Aspergillus ochraceus, Aspergillus ochraceus TS
-
96% of the activity with sucrose
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
-
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
no activity
-
-
-
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
soluble enzyme
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
hydrolyzed at 15% of the rate of sucrose
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
5.0% of the activity with sucrose
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
10% of the activity with sucrose
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
77% of the activity with sucrose
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
invertase I, at 45% of the activity with sucrose. Invertase II and III, at 30% of the activity with sucrose
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
-
no activity with alkaline invertase, hydrolysis with neutral invertase
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
Lactobacillus reuteri CRL 1100
-
-
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
Nicotiana tabacum L. c.v. BY-2
-
77% of the activity with sucrose
-
-
?
raffinose + H2O
fructose + melibiose
show the reaction diagram
Aspergillus japonicus TIT-KJ1
-
-
-
-
?
raffinose + H2O
beta-D-fructose + alpha-D-melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
beta-D-fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
beta-D-fructose + melibiose
show the reaction diagram
-, Q9ZTX2
-
-
-
?
raffinose + H2O
beta-D-fructose + melibiose
show the reaction diagram
-
about 20% of the activity with sucrose, isoenzyme AIV I and AIV II
-
-
?
raffinose + H2O
beta-D-fructose + melibiose
show the reaction diagram
-
about 30% of the activity with sucrose, isoenzyme AIV I and AIV II
-
-
?
raffinose + H2O
beta-D-fructose + melibiose
show the reaction diagram
-
acid invertase, 38.9% of the activity with sucrose, alkaline invertase, 40.0% of the activity with sucrose
-
-
?
raffinose + H2O
sucrose + D-fructose
show the reaction diagram
-
-
-
-
ir
raffinose + H2O
alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose
show the reaction diagram
O33833
-
-
-
?
raffinose + H2O
alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose
show the reaction diagram
Q94C05, Q94C06
25% of the activity with sucrose, recombinant isozyme lbbetafruct3
-
-
?
raffinose + H2O
alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose
show the reaction diagram
Q94C05, Q94C06
37% of the activity with sucrose, recombinant isozyme lbbetafruct2
-
-
?
raffinose + H2O
alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose
show the reaction diagram
-
isozymes IT I and IT II
-
-
?
raffinose + H2O
D-fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
D-fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
D-fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
D-fructose + melibiose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
D-fructose + melibiose
show the reaction diagram
C0LIF8
-
-
-
?
raffinose + H2O
D-fructose + melibiose
show the reaction diagram
-
lower activity of all mutants than of wild-type
-
-
?
raffinose + H2O
D-fructose + melibiose
show the reaction diagram
Aspergillus japonicus JN19
-
-
-
-
?
raffinose + H2O
D-fructose + ?
show the reaction diagram
-
-
-
-
?
raffinose + H2O
D-fructose + D-glucose
show the reaction diagram
-
13.5% of the activity with sucrose
-
-
?
Raftiline HP + H2O
D-glucose + D-fructose
show the reaction diagram
-, Q564C7
low activity
-
-
?
raftiline LS + H2O
D-glucose + D-fructose
show the reaction diagram
-, Q564C7
-
-
-
?
raftilose + H2O
D-fructose
show the reaction diagram
-, Q564C7
best substrate for CscA
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
invertase I, at 45% of the activity with sucrose. Invertase II and III, at 30% of the activity with sucrose
-
-
?
stachyose + H2O
?
show the reaction diagram
-
no activity with alkaline invertase, hydrolysis with neutral invertase
-
-
?
stachyose + H2O
?
show the reaction diagram
-
2.6% of the activity with sucrose
-
-
?
stachyose + H2O
?
show the reaction diagram
-
about 20% of the activity with sucrose, isoenzyme AIV I and AIV II
-
-
?
stachyose + H2O
?
show the reaction diagram
Q94C05, Q94C06
13% of the activity with sucrose, recombinant isozyme lbbetafruct2
-
-
?
stachyose + H2O
?
show the reaction diagram
Q94C05, Q94C06
9% of the activity with sucrose, recombinant isozyme lbbetafruct3
-
-
?
stachyose + H2O
?
show the reaction diagram
Lactobacillus reuteri CRL 1100
-
-
-
-
?
sucrose
1-kestose
show the reaction diagram
-
fructosyltransferase activity at high sucrose concentrations
-
?
sucrose
fructooligosaccharides + glucose
show the reaction diagram
-
-
-
?
sucrose
fructooligosaccharides + glucose
show the reaction diagram
-
-
converts sucrose to 61.2% fructooligosaccharides with a 50% concentration of sucrose as substrate, fructosyltransferase activity
?
sucrose
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-, soluble AI activity in mungbean hypocotyl is regulated by multiple distinct mechanisms. Rapid down-regulation of soluble acid invertase and the Vr-AI1 transcript level. The speed of down-regulation is markedly reduced in the presence of sucrose, indole-3-acetic acid and light, respectively
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
Corynebacterium murisepticum
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
beta-D-fructofuranose
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
beta-D-fructofuranose
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
alkaline invertase is highly specific for sucrose, neutral invertase also hydrolyzes raffinose and stachyose
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
Lactobacillus reuteri CRL 1100
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
Nicotiana tabacum L. c.v. BY-2
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
Aspergillus japonicus TIT-KJ1
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
Fusarium oxysporum IAM 5009
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
Saccharomyces cerevisiae X
-
-
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
Saccharomyces cerevisiae FH4C
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q8L897
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
P49175
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q43799
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
P29000
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
ir
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Chenopodium rubrum
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
ir
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
O33833
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Chenopodium rubrum
Q42691
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q2XQ19, Q2XQ20, Q2XQ21
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q0ZR36
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
O33833
best substrate
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-, Q564C7
low activity
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
preferred substrate, high activity on sugar cane bagasse, soybean waste, and wheat bran, saccharification levels are 93.69, 57.67, and 55.24, respectively
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
isozymes IT I and IT II
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q94C05, Q94C06
preferred substrate, recombinant isozyme lbbetafruct2
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q94C05, Q94C06
preferred substrate, recombinant isozyme lbbetafruct3
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q2PCS4, -
sucrose is the only substrate
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
5 mM sucrose, 50 mM Na-acetate, pH 5.2, and 0.02% Na-azide, 30°C, 60 min
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Arthrobacter globiformis IFO 3062
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Aspergillus japonicus JN19
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Aspergillus oryzae IPT-301, Aureobasidium pullulans IPT-329
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Saccharomyces cerevisiae GCB-IV
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
-
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-, Q9ZTX2
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
A1A2J8, -
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
Sac A alone is unable to allow growth on sucrose. A mutant strain, ZM4S, not able to grow on sucrose lacks the two sucrases SacB and SacC, but SacA is present in the intracellular form
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
10% of the activity with fructooligosaccharides
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
isoenzyme AIV I and AIV II
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
isoenzyme AIV I and AIV II
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
Aspergillus oryzae KB
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
Bifidobacterium adolescentis G1
A1A2J8
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
Aspergillus ochraceus TS
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
involved in root cell development and reproductivity in rice, 1% sucrose, 50 mM potassium phosphate, pH 7, 37 degrees Celsius, 30 min
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
100 mM sucrose, 1 mg/ml mitochondria, room temperature (22-25°C), 300 mM mannitol + 10 mM morpholinepropanesulfonic acid buffer, pH 7.4, no enzyme activity with maltose or raffinose
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
100 mM sucrose, 10 min, 55 degrees Celsius, 50 mM sodium acetate buffer, pH 4.5
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
37 degrees Celsius, 0.1 M sodium acetate, pH 5, no hydrolysis of cellobiose, maltose, lactose, inulin
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
O81083
50 mM MES-buffer, pH 5.6, 20 min, 28°C
at sucrose concentrations higher than 100 mM distinct but small amount of 1-kestose is found as transglycosylation product
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-, activity measured in whole fly homogenates, substrate concentration of 50 mM in sodium phosphate buffer
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
C0LIF8
highest activity at 20 mM
-
-
?
sucrose + lactose
lactosucrose
show the reaction diagram
-
transfructosylation mechanism of an ordered bi-bi type in which sucrose is bound first to the enzyme and lactosucrose is released last
-
-
?
trehalose + H2O
?
show the reaction diagram
-
slow hydrolysis
-
-
?
trehalose + H2O
?
show the reaction diagram
Nicotiana tabacum, Nicotiana tabacum L. c.v. BY-2
-
8.0% of the activity with sucrose
-
-
?
methyl-beta-D-fructofuranoside + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
-
additional information
?
-
-
high regiospecificity to transfer the fructosyl moiety for the 1-OH group of terminal fructofuranosides
-
-
?
additional information
?
-
-
specific to the beta-1,2 linkage between glucose and fructose
-
-
?
additional information
?
-
-
no sucrose:sucrose fructosyltransferase activity
-
-
?
additional information
?
-
-
at high substrate concentrations, 1 M, transferase activity, transferring the beta-D-fructofuranosyl residue to primary alcohols such as methanol, ethanol and n-propanol, extracellular enzyme
-
-
?
additional information
?
-
-
constitutive enzyme
-
-
?
additional information
?
-
-
enzyme is induced in presence of sucrose and suppressed in presence of glucose or maltose
-
-
?
additional information
?
-
-
alkaline invertase activity appears when the root begins to develop and simultaneously to synthesize sucrose - activity of enzyme form I is high during sucrose accumulation and the enzyme rapidly decreases when sucrose reaches a constant level, while the level of enzyme II activity is approximately constant, acid invertase activity is very high in immature roots, but rapidly decreases before sucrose is stored and is hardly detectable in mature roots
-
-
?
additional information
?
-
Corynebacterium murisepticum
-
not repressed by presence of glucose in the medium
-
-
-
additional information
?
-
-
Lys e 2 is able to trigger histamine release from passively sensitized basophils of patients with IgE to carbohydrate determinants
-
-
-
additional information
?
-
-
no hydrolysis of lactose and maltose
-
-
-
additional information
?
-
-
no hydrolysis of lactose, maltose, trehalose, melizitose and melibiose
-
-
-
additional information
?
-
-
no hydrolysis of maltose, cellobiose and lactose
-
-
-
additional information
?
-
-
no transfructosylation reraction at 1 M sucrose
-
-
-
additional information
?
-
-
acid invertases play a key role in sugar metabolism
-
-
-
additional information
?
-
-
cell wall and vacuole invertases are important metabolic enzymes, but also key players during wound and pathogen defense reactions and in several developmental transitions, post-translational regulation mechanisms, overview
-
-
-
additional information
?
-
-
cell-wall invertase isozymes CIN1-9 play an important role in carbon allocation to developing organs, and respond to rapidly to water deficit in antheres and and peduncles and through a reduction in sink strength help to coordinate a delay in anthesis and heading, their expression is affected by water and temperature stress, overview
-
-
-
additional information
?
-
-
in vivo substrate specificity of the wild-type and the derepressed mutant strains, overview
-
-
-
additional information
?
-
Daucus carota, Chenopodium rubrum
-
post-translational regulation of isozyme CWI involving inhibitor CIF
-
-
-
additional information
?
-
Chenopodium rubrum
-
the cell wall-bound isozyme Cin1 shows cytokinin-like function in leaf senescence, overview, the isozymes have different functions, e.g. the vacuolar isozyme is responsible for control of sugar composition in fruits and storage organs, for osmoregulation and cell enlargement, and response to drought stress, hypoxia, wounding, and gravitropism, the extracellular isozyme is involved in sucrose partitioning, and response to wounding and pathogen infection, several regulation mechanisms, overview
-
-
-
additional information
?
-
P49175, -
the enzyme is an important in carbohydrate metabolism, it is regulated by abiotic stress, the phytohormone abscisic acid, glucose, and may as well depend on the circadian rhythm in leaves, overview
-
-
-
additional information
?
-
-
the isozymes have different functions, e.g. the vacuolar isozyme is responsible for control of sugar composition in fruits and storage organs, for osmoregulation and cell enlargement, and response to drought stress, hypoxia, wounding, and gravitropism, the extracellular isozyme is involved in sucrose partitioning, and response to wounding and pathogen infection, several regulation mechanisms, overview
-
-
-
additional information
?
-
-
the isozymes have different functions, e.g. the vacuolar isozyme is responsible for control of sugar composition in fruits and storage organs, for osmoregulation and cell enlargement, and response to drought stress, hypoxia, wounding, and gravitropism, the extracellular isozyme is involved in sucrose partitioning, and response to wounding and pathogen infection, several regulation mechanisms, overview, the cell wall-bound isozyme is involved in establishing a higher sink strength in young seeds at the site of assimilate unloading into the apoplast
-
-
-
additional information
?
-
-
the isozymes have different functions, e.g. the vacuolar isozyme is responsible for control of sugar composition in fruits and storage organs, for osmoregulation and cell enlargement, and response to drought stress, hypoxia, wounding, and gravitropism, the extracellular isozyme is involved in sucrose partitioning, and response to wounding and pathogen infection, several regulation mechanisms, overview, the cell wall-bound isozyme is involved in nucellar projection in the endospermal transfer cell layer
-
-
-
additional information
?
-
Q8L897
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
P49175
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
-
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
P29000
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
-
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
Q43799
the isozymes play important roles in plant development, enzyme regulation, overview, the cell wall-bound isozyme responses to pathogen attack and wounding
-
-
-
additional information
?
-
-
the isozymes play important roles in plant development, enzyme regulation, overview, the cell wall-bound isozyme responses to pathogen attack and wounding
-
-
-
additional information
?
-
Q94C05, Q94C06
activity with cellobiose, maltose, lactose, and inulin, by isozyme lbbetafruct2
-
-
-
additional information
?
-
Q94C05, Q94C06
activity with cellobiose, maltose, lactose, and inulin, by isozyme lbbetafruct3
-
-
-
additional information
?
-
-
both isozymes IT I and IT II show no activity with maltose
-
-
-
additional information
?
-
-, Q564C7
no activity with melezitose, raffinose is a poor substrate
-
-
-
additional information
?
-
-
substrate specificity of isozymes SAI and CWI, no activity with stachyose of isozymes SAI and CWI
-
-
-
additional information
?
-
-
the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism, overview
-
-
-
additional information
?
-
Q0ZR36
does not use inulin and levan as substrates
-
-
-
additional information
?
-
-
no activity with maltose, lactose, turanose, leucrose, and palatinose
-
-
-
additional information
?
-
A2X5P7, Q01IS7, Q01IS8
function in seed development
-
-
-
additional information
?
-
-
function of cell wall-bound invertase activity during pathogen infection, the Lin8-mutant lines 33 and 57 do not increase activity in response to Xanthomonas campestris pv vesicatoria infection, line 50 only slightly, and all mutant lines show symptoms delayed, expression of senescent-associated genes is decreased, mutants have about 50% lower starch content due to 2-fold increased sucrose export, and show a tendency for increased fructose content
-
-
-
additional information
?
-
-
no substrate: trehalose
-
-
-
additional information
?
-
C0LIF8
enzyme does not show transfructosylating activity. No substrate: inulin
-
-
-
additional information
?
-
-
no substrate: stachyose, melizitose
-
-
-
additional information
?
-
Arthrobacter globiformis IFO 3062
-
the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism, overview
-
-
-
additional information
?
-
Aspergillus japonicus TIT-KJ1
-
specific to the beta-1,2 linkage between glucose and fructose
-
-
?
additional information
?
-
Aspergillus ochraceus TS
-
no hydrolysis of maltose, cellobiose and lactose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
raffinose + H2O
sucrose + D-fructose
show the reaction diagram
-
-
-
-
ir
sucrose
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
soluble AI activity in mungbean hypocotyl is regulated by multiple distinct mechanisms. Rapid down-regulation of soluble acid invertase and the Vr-AI1 transcript level. The speed of down-regulation is markedly reduced in the presence of sucrose, indole-3-acetic acid and light, respectively
-
-
?
sucrose + H2O
fructose + glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q8L897
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
P49175
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Q43799
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
P29000
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
ir
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Carica papaya, Chenopodium rubrum
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Chenopodium rubrum
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
ir
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
O33833
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Chenopodium rubrum
Q42691
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
high activity on sugar cane bagasse, soybean waste, and wheat bran, saccharification levels are 93.69, 57.67, and 55.24, respectively
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
-
Sac A alone is unable to allow growth on sucrose. A mutant strain, ZM4S, not able to grow on sucrose lacks the two sucrases SacB and SacC, but SacA is present in the intracellular form
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
involved in root cell development and reproductivity in rice
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
constitutive enzyme
-
-
?
additional information
?
-
-
enzyme is induced in presence of sucrose and suppressed in presence of glucose or maltose
-
-
?
additional information
?
-
-
alkaline invertase activity appears when the root begins to develop and simultaneously to synthesize sucrose - activity of enzyme form I is high during sucrose accumulation and the enzyme rapidly decreases when sucrose reaches a constant level, while the level of enzyme II activity is approximately constant, acid invertase activity is very high in immature roots, but rapidly decreases before sucrose is stored and is hardly detectable in mature roots
-
-
?
additional information
?
-
Corynebacterium murisepticum
-
not repressed by presence of glucose in the medium
-
-
-
additional information
?
-
-
Lys e 2 is able to trigger histamine release from passively sensitized basophils of patients with IgE to carbohydrate determinants
-
-
-
additional information
?
-
-
acid invertases play a key role in sugar metabolism
-
-
-
additional information
?
-
-
cell wall and vacuole invertases are important metabolic enzymes, but also key players during wound and pathogen defense reactions and in several developmental transitions, post-translational regulation mechanisms, overview
-
-
-
additional information
?
-
-
cell-wall invertase isozymes CIN1-9 play an important role in carbon allocation to developing organs, and respond to rapidly to water deficit in antheres and and peduncles and through a reduction in sink strength help to coordinate a delay in anthesis and heading, their expression is affected by water and temperature stress, overview
-
-
-
additional information
?
-
-
in vivo substrate specificity of the wild-type and the derepressed mutant strains, overview
-
-
-
additional information
?
-
Daucus carota, Chenopodium rubrum
-
post-translational regulation of isozyme CWI involving inhibitor CIF
-
-
-
additional information
?
-
Chenopodium rubrum
-
the cell wall-bound isozyme Cin1 shows cytokinin-like function in leaf senescence, overview, the isozymes have different functions, e.g. the vacuolar isozyme is responsible for control of sugar composition in fruits and storage organs, for osmoregulation and cell enlargement, and response to drought stress, hypoxia, wounding, and gravitropism, the extracellular isozyme is involved in sucrose partitioning, and response to wounding and pathogen infection, several regulation mechanisms, overview
-
-
-
additional information
?
-
P49175, -
the enzyme is an important in carbohydrate metabolism, it is regulated by abiotic stress, the phytohormone abscisic acid, glucose, and may as well depend on the circadian rhythm in leaves, overview
-
-
-
additional information
?
-
-
the isozymes have different functions, e.g. the vacuolar isozyme is responsible for control of sugar composition in fruits and storage organs, for osmoregulation and cell enlargement, and response to drought stress, hypoxia, wounding, and gravitropism, the extracellular isozyme is involved in sucrose partitioning, and response to wounding and pathogen infection, several regulation mechanisms, overview
-
-
-
additional information
?
-
-
the isozymes have different functions, e.g. the vacuolar isozyme is responsible for control of sugar composition in fruits and storage organs, for osmoregulation and cell enlargement, and response to drought stress, hypoxia, wounding, and gravitropism, the extracellular isozyme is involved in sucrose partitioning, and response to wounding and pathogen infection, several regulation mechanisms, overview, the cell wall-bound isozyme is involved in establishing a higher sink strength in young seeds at the site of assimilate unloading into the apoplast
-
-
-
additional information
?
-
-
the isozymes have different functions, e.g. the vacuolar isozyme is responsible for control of sugar composition in fruits and storage organs, for osmoregulation and cell enlargement, and response to drought stress, hypoxia, wounding, and gravitropism, the extracellular isozyme is involved in sucrose partitioning, and response to wounding and pathogen infection, several regulation mechanisms, overview, the cell wall-bound isozyme is involved in nucellar projection in the endospermal transfer cell layer
-
-
-
additional information
?
-
Q8L897
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
P49175
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
-
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
P29000
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
-
the isozymes play important roles in plant development, enzyme regulation, overview
-
-
-
additional information
?
-
Q43799
the isozymes play important roles in plant development, enzyme regulation, overview, the cell wall-bound isozyme responses to pathogen attack and wounding
-
-
-
additional information
?
-
-
the isozymes play important roles in plant development, enzyme regulation, overview, the cell wall-bound isozyme responses to pathogen attack and wounding
-
-
-
additional information
?
-
A2X5P7, Q01IS7, Q01IS8
function in seed development
-
-
-
additional information
?
-
-
function of cell wall-bound invertase activity during pathogen infection, the Lin8-mutant lines 33 and 57 do not increase activity in response to Xanthomonas campestris pv vesicatoria infection, line 50 only slightly, and all mutant lines show symptoms delayed, expression of senescent-associated genes is decreased, mutants have about 50% lower starch content due to 2-fold increased sucrose export, and show a tendency for increased fructose content
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
arsenate
-
or phosphate is required
Ba2+
-
activates
Ba2+
-
20% stimulation of activity at 1 mM
Ba2+
-
50% increased activity at 1 mM
Ba2+
-
1 mM enhances the extracellular activity about 154%, and enhances the intracellular activity about 202%
Ca2+
-
5 mM, 3.4fold stimulation
Co2+
-
activates
Co2+
-
5 mM, 1.7fold stimulation
Co2+
-
69% increased activity at 1 mM
Cu(NO3)2
-
0.001-1 mM stimulates
Cu2+
-
5 mM, 2fold stimulation
Cu2+
-
120% increased activity at 1 mM
Fe2+
-
5 mM, 2.4fold stimulation
K+
-
20% increased activity at 1 mM
K4Fe(CN)6
-
0.1 M, activates
K4Fe(CN)6
-
activates enzyme embedded within a polyacrylamide gel, no effect on CM-cellulose-bound enzyme
Mg2+
-
activates
Mg2+
-
1 mM, activation of invertase activity to 115% of control
Mg2+
-
5 mM, 3.5fold stimulation
Mg2+
-
50% stimulation of activity at 1 mM
Mg2+
-
105% increased activity at 1 mM
Mg2+
-
1 mM enhances the extracellular activity about 180%
Mn2+
-
activates
Mn2+
-
57% stimulation of activity at 1 mM
Na+
-
5 mM, 2fold stimulation
Na+
-
35% stimulation of activity at 1 mM
Na+
-
60% increased activity at 1 mM
phosphate
-
or arsenate is required
Sr2+
-
activates
Mn2+
-
1 mM enhances the intracellular activity about 317%
additional information
-, Q564C7
the enzyme is not affected by 1 mM Mn2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,5-anhydro-D-mannose
-
competitive
2,5-dideoxy-2,5-imino-D-glucitol
-
-
2,5-dideoxy-2,5-imino-D-mannitol
-
very good inhibitor
2-Amino-2-hydroxymethyl propane-1,3-diol
-
extracellular enzyme
2-mercaptoethanol
-
-
4-chloromercuribenzoate
-
0.5 mM, 62% inhibition of isozyme IT I, 86% inhibition of isozyme IT II
4-hydroxymercuribenzoate
-, Q564C7
complete inhibition at 1 mM
Ag+
-
0.004 mM, complete inhibition; AgNO3
Ag+
-
0.1 mM, 95% inhibition of the CM-cellulose adsorbed enzyme, enzyme embedded within a polyacrylamide gel is inhibited 72.7%
Ag+
-
62% inhibition at 0.01 mM, 89% inhibition at 1 mM
Ag+
-
0.02 mM, complete inhibition of invertase I, 44% inhibition of invertase II
Ag+
-
1.7 mM AgNO3, complete inhibition
Ag+
-
1 mM, 26% inhibition of beta-fructofuranosidase activity, no effect on invertase activity
Ag+
-
1 mM, 56% inhibition of isozyme IT I, 81% inhibition of isozyme IT II
Ag+
A1A2J8, -
10 mM, no residual activity
AgCl
-
47.08% residual activity at 0.5 mM
AgNO3
-
0.1 mM, 37% inhibition; 1 mM, 88% inhibition
Al2(SO4)3
-
18.35% residual activity at 0.5 mM
alpha-chymotrypsin
-
0.5 mg/ml
-
alpha-methyl glucoside
-
50 mM, 20% inhibition
ammonium heptamolybdate
-
-
ammonium sulfate
-
intracellular enzyme
Aniline
-
extracellular enzyme
Aniline
-
10 mM, 45% inhibition of invertase I, no inhibition of invertase II
Aniline
-
5 mM, 36% inhibition of isozyme IT I, 17% inhibition of isozyme IT II
Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 1
-
cell-wall invertase: 69% activity of control
-
Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 2
-
cell-wall invertase: 67% activity of control
-
ascorbic acid
-
-
ATP
-
inhibition of neutral invertase, no inhibition of alkaline invertase
Beta vulgaris cell wall/vacuolar inhibitor of fructosidase
-
cell-wall invertase: 57% activity of control
-
beta-methyl-fructoside
-
336 mM, 27% inhibition
Ca2+
-
CaCl2; inhibition of alkaline invertase no inhibition of neutral invertase
Ca2+
-
slight inhibition
Ca2+
-
5 mM, 35% inhibition of isozyme IT I, 84% inhibition of isozyme IT II
Ca2+
-, Q564C7
15% inhibition at 1 mM
Ca2+
-
73% residual activity at 1 mM
CaCl2
-
72.27% residual activity at 0.5 mM
Cd-acetate
-
10 mM, 50% inhibition
Cd2+
-
1 mM, 66% inhibition
Cd2+
-
strong inhibition
cell-wall inhibitor of beta-fructosidase
-
-
-
cellobiose
-
40 mM, 22% inhibition
cellwall inhibitor of beta-fructosidase
Q43866
2.6 A crystal structure of cell-wall invertase 1 in complex with protein inhibitor, cell-wall inhibitor of beta-fructosidase, from tobacco. The structure recognizes a small amino acid motif in the inhibitor that directly targets the invertase active site. The activity of INV1 and its interaction with inhibitor are strictly pH-dependent with a maximum at about pH 4.5. At this pH, isothermal titration calorimetry reveals that the inhibitor tightly binds its target with nanomolar affinity
-
Co2+
-
strong inhibition
Co2+
-, Q564C7
38% inhibition at 1 mM
Co2+
-
2 mM, 64% residual activity
CTP
-
inhibition of neutral invertase, no inhibition of alkaline invertase
Cu2+
-
1 mM, 90% inhibition
Cu2+
-
1.7 mM, partial inhibition; CuSO4
Cu2+
-
1 mM, 75% inhibition of beta-fructofuranosidase activity, no effect on invertase activity
Cu2+
-
strong inhibition
Cu2+
-
1 mM, 53% inhibition of isozyme IT I, 85% inhibition of isozyme IT II
Cu2+
-, Q564C7
complete inhibition at 1 mM
Cu2+
-
significant inhibition at 0.04 mM
Cu2+
-
53% residual activity at 10 mM; 94% residual activity at 1 mM
Cu2+
Q2PCS4, -
virtually abolishes invertase activity
Cu2+
A1A2J8, -
10 mM, 52% residual activity
Cu2+
-
2 mM, 5% residual activity
CuSO4
-
complete inhibition at 0.5 mM
CuSO4
-
0.1 mM, 46% inhibition; 1 mM, 100% inhibition
CuSO4
-
6.2 mM, 50% inhibition
CuSO4
-
acid invertase is inhibited about 50% by 4.8 mM CuSO4. Alkaline invertase is inhibited by 0.6 mMCuSO4 up to 50%
D-fructose
-
product inhibition, 2.5 mM, 62% inhibition of isozyme IT I, 70% inhibition of isozyme IT II
D-fructose
-
competitive inhibition of the fruit acid invertase
D-fructose
-
competitive inhibition of the tuber and leaf enzyme
D-glucose
-
product inhibition, 2.5 mM, 58% inhibition of isozyme IT I, 72% inhibition of isozyme IT II
D-glucose
-
non-competitive inhibition of the tuber and leaf acid invertase
DTNB
-
0.5 mM, 24% inhibition of isozyme IT I, 11% activation of isozyme IT II
EDTA
-
slight inhibition
EDTA
-
12.34% residual activity at 0.2% (w/v)
endogenous protein inhibitors
-
-
-
endogenous protein inhibitors
Q43799
-
-
endogenous protein inhibitors
P49175
-
-
Fe2+
-
1 mM, 9% inhibition of beta-fructofuranosidase activity, 7% inhibition of invertase activity
-
Fe2+
-
strong inhibition
-
Fe2+
-, Q564C7
55% inhibition at 1 mM
-
Fe3+
-
2 mM, 0.8% residual activity
-
FeSO4
-
25.29% residual activity at 0.5 mM
fructose
-
slight
fructose
-
partial competitive
fructose
-
competitive inhibition of a classical type
fructose
-
competitive inhibitor of neutral and alkaline enzyme
fructose
-
competitive inhibition of a classical type
fructose
-
40 mM, 52% inhibition; 4 mM, 22% inhibition
fructose 1,6-diphosphate
-
-
fructose 6-phosphate
-
slight inhibition
fructoside
-
-
galactose
-
50 mM, 10% inhibition
glucose
-
non-competitive
glucose
-
non-competitive
glucose
-
non-competitive inhibitor of neutral and alkaline invertase
glucose
-
40 mM, 100% inhibition
GTP
-
inhibition of neutral invertase, no inhibition of alkaline invertase
guanidinium hydrochloride
-
2 M, 2% residual activity
Heptamolybdate
-
-
Hg+
-
complete inhibition at 10 mM
Hg2+
-
0.002 mM, complete inhibition; HgCl2
Hg2+
-
44% inhibition at 0.01 mM, 96% inhibition at 1 mM
Hg2+
-
0.002 mM, 55% inhibition of invertase I, complete inhibition of invertase II
Hg2+
-
1 mM, 67% inhibition
Hg2+
-
no inhibition of neutral and alkaline invertase
Hg2+
-
10 mM HgCl2
Hg2+
-
65% inhibition at 0.001 mM
Hg2+
-
invertase I is more than 2fold more resistant than invertase IIA and IIB
Hg2+
-
1.7 mM HgCl2, complete inhibition
Hg2+
-
HgCl2
Hg2+
-
1 mM, complete inhibition
Hg2+
-
1 mM, complete inhibition of beta-fructofuranosidase activity, 65% inhibition of invertase activity
Hg2+
-
strong inhibition
Hg2+
-
1 mM, 93% inhibition of isozyme IT I, 95% inhibition of isozyme IT II
Hg2+
A1A2J8, -
10 mM, no residual activity
Hg2+
-
2 mM, no residual activity
HgCl2
-
0.1 mM, 48% inhibition; 1 mM, 89% inhibition
HgCl2
-
does not affect acid invertase activity, 0.05 mM HgCl2 inhibits alkaline invertase activity by about 50%
I2
-
extracellular enzyme
I2
-
0.004 mM, complete inhibition
I2
-
0.004 mM, complete inhibition of invertase II, 9% inhibition of invertase I
invertase inhibitor of Solanum lycopersicum
-
6.7 nM tomato invertase with 1-2.5 nM inhibitor retains more than 90% invertase activity, 3-4 nM less than 90% activity, 5 nM about 75%, about 6.7 nM about 60% activity, pH 4.9, 37 degrees Celsius, 100 mM sucrose as substrate, proteinaceous functional inhibitor is expressed in leaves, flowers, and green fruit, proteolytically cleaving the invertase
-
INVINH1
-
endogenous tomato invertase inhibitor. Ectopic overexpression of INVINH1 in Arabidopsis thaliana specifically reduces cell wall invertase activity. By contrast, silencing its expression in tomato significantly increases the activity of cell wall invertase without altering activities of cytoplasmic and vacuolar invertases. Elevation of cell wall invertase activity in RNA interference transgenic tomato leads to a prolonged leaf life span involving in a blockage of abscisic acid-induced senescence and an increase in seed weight and fruit hexose level
-
iodoacetamide
-
5 mM, about 20% inhibition of isozyme IT I, 44% inhibition of isozyme IT II
K+
-
strong inhibition
K+
-
2 mM, 79% residual activity
K2PtCl3
-
non-competitive
KCl
-
70% residual activity at 0.5 mM
lactose
-
40 mM, 17% inhibition
Lectins
-
lectins which bind mannose and glucose, and those which bind oligomers of N-acetylglucosamine
-
Maleic acid
-
-
maltose
-
80 mM, 40% inhibition
Man-alpha(1-6)-(Xyl-beta(1-2))-Man-beta(1-4)-GlcNAc-beta(1-4)-Fuc-alpha(1-3)-GlcNAc
-
pineapple stem bromelain glycopeptide MUXF, complete inhibition
melezitose
-
40 mM, 10% inhibition
melibiose
-
40 mM, 13% inhibition
Mg2+
-
MgCl2, inhibition of alkaline invertase no inhibition of neutral invertase
Mg2+
-
slight inhibition
Mg2+
-
1 mM, 11% inhibition of isozyme IT I, 14% inhibition of isozyme IT II
Mg2+
-
79% residual activity at 10 mM
Mn2+
-
MnCl2, inhibition of alkaline invertase no inhibition of neutral invertase
Mn2+
-
1 mM, 1% inhibition of fructofuranosidase activity
Mn2+
-
strong inhibition
MnSO4
-
96.12% residual activity at 0.5 mM
Mo7O246-
-
-
-
MoCl2
-
1 mM
-
Na+
-
strong inhibition
Na+
-
73% residual activity at 10 mM
Na+
Q2PCS4, -
negatively effected by 0.8 M Na+
NaCl
-
0.1 M, partial, intracellular enzyme
NEM
-
10 mM, complete inhibition
NH4Cl
-
25% residual activity at 10 mM
Nicotiana tabacum cell wall inhibitor of fructosidase
-
cell-wall invertase: 28% activity of control; vacuolar invertase: 59% activity of control
-
Nicotiana tabacum vacuolar inhibitor of fructosidase
-
cell-wall invertase: 52% activity of control
-
Nornicotine
-
-
p-mercuribenzoate
-
-
Pb2+
-
strong inhibition
PCMB
-
0.1 mM, 94% inhibition of the CM-cellulose adsorbed enzyme, enzyme embedded within a polyacrylamide gel is inhibited 16.7%
PCMB
-
0.1 mM, significant inhibition
PCMB
-
0.002 mM, 21% inhibition of invertase I, 79% inhibition of invertase II
PCMB
-
6 mM, complete inhibition
PCMB
-
10 mM, 90% inhibition
PCMB
-
1 mM, 80% inhibition of beta-fructofuranosidase activity, 65% inhibition of invertase activity
PMSF
-
0.5 mM, 20% inhibition of isozyme IT I, 60% inhibition of isozyme IT II
polyethylene ethanol
-
65.48% residual activity at 0.5% (v/v)
protein C/VIF-RP
-
i.e. vacuole inhibitor protein of beta-fructosidase related proteins, amino acid sequence comparison
-
protein C/VIF-RP1-4
-
i.e. vacuole inhibitor protein of beta-fructosidase related proteins, 4 different amino acid sequences
-
protein CIF
-
i.e. cell wall inhibitor protein of beta-fructosidase, endogenous, specific inhibitor of the invertase, DNA and amino acid sequence determination and analysis, dendrogram, properties, overview
-
protein CIF
Chenopodium rubrum
-
i.e. cell wall inhibitor protein of beta-fructosidase, endogenous, specific inhibitor of the invertase, high expression in cell culture
-
protein CIF
-
i.e. cell wall inhibitor protein of beta-fructosidase, endogenous, specific inhibitor of the invertase
-
protein CIF
-
i.e. cell wall inhibitor protein of beta-fructosidase, endogenous, specific inhibitor of the invertase, high expression in cell culture
-
protein CIF
-
i.e. cell wall inhibitor protein of beta-fructosidase, endogenous, nonglycosylated, specific inhibitor of the invertase, DNA and amino acid sequence determination and analysis, strong expression in flowers, in ovary, stamen, and petals, co-expression with the enzyme in leaves and cell suspension culture, properties, expression regulation in response to abscisic acid and polyethylene glycol, overview
-
protein VIF
-
i.e. vacuole inhibitor protein of beta-fructosidase, endogenous, specific inhibitor of the invertase, DNA and amino acid sequence determination and analysis, dendrogram, properties, overview
-
protein VIF
-
i.e. vacuole inhibitor protein of beta-fructosidase, endogenous, specific inhibitor of the invertase
-
protein VIF
-
i.e. vacuole inhibitor protein of beta-fructosidase, endogenous, specific inhibitor of the invertase, DNA and amino acid sequence determination and analysis, strong expression in roots, properties, overview
-
pyridoxal
-
-
pyridoxal
-
1 mM, 15% inhibition
pyridoxal
-
1 mM, 65% inhibition of invertase I, 25% inhibition of invertase II
pyridoxal
-
invertase I is more than 2fold more resistant than invertase IIA and IIB
pyridoxal 5'-phosphate
-
-
pyridoxal hydrochloride
-
-
pyridoxamine
-
-
pyridoxamine
-
10 mM, 85% inhibition
pyridoxamine
-
10 mM, 28% inhibition of invertase I, 65% inhibition of invertase II
pyridoxine
-
-
pyridoxine
-
2.5 mM, 24% inhibition
pyridoxine
-
2.5 mM, 70% inhibition of invertase I, 20% inhibition of invertase II
pyridoxine
-
5 mM, 48% inhibition of isozyme IT I, 75% inhibition of isozyme IT II
pyridoxine hydrochloride
-
-
SDS
-, Q564C7
complete inhibition at 1 mM
Sodium dodecyl sulfate
A1A2J8, -
10 mM, no residual activity
sodium metasilicate
-
-
Sodium sulfate
-
0.1 M, partial, intracellular enzyme
Sorbitol
-
inhibitory action at 30 and 40% solution
Sucrose
-
substrate inhibition above 100 mM
Sucrose
-
substrate inhibition, 400 mM: 10% inhibition of isozyme IT I, 20% inhibition of isozyme IT II, 1 M: 10% inhibition of isozyme IT I, 62% inhibition of isozyme IT II
Sucrose
-
the intact enzyme is inhibited at sucrose concentrations in excess of 0.1 M
Sucrose
Q0ZR36
increase of the sucrose concentration from 2.5 mM to 1 M results in decreased hydrolysis activity
Sucrose
C0LIF8
substrate inhibition, but retains 50% activity up to 1 M sucrose
trans-aconitic acid
-
-
trehalose
-
50 mM, 23% inhibition
Tris
-
10 mM, 87% inhibition; 1 mM, 74% inhibition
Tris hydrochloride
-
-
Tris-HCl
-
Tris-HCl does not affect acid invertase activity up to 14 mM, 10 mM Tris-HCl inhibits alkaline invertase activity by about 50%
turanose
-
20 mM, complete inhibition
Tween 80
-
46.76% residual activity at 0.2% (v/v)
Urea
-
2 M, 29% residual activity
UTP
-
inhibition of neutral invertase, no inhibition of alkaline invertase
xylitol
-
inhibitory action at 35 and 40% solution
Zn2+
-
slight inhibition
Zn2+
-
1 mM ZnCl2
Zn2+
-
1 mM, 26% inhibition of beta-fructofuranosidase activity, 13% inhibition of invertase activity
Zn2+
-
slight inhibition
Zn2+
-
1 mM, no inhibition of isozyme IT I, 86% inhibition of isozyme IT II
Zn2+
-, Q564C7
56% inhibition at 1 mM
Zn2+
-
19% residual activity at 10 mM
Zn2+
Q2PCS4, -
slightly inhibitory
Zn2+
-
2 mM, 0.8% residual activity
ZnCl2
-
0.1 mM, 84% inhibition; 1 mM, 100% inhibition
ZnSO4
-
65.6% residual activity at 0.5 mM
Monoiodoacetic acid
-
-
additional information
-
product inhibition does not appear to be involved in the physiological regulation of the enzyme
-
additional information
-
high substrate concentrations and also high levels of the cleavage products glucose and fructose do not severly inhibit sucrose inversion
-
additional information
-
repression of the wild-type and mutant enzymes by D-glucose
-
additional information
-
no inhibition of the fruit acid invertase by D-glucose
-
additional information
-
sucrose specifically suppresses the expression of acid invertase Cin5
-
additional information
-
Pb2+ and Hg2+ do not inhibit the enzyme activity at a concentration of 1 mM
-
additional information
-
not inhibited by metal ions
-
additional information
Q2PCS4, -
Mg2+ does not affect the activity
-
additional information
-
vacuolar invertase: no inhibition of Arabidopsis thaliana cell wall/vacuolar fructosidase 1
-
additional information
-
MgCl2 and CaCl2 (1 mM) are typical alkaline invertase inhibitors, they do not inhibit the neutral invertase
-
additional information
-
decrease of enzyme activity during fruit development; decrease of enzyme activity in leaves during fruit development
-
additional information
-
not inhibitory: HgCl2
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
abscisic acid
P49175
water stress and abscisic acid activate the vacuolar isozyme and rapidly induce the expression of gene Ivr2 encoding the vacuolar isozyme in leaves and roots, in addition to accumulation of hexoses, glucose supply or polyethylene glycol at 1% do not directly activate the isozyme but induce its expression
Alkaline phosphatase
-
336 mM, 27% inhibition
-
Arabidopsis thaliana cell wall/vacuolar inhibitor of fructosidase 2
-
122% activation of vacuolar invertase
-
Beta vulgaris cell wall/vacuolar inhibitor of fructosidase
-
135% activation of vacuolar inveraase
-
beta-Lactoglobulin
-
336 mM, 27% inhibition
-
Bovine serum albumin
-
stimulates intracellular enzyme
-
Bovine serum albumin
-
activates
-
Bovine serum albumin
-
activates
-
Bovine serum albumin
-
activates
-
Bovine serum albumin
-
activates
-
Bovine serum albumin
-
activates isozyme IT II best at 0.2 mg/ml, and isozyme IT I slightly at 100 mg/ml
-
Bovine serum albumin
-, Q564C7
41% activation at 1 mM
-
Carboxymethylcellulose
-
33.17% increase of activity at 0.5 mM
-
cis-(+)-abscisic acid
-
-
concanavalin A
-
activates isozyme IT II best at 0.9 mg/ml
-
Cys
-
1-10 mM, activates
D-glucose
-
stimulates activity 2.68fold at 40 mM
D-sorbitol cinnamic ester
-
intense hydrophobic interactions between cinnamoyl groups of the compound with related groups of the enzyme immobilizes the enzyme and enhances substrate affinity and hydrolysis rate, immobilization at pH 5.5, 4°C, 12 h
-
deoxycholic acid
A1A2J8, -
10 mM, 137% of initial activity activity
Dextran sulfate
-
-
-
dithiothreitol
-
1 mM, 134% of initial activity
DTNB
-
0.5 mM, 11% activation of isozyme IT II, 24% inhibition of isozyme IT I
DTT
-, Q564C7
26% activation at 1 mM
EDTA
-
10 mM, 47% increase in activity
EDTA
-, Q564C7
32% activation at 1 mM
EDTA
-
slightly stimulating at 1 mM
EDTA
Q2PCS4, -
slight activity increase
heparin
-
activates
Human gamma-globulin
-
336 mM, 27% inhibition
-
iodoacetamide
-
enhances activity
K2HPO4
-
0.05% (w/v) KH2PO4 stimulates the production of FFase to 18%
lectin
-
lectins specific for alpha-mannose or alpha-glucose, activate. Lectins specific for N-acetylglucosamine oligomers, N-acetylgalactosamine or galactose do not activate
-
Nicotiana tabacum vacuolar inhibitor of fructosidase
-
154% activation of vacuolar invertase
-
ovalbumin
-
activates
-
Polyvinylpyrrolidone
-
synthetic polymer 2% PVP K 30
Polyvinylpyrrolidone
-
activates
reduced glutathione
-
activates
Triton X-100
A1A2J8, -
10 mM, 138% of initial activity activity
Tween-80
A1A2J8, -
10 mM, 138% of initial activity activity
urease
-
activates isozyme IT II best at 0.1 mg/ml
-
MgSO4
-
119.76% increase of activity at 0.5 mM
additional information
-
wounding and pathogen attack induces the enzyme
-
additional information
-
the cell wall-bound isozyme is induced by pathogen white blister rust, Albugo andida, attack on leaves, both the cell wall-bound and the vacuolar isozymes are up-regulated by hormone growth regulators, e.g. by gibberellin, GA4
-
additional information
Chenopodium rubrum
-
the cell wall-bound isozyme Cin1 is up-regulated by hormone growth regulators, e.g. by cytokinin
-
additional information
-
the cell wall-bound isozyme is rapidly induced by pathogen Erwinia carotovora attack on roots and leaves
-
additional information
Q43799
the cell wall-bound isozyme is induced by infection of leaves with the potato virus Y, and is up-regulated by hormone growth regulators, e.g. by abscisic acid
-
additional information
Q8L897
the enzyme is up-regulated by hormone growth regulators, e.g. by gibberellic acid, GA3
-
additional information
-
the cell wall-bound isozyme Lin6 is up-regulated by hormone growth regulators, e.g. by cytokinin
-
additional information
-
the vacuolar isozyme is induced by cold, temperature 1-4C, in tubers during storage
-
additional information
-
abscisic acid specifically activates both cell wall-bound and soluble acid invertases in vivo in a pH-dependent manner in developing grape berry leading to accumulation in crop sink organs, no effect by abscisic acid analogues (-)-ABA and trans-ABA, overview, the activation is affected positively by Ser/Thr protein kinase K252a, staurosporine, H7, and acid phosphatase, negatively by quercetin, overview
-
additional information
-
plant hormones abscisic acid and gibberellic acid induce cell wall isozyme CIN2 in peduncles, drought stress highly induces the cell wall-bound isozyme CIN2 7fold, and the vacuolar isozyme VIN2
-
additional information
-
brassinisteroids specifically induce the cell wall-bound isozyne CW in suspension cultured cells
-
additional information
-
induction of gene Irv2 in response to water stress in leaves
-
additional information
-
not activated by trans-abscisic acid
-
additional information
-
increase of enzyme activity in mesocarp tissue leaves during fruit development
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.3
-
1-kestose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50C
1.7
-
1-kestose
A1A2J8, -
pH 5.7, 37C
4.5
-
1-kestose
B8YJM2
pH 5.5, 42°C, alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose
49
-
1-kestose
-
pH 5.0, 37C
700
-
1-kestose
-
in 50 mM sodium acetate buffer (pH 5.0), at 30C
2.2
-
fructosylnystose
A1A2J8, -
pH 5.7, 37C
2.66
-
inulin
-
in sodium acetate buffer, 100 mM, pH 4.5, at 60C
7
-
inulin
-
mutant N254A, pH 5.0
9
-
inulin
-
mutant Y462A, pH 5.0
12
-
inulin
-
wild-type, pH 5.0
14
-
inulin
-
mutant Q435A, pH 5.0
15.6
-
inulin
-
pH 5.0, 37C
24
-
inulin
-
mutant Q176E, pH 5.0
25.9
-
inulin
A1A2J8, -
pH 5.7, 37C
44
-
inulin
-
mutant S281I, pH 5.0
47
-
inulin
-
mutant Q228V, pH 5.0
60
-
inulin
-
mutant N142Y, pH 5.0
575
-
inulin
-
mutant Q176S, pH 5.0
1.7
-
neokestose
A1A2J8, -
pH 5.7, 37C
1.6
-
nystose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50C
3.8
-
nystose
-
mutant Q435A, pH 5.0
3.9
-
nystose
A1A2J8, -
pH 5.7, 37C
8
-
nystose
-
mutant N142Y, pH 5.0; mutant N254A, pH 5.0; mutant Y462A, pH 5.0
8.1
-
nystose
-
mutant Q176E, pH 5.0
9
-
nystose
-
mutant Q228V, pH 5.0
11
-
nystose
-
mutant Q176S, pH 5.0
15
-
nystose
-
mutant S281I, pH 5.0
15
-
nystose
-
pH 5.0, 37C
1.3
-
raffinose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50C
1.6
-
raffinose
-
invertase I
2.9
-
raffinose
-
invertase I
4
-
raffinose
-
-
4.5
-
raffinose
-
pH 5.0, 40C
7.37
-
raffinose
-
in sodium acetate buffer, 100 mM, pH 4.5, at 60C
7.6
-
raffinose
-
enzyme form F-1
9.3
-
raffinose
-
-
10.6
-
raffinose
-
beta-FFase L, pH 5.1
10.8
-
raffinose
-
invertase II
13
-
raffinose
-
invertase III
14.2
-
raffinose
-
-
15
-
raffinose
-
-
16.7
-
raffinose
-
enzyme form F-2
17
-
raffinose
-
-
17
-
raffinose
-
invertase II
19.6
-
raffinose
-
soluble enzyme
20.4
-
raffinose
-
beta-FFase E, pH 5.1
20.7
-
raffinose
-
pH 7.5, alkaline invertase
21.7
-
raffinose
-
beta-FFase L, pH 3.6
32.19
-
raffinose
Q9ZTX2
pH 5.0, 37C
46.1
-
raffinose
-
-
125
-
raffinose
-
beta-FFase E, pH 3.6
150
-
raffinose
-
exteracellular enzyme
150
-
raffinose
-
extracellular and intracellular enzyme
150
-
raffinose
-
-
392
-
raffinose
-
pH 5.0, 37C
14
-
stachyose
-
invertase I
18.8
-
stachyose
-
-
25
-
stachyose
-
-
25
-
stachyose
-
invertase II
0.0625
-
Sucrose
-
-
0.29
-
Sucrose
-
-
0.32
-
Sucrose
-
neutral invertase
0.56
-
Sucrose
-
saline-released acid invertase
0.6
-
Sucrose
-
-
0.65
-
Sucrose
-
invertase 1
0.65
-
Sucrose
-
native enzyme
0.98
-
Sucrose
-
invertase 2
1
-
Sucrose
-
invertase IIA
1
-
Sucrose
-
invertase I
1.33
-
Sucrose
-
-
1.4
-
Sucrose
-
enzyme embedded within a polyacrylamide gel
1.43
-
Sucrose
-
deglycosylated enzyme
1.54
-
Sucrose
-
pH 4.7, 30C, F-form, extracellular
1.7
-
Sucrose
-
cell-wall bound enzyme
1.7
-
Sucrose
-
invertase IIB
1.8
-
Sucrose
-
acid invertase, pH 6.5, 37C
2
-
Sucrose
-
pH 4.7, 30C, S-form, extracellular
2
-
Sucrose
Q0ZR36
in 50 mM acetate buffer (pH 5.0) at 37C
2.1
-
Sucrose
-
invertase P-2
2.17
-
Sucrose
-
beta-FFasse-E, pH 5.1
2.4
-
Sucrose
-
invertase I
2.4
-
Sucrose
-
pH 7.0, 37C
2.4
-
Sucrose
-
wild-type, pH 5.0
2.46
-
Sucrose
C0LIF8
pH 7.0, 50C
2.5
-
Sucrose
-
extracellular enzyme, pH 4.5, 60C
2.8
-
Sucrose
-
acid invertase
3
3.5
Sucrose
-
native invertase, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
3.3
-
Sucrose
-
-
3.33
-
Sucrose
-
pH 4.5, 30C, isoenzyme AIV I
3.45
-
Sucrose
-
beta-FFase E, pH 4.5
3.5
-
Sucrose
-
soluble enzyme
3.5
-
Sucrose
-
pH 5.0, 40C
3.57
-
Sucrose
-
in MES buffer, pH 5.0, at 40C
3.7
-
Sucrose
-
beta-FFase L, pH 5.1
3.7
-
Sucrose
-
pH 5.0, 37C, isozyme IT II
3.8
4.4
Sucrose
-
acid invertases
4
-
Sucrose
B8YJM2
pH 5.5, 42°C, alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose
4.2
-
Sucrose
-
-
4.2
-
Sucrose
-
-
4.4
-
Sucrose
-
invertase P-1
4.4
-
Sucrose
-
invertase II
4.41
-
Sucrose
-
pH 6.5, 37C, hyperbolic saturation kinetics
4.58
-
Sucrose
-
pH 4.5, 30C, isoenzyme AIV II
4.76
-
Sucrose
-
beta-FFase L, pH 4.5
4.89
-
Sucrose
-
-
4.9
-
Sucrose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50C
4.97
-
Sucrose
Q94C05, Q94C06
pH 5.0, recombinant isozyme lbbetafruct3
5
-
Sucrose
-
enzyme forms INV1 and INV3
5
-
Sucrose
-
mutant Q176S, pH 5.0
5.3
-
Sucrose
-
-
5.4
-
Sucrose
-
-
5.8
-
Sucrose
O81083
pH 5.6, 28°C, wild-type
5.89
-
Sucrose
-
beta-FFase L, pH 3.6
6.2
-
Sucrose
-
enzyme form F-1
6.4
-
Sucrose
-
invertase II
6.4
-
Sucrose
-
wild-type, pH 5.0
6.6
-
Sucrose
-
invertase III
6.7
-
Sucrose
-
invertase II
6.9
-
Sucrose
-
enzyme adsorbed on CM-cellulose
7
-
Sucrose
-
mutant Q176E, pH 5.0; mutant Y462A, pH 5.0
7.2
-
Sucrose
-
invertase entrapped into calcium alginate beads
7.43
-
Sucrose
-
immobilized enzyme, pH 6.0, 45C
7.69
-
Sucrose
-
beta-FFase E, pH 3.6
8
-
Sucrose
-
mutant N254A, pH 5.0
8.1
-
Sucrose
-
invertase I
8.7
-
Sucrose
-
-
8.8
-
Sucrose
O81083
pH 5.6, 28°C, W440Y
9
-
Sucrose
-
mutant Q435A, pH 5.0
9.1
-
Sucrose
-
free enzyme, pH 5.0, 30C
10
-
Sucrose
-
-
10.05
-
Sucrose
-
25% xylitol, 55°C, pH 4.5
10.1
-
Sucrose
Q94C05, Q94C06
pH 5.0, recombinant isozyme lbbetafruct2
10.69
-
Sucrose
Q9ZTX2
pH 5.0, 37C
10.75
-
Sucrose
-
30% glycerol, 55°C, pH 4.5
10.9
-
Sucrose
-
pH 7.0, 37C, isozyme IT I
11
-
Sucrose
-
mutant Q228V, pH 5.0
11.5
-
Sucrose
-
15% sorbitol, 55°C, pH 4.5
11.9
-
Sucrose
-
enzyme form F-2
12
-
Sucrose
-
enzyme form INV2
12.3
-
Sucrose
-
extracellular invertase I
12.5
-
Sucrose
-
native invertase, 55°C, pH 4.5
13
-
Sucrose
-
mutant S281I, pH 5.0
13.4
-
Sucrose
-
in sodium acetate buffer, 100 mM, pH 4.5, at 60C
13.6
-
Sucrose
-
pH 5.0
14
-
Sucrose
-
85C, pH 6.0
14.2
-
Sucrose
-
-
14.3
-
Sucrose
-
pH 6.8, neutral invertase
15.1
-
Sucrose
-
pH 8.0, alkaline invertase
16.66
-
Sucrose
-
40% xylitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
17.85
-
Sucrose
-
40% glycerol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
18.7
-
Sucrose
-
pH 7.5, neutral invertase
19
-
Sucrose
-
mutant N24S, pH 4.8, 50C
19.23
-
Sucrose
-
40% glycerol, 55°C, pH 9
20
-
Sucrose
-
neutral and alkaline invertase
21
-
Sucrose
-
pH 4.5
21
-
Sucrose
-
37°C
21.27
-
Sucrose
-
40% sorbitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
21.73
-
Sucrose
-
40% xylitol, 55°C, pH 9
24.5
-
Sucrose
-
free enzyme, pH 4.5, 57C
25
26
Sucrose
-
extracellular enzyme
25
-
Sucrose
-
extracellular enzyme
25.6
-
Sucrose
-
isoforms EINV1, EIV2, EINV3, EINV4, pH 4.5, 25C
26
-
Sucrose
-
extracellular enzyme
27.1
-
Sucrose
Q2PCS4, -
in 100 mM Na-acetate, pH 4.6, and 100 mM sucrose, at 35C
29
-
Sucrose
-
mutant N142Y, pH 5.0
29.41
-
Sucrose
-
native invertase, 55°C, pH 9
33.3
-
Sucrose
-
-
37.6
-
Sucrose
-
immobilized enzyme, pH 4.5, 57C
38
-
Sucrose
A1A2J8, -
pH 5.7, 37C
38
-
Sucrose
-
wild-type, pH 4.8, 50C
38.6
-
Sucrose
-
alkaline invertase, pH 6.5, 37C
41.2
-
Sucrose
-
free enzyme
42
-
Sucrose
-
pH 7.0, 30C
45
-
Sucrose
-
pH 5.5
46.5
-
Sucrose
-
enzyme immobilized on polyurethane rigid adhesive foam, pH 5.0, 50C
51
-
Sucrose
-
pH 5.5, 30°C, free chimeric fusion enzyme INVA-CBD
53.68
-
Sucrose
-
pH 5.0, 37°C, wild-type enzyme
57
-
Sucrose
Corynebacterium murisepticum
-
-
61.2
-
Sucrose
-
free enzyme, pH 5.0, 50C
67
-
Sucrose
-
pH 6.0
70.4
-
Sucrose
-
covalently bound enzyme, pH 5.0, 30C
78
-
Sucrose
-
immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5
87
-
Sucrose
-
pH 4.0
98
-
Sucrose
-
free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5
110
-
Sucrose
-
pH 5.5, 30°C, free INVA
130
-
Sucrose
-
adsorbed enzyme, pH 5.0, 30C
140.7
-
Sucrose
-
pH 5.0, 37°C, mutant enzyme C317A
142.6
-
Sucrose
-
pH 5.0, 37°C, mutant enzyme E316Q
153
-
Sucrose
-
pH 5.5, 30°C, cellulose immobilized chimeric fusion enzyme INVA-CBD
160
-
Sucrose
-
pH 5.0, 37C
160.6
-
Sucrose
-
pH 5.0, 50°C, 55 mg/ml
169.8
-
Sucrose
-
pH 5.0, 37°C, mutant enzyme W159F
189
-
Sucrose
-
pH 5.5, 30°C, Nylon-6 immobilized INVA
213.6
-
Sucrose
-
pH 5.0, 37°C, mutant enzyme W159L
227
-
Sucrose
-
pH 4.5, 35C
230
-
Sucrose
-
in 50 mM sodium acetate buffer (pH 5.0), at 30C
398
-
Sucrose
-
pH 5.0, 37C
1300
-
Sucrose
-
mutant N21S, pH 4.8, 50C
5000
-
Sucrose
-
mutant W19Y/N21S, pH 4.8, 50C
0.5
-
levan
-
pH 5.0, 37C
additional information
-
additional information
-
Km-values of soluble and micelle entrapped enzyme
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
kinetics, isozymes SAI and CWI, isozyme Sai has a lower KM for sucrose and a higher KM for raffinose compared to isozyme CWI
-
additional information
-
additional information
-
enzyme in cells immobilized in gelatin hydrogels are analyzed concerning kinetics, thermostability, and reusability, overview
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.5
-
1-kestose
B8YJM2
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranosyl-(1,2)-beta-D-fructofuranose
26.3
-
1-kestose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50C
64
-
1-kestose
-
pH 5.0, 37C
107
-
1-kestose
A1A2J8, -
pH 5.7, 37C
75.4
-
fructosylnystose
A1A2J8, -
pH 5.7, 37C
2
-
inulin
-
mutant S281I, pH 5.0
3
-
inulin
-
mutant Q228V, pH 5.0
5
-
inulin
-
mutant Y462A, pH 5.0
10
-
inulin
-
mutant Q435A, pH 5.0
12
-
inulin
-
mutant N254A, pH 5.0
25
-
inulin
-
wild-type, pH 5.0
39
-
inulin
-
mutant Q176E, pH 5.0
68
-
inulin
-
mutant N142Y, pH 5.0
77.4
-
inulin
A1A2J8, -
pH 5.7, 37C
106
-
inulin
-
pH 5.0, 37C
1710
-
inulin
-
mutant Q176S, pH 5.0
142.7
-
neokestose
A1A2J8, -
pH 5.7, 37C
2
-
nystose
-
mutant Q228V, pH 5.0
6
-
nystose
-
mutant S281I, pH 5.0
6.9
-
nystose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50C
22
-
nystose
-
mutant Y462A, pH 5.0
60
-
nystose
-
mutant N254A, pH 5.0
76
-
nystose
-
mutant Q435A, pH 5.0
87
-
nystose
-
mutant N142Y, pH 5.0
92
-
nystose
-
mutant Q176E, pH 5.0
95
-
nystose
-
pH 5.0, 37C
152.8
-
nystose
A1A2J8, -
pH 5.7, 37C
230
-
nystose
-
mutant Q176S, pH 5.0
10.9
-
raffinose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50C
16
-
raffinose
-
pH 5.0, 37C
140
-
raffinose
-
-
5.7
-
Sucrose
B8YJM2
alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose
20
-
Sucrose
-
mutant S281I, pH 5.0
21
-
Sucrose
-
pH 5.0, 50°C
33
-
Sucrose
-
mutant Q228V, pH 5.0
43
-
Sucrose
-
mutant Y462A, pH 5.0
57.86
-
Sucrose
-
pH 5.0, 37°C, mutant enzyme W159L
79.4
-
Sucrose
A1A2J8, -
pH 5.7, 37C
80.5
-
Sucrose
-
in 0.2M sodium acetate buffer (pH 5.6), at 50C
84
-
Sucrose
-
wild-type, pH 5.0
84
-
Sucrose
-
pH 5.0, 37C
85
-
Sucrose
-
mutant N254A, pH 5.0
88
-
Sucrose
-
mutant Q176E, pH 5.0
93.3
-
Sucrose
-
native invertase, 55°C, pH 9
99
-
Sucrose
-
mutant Q435A, pH 5.0
106
-
Sucrose
-
wild-type, pH 5.0
125
-
Sucrose
-
pH 7.0, 30C
127
-
Sucrose
-
pH 7.0, 37C
134
-
Sucrose
-
mutant Q176S, pH 5.0
154
-
Sucrose
-
native invertase, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
262
-
Sucrose
-
mutant N142Y, pH 5.0
338.4
-
Sucrose
-
pH 5.0, 37°C, mutant enzyme E316Q
360
-
Sucrose
-
mutant N24S, pH 4.8, 50C
501.7
-
Sucrose
-
pH 5.0, 37°C, wild-type enzyme
690.8
-
Sucrose
-
40% sorbitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
737.5
-
Sucrose
-
40% xylitol, 55°C, pH 9
1055
-
Sucrose
-
40% glycerol, 55°C, pH 9
1078
-
Sucrose
-
40% glycerol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
1232
-
Sucrose
-
40% xylitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5
1243
-
Sucrose
-
pH 5.0, 37°C, mutant enzyme C317A
1541
-
Sucrose
-
native invertase, 55°C, pH 4.5
1891
-
Sucrose
-
15% sorbitol, 55°C, pH 4.5
2148
-
Sucrose
-
30% glycerol, 55°C, pH 4.5
2342
-
Sucrose
-
pH 5.0, 37°C, mutant enzyme W159F
2428
-
Sucrose
-
25% xylitol, 55°C, pH 4.5
2600
-
Sucrose
-
-
3300
-
Sucrose
-
mutant W19Y/N21S, pH 4.8, 50C
3430
-
Sucrose
-
wild-type, pH 4.8, 50C
4000
-
Sucrose
-
mutant N21S, pH 4.8, 50C
12000
-
Sucrose
-
free enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5.5
50000
-
Sucrose
-
immobilized enzyme, 290 mM sucrose, room temperature, 50 mM sodium acetate buffer, pH 5
7300000
-
Sucrose
-
85C, pH 6.0
89
-
levan
-
pH 5.0, 37C
additional information
-
additional information
-
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
63.2
-
1-kestose
A1A2J8, -
pH 5.7, 37C
1063
34.2
-
fructosylnystose
A1A2J8, -
pH 5.7, 37C
245531
0.04
-
inulin
-
mutant S281I, pH 5.0
11722
0.06
-
inulin
-
mutant Q228V, pH 5.0
11722
0.5
-
inulin
-
mutant Y462A, pH 5.0
11722
0.7
-
inulin
-
mutant Q435A, pH 5.0
11722
1.1
-
inulin
-
mutant N142Y, pH 5.0
11722
1.6
-
inulin
-
mutant Q176E, pH 5.0
11722
1.7
-
inulin
-
mutant N254A, pH 5.0
11722
2.1
-
inulin
-
wild-type, pH 5.0
11722
3
-
inulin
A1A2J8, -
pH 5.7, 37C
11722
3
-
inulin
-
mutant Q176S, pH 5.0
11722
83.9
-
neokestose
A1A2J8, -
pH 5.7, 37C
79029
0.2
-
nystose
-
mutant Q228V, pH 5.0
14616
0.4
-
nystose
-
mutant S281I, pH 5.0
14616
2.7
-
nystose
-
mutant Y462A, pH 5.0
14616
7.5
-
nystose
-
mutant N254A, pH 5.0
14616
11
-
nystose
-
mutant N142Y, pH 5.0
14616
20
-
nystose
-
mutant Q435A, pH 5.0
14616
21
-
nystose
-
mutant Q176S, pH 5.0
14616
39.2
-
nystose
A1A2J8, -
pH 5.7, 37C
14616
51
-
nystose
-
mutant Q176E, pH 5.0
14616
1.5
-
Sucrose
-
mutant S281I, pH 5.0
16818
2.1
-
Sucrose
A1A2J8, -
pH 5.7, 37C
16818
3
-
Sucrose
-
mutant Q228V, pH 5.0
16818
6
-
Sucrose
-
mutant Y462A, pH 5.0
16818
9
-
Sucrose
-
mutant N142Y, pH 5.0
16818
10.6
-
Sucrose
-
mutant N254A, pH 5.0
16818
11
-
Sucrose
-
mutant Q435A, pH 5.0
16818
12.6
-
Sucrose
-
mutant Q176E, pH 5.0
16818
16.6
-
Sucrose
-
wild-type, pH 5.0
16818
26.8
-
Sucrose
-
mutant Q176S, pH 5.0
16818
35
-
Sucrose
-
wild-type, pH 5.0
16818
520000
-
Sucrose
-
85C, pH 6.0
16818
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
259
-
Sucrose
-
Nylon-6 immobilized INVA
268.1
-
Sucrose
Q0ZR36
in 50 mM acetate buffer (pH 5.0) at 37C
283
-
Sucrose
-
cellulose immobilized chimeric fusion enzyme INVA-CBD
313
-
Sucrose
-
free INVA
393
-
Sucrose
-
free chimeric fusion enzyme INVA-CBD
673
-
Sucrose
C0LIF8
pH 7.0, 50C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0003
-
-
value about, leaf, 20 days after anthesis
0.0005
-
-
inner membrane
0.0008
-
-
outer membrane
0.00083
-
-
value about, leaf, 12 and 16 days after anthesis; value about, leaf, 20 days after anthesis
0.00125
-
-
value about, leaf, 4 days after anthesis
0.00167
-
-
value about, leaf, 16 days after anthesis; value about, leaf, 2 days before anthesis and day of anthesis; value about, leaf, 8 days after anthesis
0.003
-
-
value about, leaf, 12 days after anthesis
0.0067
-
-
value about, leaf, 8 days after anthesis
0.0083
-
-
value about, leaf, 2 days before anthesis, day of anthesis and 4 days after anthesis
0.0116
-
-
matrix, 10 min, room temperature, control
0.013
-
-
VI
0.014
-
-
untreated mitochondria, 37°C
0.0196
-
-
matrix
0.02
-
-
soluble cell extract, in 100 mM sodium citrate buffer (pH 4.5)
0.0231
-
-
mitochondria, alamethicin treated to enable unrestricted transport, 37°C
0.0269
-
-
matrix, 10 min, 37°C
0.03
-
-
value about, mesocarp tissue, 20 days after anthesis
0.067
-
-
value about, mesocarp tissue, 16 days after anthesis
0.083
-
-
value about, mesocarp tissue, 8 and 12 days after anthesis
0.1
-
-
value about, mesocarp tissue, 2 days before anthesis
0.1167
-
-
value about, mesocarp tissue, 4 days after anthesis; value about, mesocarp tissue, day of anthesis
0.13
-
-
value about, mesocarp tissue, 2 days before anthesis and day of anthesis
0.143
-
-
CWI
0.15
-
-
value about, mesocarp tissue, 4 days after anthesis
0.1917
-
-
value about, mesocarp tissue, 20 days after anthesis; value about, mesocarp tissue, 8 days after anthesis
0.2083
-
-
value about, mesocarp tissue, 16 days after anthesis
0.225
-
-
value about, mesocarp tissue, 12 days after anthesis
0.768
-
-
pH 5.0, 37°C, mutant enzyme W159L with substrate sucrose, 33.2% activity with substrate raffinose compared to wild-type
0.82
-
-
pH 6.5, 37C, alkaline invertase
0.866
-
-
-
2.465
-
-
-
3.3
-
-
enzyme expressed in Hansenula polymorpha
3.4
-
-
enzyme expressed in Pichia pastoris
4.43
-
-
pH 5.0, 37°C, mutant enzyme E316Q with substrate sucrose, 15.5% activity with substrate raffinose compared to wild-type
4.7
-
-
extracellular enzyme, crude protein cell extract
6
-
-
crude extract, at pH 4.5, 60C
6.58
-
-
pH 5.0, 37°C, wild-type enzyme with substrate sucrose
11.5
-
-
intracellular enzyme, crude protein cell extract
12.3
-
-
after purification, in 100 mM sodium citrate buffer (pH 4.5)
16.3
-
-
pH 5.0, 37°C, mutant enzyme C317A with substrate sucrose, 20.7% activity with substrate raffinose compared to wild-type
16.6
-
-
pH 6.5, 37C, acid invertase
20.6
-
-
cellulose immobilized chimeric fusion enzyme INVA-CBD
25.6
-
-
free chimeric fusion enzyme INVA-CBD
26
-
-
purified isozyme IT II
28.8
-
-
purified isozyme IT I
30.73
-
-
pH 5.0, 37°C, mutant enzyme W159F with substrate sucrose, 41% activity with substrate raffinose compared to wild-type
39
-
-
free INVA
42.4
-
-
crude extract, at 30C
42.5
-
-
after 7.1fold purification, at pH 4.5, 60C
47.92
-
-
crude extract, at pH 5.0 and 40 ?C
48
-
-
-
50
-
-
invertase I
51.67
-
-
purified enzyme
56.67
-
-
enzyme form P-1
67
-
-
enzyme form P-2
70
-
Q94C05, Q94C06
purified recombinant isozyme lbbetafruct3
86
-
A1A2J8, -
pH 5.7, 37C
90
-
B8YJM2
palatinose as substrate
101
-
-
purified enzyme
109
-
-
invertase IIA
112.4
-
-
invertase I
140.4
-
-
-
158.2
-
-
purified native enzyme
186
-
-
immobilized enzyme, pH 6.0, 45C
192
-
-
invertase IIB
218
-
-
free enzyme, pH 6.0, 45C
220
-
B8YJM2
nystose as substrate
245.6
-
-
after 3.11fold purification, at pH 5.0 and 40 ?C
253.6
-
-
invertase II
420
-
-
isoenzyme P-1
455
-
-
isoenzyme P-2
456
-
-
Nylon-6 immobilized INVA
466.1
-
-
invertase III
553
-
Q94C05, Q94C06
purified recombinant isozyme lbbetafruct2
601
-
-
extracellular enzyme, pH 4.5, 60C
884.3
-
Q2XQ19, Q2XQ20, Q2XQ21
purified recombinant His-tagged isozyme betafruct2
1200
-
B8YJM2
1-kestose as substrate
1480
-
-
isoform EINV4, 25C, pH 4.5
1497
-
-
S-form
1695
-
Q2XQ19, Q2XQ20, Q2XQ21
purified recombinant His-tagged isozyme betafruct3
2180
-
-
after 51.4fold purification, at 30C
2410
-
-
isoform EINV2, 25C, pH 4.5
2470
-
-
isoform EINV3, 25C, pH 4.5
2560
-
-
F-form
2970
-
-
-
3128
-
-
intracellular enzyme
3160
-
-
isoform EINV1, 25C, pH 4.5
4700
-
-
intracellular enzyme
4800
-
-
extracellular enzyme
5200
-
B8YJM2
sucrose as substrate
51800
-
-
85C, pH 6.0
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
repressed and derepressed activities of wild-type and mutant enzymes in dry cell material, overview
additional information
-
P49175
-
additional information
-
-, Q564C7
-
additional information
-
-
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, reduced activity after feeding with sucrose observed
additional information
-
B8YJM2
no activity with substrates: maltose (alpha-D-glucopyranosyl-(1,4)-D-glucopyranose), lactose (beta-D-galactopyranosyl-(1,4)-D-glucopyranose), leucrose (alpha-D-glucopyranosyl-(1,5)-D-fructofuranose)
additional information
-
-
Lin8-RNAi line 33 activity: 3.4 micromol/squaremeter/min, vacuolar invertase: 3.6 micromol/squaremeter/min; Lin8-RNAi line 50 activity: 3.6 micromol/squaremeter/min, vacuolar invertase: 5.6 micromol/squaremeter/min; Lin8-RNAi line 57 activity: 1.5 micromol/squaremeter/min, vacuolar invertase: 4.0 micromol/squaremeter/min; wild-type activity: 40.5 micromol/squaremeter/min, vacuolar invertase: 4.2 micromol/squaremeter/min
additional information
-
-
mutant with 11.4% reduced neutral invertase activity compared to wild-type, with higher sucrose and lower glucose (-10% in leaves, -22% in roots) and fructose (-12% in leaves, -21% in roots) contents in 14-day-old mutant plants than in wild-type, part of the mutant phenotype is due to glucose deficit, mutant morphology phenotype is not fully expressed in 3% glucose medium
additional information
-
-
maximum activity at 15% sorbitol (1.23-fold increase), increased activity from 5-20% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5; maximum activity at 25% xylitol (1.57-fold increase), increased activity from 10-30% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5; maximum activity at 30% glycerol (1.39-fold increase), increased activity from 10-40% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
6
-
enzyme immobilized on bead DEAHP-cellulose
3
-
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct2
3.5
5
-
extracellular and intracellular enzyme
3.5
5
-
isoforms EINV1, EIV2, EINV3, EINV4
3.5
5.5
-
intracellular and extracellular enzyme
3.5
7.5
-
about 70% of maximal activity, pH 7.5: about 50% of maximal activity
3.5
-
-
invertase IIb
3.5
-
-
and a second optimum at pH 5.5
4
4.5
-
both extracellular and intracellular enzyme
4
5
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct3
4
7
-
pH 4.0: about 60% of maximal activity, pH 7.0: about 25% of maximal activity
4
-
-
extracellular enzyme
4
-
-
invertase 1
4
-
-
acid invertase isozyme IT II
4.3
-
-
invertase I
4.4
-
-
S-form and F-form of invertase
4.5
5.5
-
native enzyme
4.5
-
-
-
4.5
-
-
invertase Ib and IIa
4.5
-
-
hydrolysis of sucrose
4.5
-
-
soluble enzyme
4.5
-
-
invertase P-1
4.5
-
-
free enzyme and enzyme embedded within a polyacrylamide gel
4.5
-
-
heterologous invertase
4.5
-
-
pH-optimum
4.6
5
-
-
4.6
-
-
free enzyme and enzyme entrapped into calcium alginate beads
4.6
-
Q2PCS4, -
-
4.8
-
-
-
5
5.5
-
immobilized enzyme
5
-
-
invertase Ia
5
-
-
invertase P-2
5
-
-
invertase II
5
-
-
hydrolysis of sucrose and raffinose
5
-
Q9ZTX2
-
5
-
-
free enzyme, and covalently bound enzyme
5
-
-
assay at
5
-
Q94C05, Q94C06
recombinant isozyme lbbetafruct2; recombinant isozyme lbbetafruct3
5
-
Q0ZR36
-
5
-
-
immobilized enzyme, room temperature, 290 mM sucrose, pH 3.5-5.5: 50 mM sodium phosphate and 50 mM sodium acetate buffer, pH 6-7.5: 50 mM sodium acetate buffer
5
-
-
wild-type and mutant enzymes
5
-
-
recombinant truncated cytosolic protein
5.1
-
-
hydrolysis of raffinose
5.3
-
-
acid invertase
5.5
-
-
intracellular enzyme
5.5
-
-
cell-wall bound enzyme
5.5
-
-
and a second optimum at pH 3.5
5.5
-
-
recombinant enzyme
5.5
-
-
deglycosylated enzyme
5.5
-
O33833
assay at
5.5
-
-
assay at
5.5
-
B8YJM2
highest activity at pH 5.5-6.0
5.5
-
-
free enzyme, room temperature, 290 mM sucrose, pH 3.5-5.5: 50 mM sodium phosphate and 50 mM sodium acetate buffer, pH 6-7.5: 50 mM sodium acetate buffer
5.5
-
-
immobilized and free enzyme, INVA and chimeric fusion enzyme INVA-CBD, 30°C, pH 3.5-5.5: 50 mM acetate buffer, pH 6-7.5: 50 mM sodium citrate phosphate buffer, pH 7.5-8.5: 20 mM Tris-HCl buffer
5.6
-
-
invertase 2
6
-
-
fructofuranosidase and invertase activity
6
-
-
adsorbed enzyme
6
-
-
assay at, pH-optimum estimated for
6.5
-
C0LIF8
-
6.8
-
-
neutral invertase
6.9
-
Chenopodium rubrum
Q42691
assay at
7
7.5
-
alkaline invertase isozyme IT I
7
7.6
-
-
7
8
-
broad, free enzyme
7
-
Corynebacterium murisepticum
-
-
7.2
-
-
pH adjusted with KOH or HCl
7.3
-
-
alkaline invertase
8
-
-
alkaline invertase
additional information
-
-
the isozymes with different subcellular localization show different pH optima
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
4.5
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct2, about 90% of maximal activity at pH 2.0 and 20% at pH 4.5
2
6
-
pH 2.0: about 80% of maximal activity, pH 6.0: about 75% of maximal activity
2.7
5.7
-
pH 2.7: about 55% of maximal activity, pH 5.7: about 70% of maximal activity, hydrolysis of sucrose
3
5
-
pH 3.0: about 50% of maximal activity, pH 5.0: about 80% of maximal activity, invertase II
3
5.4
-
about 65% of maximal activity at pH 3.0 and at pH 5.4, cytoplasmic enzyme
3
6
-
pH 3.0: enzyme from seedlings shows 45% of maximal activity, enzyme from aged root slices shows about 30% of maximal activity, pH 6.0: about 30% of maximal activity
3
6
-
pH 3.0: about 40% of maximal activity of F-form of invertase, about 50% of maximal activity of S-form of invertase, pH 6.0. about 40% of maximal activity of F-form of invertase, about 75% of maximal activity of S-form of invertase
3
6.3
-
about 50% of maximal activity at pH 3.0 and pH 6.3, covalently bound enzyme
3
7
-
pH 3.0: about 90% of maximal activity, pH 7.0: about 30% of maximal activity
3
8
B8YJM2
highest activity at pH 5.5-6.0, more than 75% at pH 5, and 6.5-7, more than 50% at pH 4, about 25% at pH 8, about 10% at pH 3, substrate sucrose, buffers at 100 mM, pH 3-4: citric acid-sodium citrate, pH 4-7: Na2HPO4-NaH2PO4, pH 7-8: Tris-HCl
3.3
6
-
pH 3.3: about 50% of maximal activity of the free enzyme, about 70% of maximal activity of the enzyme embedded within a polyacrylamide gel, pH 6.0: about 10% of activity of the free enzyme, about 45% of activity of the enzyme embedded within a polyacrylamide gel
3.5
6
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct3, about 50% of maximal activity at pH 3.5 and pH 6.0
3.5
7.5
-
free enzyme: maximum activity at pH 5.5, about 70% activity at pH 5, about 40% activity at pH 6, activities below 20% at pH 4.5 and 7, no activity at pH 3-4 and 7.5; immobilized enzyme: maximum activity at pH 5, low activity at pH 5.5, about 50% activity at pH 4.5, no activity at pH 3-4 and 6-7.5
3.5
7.5
-
chimeric fusion enzyme INVA-CBD: highest activity at pH 5.5, about 80% retained in immobilized enzyme at pH 6, in free enzyme about 60%. Both types with less than 50% activity below pH 5 and above pH 6.5
3.5
8.5
-
INVA: about 50% activity retained between pH 4.5 and 6.5 in the immobilized enzyme, about 40% in the free enzyme, no activity remains at pH 8.5 in both forms, none in the free enzyme at pH 3.5, about 30% in the immobilized enzyme
3.7
5.5
-
about 75% of maximal activity, pH 5.5: about 50% of maximal activity
3.7
6.5
-
pH 3.7: about 35% of maximal activity, pH 6.5: about 50% of maximal activity
3.7
7
-
about 50% of maximal activity at pH 3.7 and pH 7.0, free enzyme
4
5
-
pH 4.0: about 70% of maximal activity, pH 5.0: about 55% of maximal activity, cell-wall bound enzyme
4
5
-
pH 4: about 70% of maximal activity, pH 5.0: about 60% of maximal activity
4
6
-
pH 4.0: about 40% of maximal activity, pH 6.0: about 20% of maximal activity
4
7.5
-
about 50% of maximal activity at pH 4.3 and pH 7.5, adsorbed enzyme
4
7.5
-
free enzyme
4
7.5
-
acid invertase
4
8
-
free enzyme
4
9.5
-
immobilized enzyme
4.2
7.1
-
more than 50% of maximal activity between pH 4.2 and pH 7.1
4.2
7.4
-
50% of maximal activity at pH 4.2 and at pH 7.4
4.5
6
-
about 70% of maximal activity at pH 4.5 and at pH 6.0
4.5
7
-
pH 4.5: about 35% of maximal activity, pH 7.0: about 45% of maximal activity
4.5
7.5
-
pH 4.5: about 50% of maximal activity of fructofuranosidase and invertase activity, pH 7.5: about 50% of maximal activity of invertase activity, about 40% of maximal activity of fructofuranosidase activity
4.5
8.5
-
active in the range
4.6
5
-
-
4.8
7.4
-
pH 4.8: about 55% of maximal activity, pH 7.4: about 40% of maximal activity, pollen wall enzyme
4.8
8
-
pH 4.8: about 35% of maximal activity, pH 8.0: about 60% of maximal activity, neutral invertase
5
6.5
-
pH 5.0: about 35% of maximal activity, pH 7.5: about 75% of maximal activity
5
8.5
-
less than 20% activity at pH 5, more than 80% activity between pH 6.2 and 7.7 with maximum at pH 7 and 7.2, 50-70% activity between pH 8 and 8.5
5
9
-
alkaline invertase
6
7.5
-
about 40% of maximal activty at pH 6.0 and at pH 7.5
7
9
-
pH 7.0: about 50% of maximal actiovity, pH 9.0: about 70% of maximal activity, alkaline invertase
additional information
-
-
pH profiles of free and immobilized enzymes
additional information
-
-
-
additional information
-
-
pH profile
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
26
-
Chenopodium rubrum
Q42691
assay at
30
40
-
both isozyme IT I and isozyme IT II
30
70
-
no defined optimum, soluble F-form
30
-
-
heterologous enzyme
30
-
-
assay at
30
-
-
immobilized and free enzyme, INVA and chimeric fusion enzyme INVA-CBD, 50 mM acetate buffer, pH 5.5
37
40
-
-
37
40
Q0ZR36
-
37
-
Corynebacterium murisepticum
-
-
37
-
O33833
assay at
37
-
-
both acid and alkaline invertase
40
-
-
invertase I
40
-
-
-
40
-
Q9ZTX2
-
40
-
Q2PCS4, -
slightly below 40C
40
-
-
immobilized and free enzyme
45
-
-
invertase III
47
-
-
invertase P-1
50
-
-
enzyme immobilized on bead DEAHP-cellulose
50
-
-
invertase II
50
-
-
enzyme in flow reactor
50
-
-
free enzyme
50
-
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct3
50
-
-
assay at
52
-
-
invertase P-2
55
-
-
enzyme expressed in Pichia pastoris
57
-
-
free enzyme, no significant change upon immobilization
60
-
-
enzyme expressed in Hansenula polymorpha
60
-
-
immobilized enzyme
60
-
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct2
60
-
-
both extracellular and cell extract enzyme
70
-
-
S-form and F-form of invertase
80
-
-
soluble and immobilized S-form, immobilized F-form
90
95
-
10 min assay
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
30
-
active in the range
10
70
-
17% of maximal activity at 10C and at 70C
17
50
-
INVA: same optimal temperature at 30°C, and similar course above that, retaining about 60% activity of the immobilized enzyme at 40°C, about 40% of the free enzyme, below 20% activity retained at 50°C
20
45
-
20C: about 80% of maximal activity, 45C: about 70% of maximal activity
20
55
-
20C: about 25% of maximal activity, 55C: about 45% of maximal activity
20
60
-
immobilized enzyme: about 50% of maximal activity at 20°C, about 40% of maximal activity at 60°C, free enzyme: about 65% of maximal activity at 20°C, about 40% of maximal activity at 50°C, almost no activity at 60°C
20
70
-
20C: about 50% of maximal activity, 70C: about 55% of maximal activity, enzyme expressed in Pichia pastoris
25
45
-
25C: about 45% of maximal activity, 45C: about 45% of maximal activity, fructofuranosidase activity
25
50
-
chimeric fusion enzyme INVA-CBD, immobilized enzyme with higher activities above optimal temperature of 30°C, close to 60% activity at 40°C retained, while in free enzyme only about 20%, about 40% retained at 45-50°C in the immobilized enzyme while only about 20% in the free enzyme, at 25°C immobilized enzyme with about 40% activity, free enzyme with about 70%
25
55
-
25C: about 45% of maximal activity, 50C: about 90% of maximal activity, 55C: about 25% of maximal activity, invertase activity
25
90
B8YJM2
less than 10% activity at 25 and above 80°C, 100% activity at 65-70°C, about 90% activity at 60 and 75°C, about 50% activity at 50-55°C, 10-40% activity at 35-45°C
30
60
-
30C: about 45% of maximal activity, 60C: less than 10% of maximal activity
30
80
-
30C: about 80% of maximal activity of F-form of invertase, about 75% of maximal activity of S-form of invertase, 80C: about 55% of maximal activity of F-form of invertase, 98% of maximal activity of S-form of invertase
30
90
-
30C: about 75% of maximal activity of soluble S-form, about 40% of maximal activity of immobilized S-form, about 60% of maximal activity of immobilized F-form, 90C: about 20% of maximal activity of immobilized S-form, about 15% of maximal activity of soluble S-form, about 50% of maximal activity of immobilized F-form, about 30% of maximal activity of soluble F-form
40
70
-
40C: about 30% of maximal activity, 70C: about 30% of maximal activity
40
70
-
40C: about 55% of maximal activity, 70C: about 60% of maximal activity, enzyme expressed in Hansenula polymorpha
40
75
-
40C: about 40% of maximal activity, 75C: about 50% of maximal activity
40
75
-
about 35% of maximal activity at 40C and at 75C
40
90
-
40C: about 35% of maximal activity, 90C: about 30% of maximal activity
45
50
C0LIF8
-
45
60
-
45C: about 80% of maximal activity, 60C: about 30% of maximal activity
50
80
-
50C: about 50% of maximal activity, 80C: about 70% of maximal activity
60
98
-
60C: about 35% of maximal activity, 98C: about 20% of maximal activity
additional information
-
-
temperature profiles of free and immobilized enzymes
additional information
-
-
temperature profile
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.8
-
-
isoform EINV4
4
-
-
isoform EINV3
4.2
-
-
isoform EINV2
4.4
-
-
enzyme form 1, isoelectric focusing
4.4
-
-
isoform EINV1
4.6
-
-
calculated from sequence
4.7
4.8
-
isoelectrofocusing, S-form and F-form
4.8
-
-
alkaline invertase isozyme IT I, isoelectric focusing
4.9
-
-
calculation from nucleotide sequence
4.9
-
Q9ZTX2
-
4.9
-
A2TLS9
calculated
5.4
-
-
enzyme form 1, isoelectric focusing
5.7
-
Q2XQ19, Q2XQ20, Q2XQ21
isozyme betafruct3, amino acid sequence calculation
5.7
-
-
isoelectric focusing, alkaline invertase
6.5
-
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct3, isoelectric focusing
6.79
-
-
calculated from DNA sequence
7
-
-
isoelectric focusing, acid invertase
7.1
-
-
electrofocusing
7.3
-
Q2XQ19, Q2XQ20, Q2XQ21
isozyme betafruct2, amino acid sequence calculation
7.4
-
-
acid invertase isozyme IT II, isoelectric focusing
8.9
-
Q2XQ19, Q2XQ20, Q2XQ21
isozyme betafruct1, amino acid sequence calculation
9.2
-
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct2, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
invertase I is primarily localized in anthers, invertase II and III are present in much smaller amounts
Manually annotated by BRENDA team
A2X5P7, Q01IS7, Q01IS8
in the coat barely visible expression of OsCIN3, in the embryo very strong expression of OsCIN3, in the endosperm weak expression of OsCIN3; in the coat strong expression of OsCIN1, in the embryo barely visible expression of OsCIN1, in the endosperm no expression of OsCIN1; in the coat weak expression of OsCIN2, in the embryo strong expression of OsCIN2, in the endosperm strong expression of OsCIN2
Manually annotated by BRENDA team
-
invertase F-form and invertase S-form
Manually annotated by BRENDA team
Chenopodium rubrum, Daucus carota
-
-
Manually annotated by BRENDA team
Nicotiana tabacum L. c.v. BY-2
-
-
-
Manually annotated by BRENDA team
-
of single cells from leaf explant
Manually annotated by BRENDA team
-
co-expression of isozyme CWI with inhibitor CIF
Manually annotated by BRENDA team
Nicotiana tabacum L. c.v. BY-2
-
-
-
Manually annotated by BRENDA team
-
a soluble and a cell wall-bound enzyme
Manually annotated by BRENDA team
-
high invertase activity in the apical segment of the coleoptile, the level of activity decreases sharply with distance from the apex
Manually annotated by BRENDA team
-
isoenzyme P-2; microconidia
Manually annotated by BRENDA team
Fusarium oxysporum IAM 5009
-
isoenzyme P-2; microconidia
-
Manually annotated by BRENDA team
C7DY48
of seed. Separate growth of cotyledon types, Cot and Cot E based on a unique differential regeneration response, in vitro on Gamborg's B5 basal nutrient medium, 2% sucrose, and supplemented with N6-benzyladenine. Temporal differences in acid invertase enzyme activity are observed in differentiating explants. In general all N6-benzyladenine levels and both in the presence and/or absence of sucrose, acid invertase levels are lower in Cot than Cot E explants. A higher frequency of regeneration of Cot E explants is positively correlated with higher invertase activity. Invertase gene expression is highest in Cot explants at 12 and 15 days following culture
Manually annotated by BRENDA team
Aspergillus ochraceus TS
-
-
-
Manually annotated by BRENDA team
-
of old cell cultures, isoenzyme P-2
Manually annotated by BRENDA team
Fusarium oxysporum IAM 5009
-
of old cell cultures, isoenzyme P-2
-
Manually annotated by BRENDA team
-
the ability of the maturing embryo to maintain mitotic activity is mediated by metabolic signals of the enzyme from the seed coat
Manually annotated by BRENDA team
-
localization of the cell wall-bound isozyme in the transfer cell layer
Manually annotated by BRENDA team
C4PBL7
VIN activity in elongating fibers is approximately 4-6fold higher than that in leaves, stems, and roots. It is undetectable in fiberless cotton seed epidermis but becomes evident in initiating fibers and remains high during their fast elongation and drops when elongation slows. A genotype with faster fiber elongation has significantly higher fiber VIN activity and hexose levels than a slow-elongating genotype
Manually annotated by BRENDA team
-
low expression level of isozyme CWI, but high expression in androecium and gynoecium development
Manually annotated by BRENDA team
-
co-expression of isozyme CWI with endogenous proteinous inhibitor VIF in flowers
Manually annotated by BRENDA team
-
PaxgINV3 tightly regulated, probably floral-specific cell-wall invertase
Manually annotated by BRENDA team
-
invertase I is primarily localized in anthers, invertase II and II are present at 5% of invertase I activity. Much higher levels of invertase II and III are found in the nonanther organs of the flower
Manually annotated by BRENDA team
-
the vacuolar isozyme Inv-V is important in kernel development, overview
Manually annotated by BRENDA team
-
young and mature
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
barley detectable
Manually annotated by BRENDA team
-
three soluble invertases
Manually annotated by BRENDA team
-
co-expression of isozyme CWIwith endogenous proteinous inhibitor CIF, green and senescent leaves
Manually annotated by BRENDA team
-
co-expression of isozyme CWI with endogenous proteinous inhibitor VIF in green leaves
Manually annotated by BRENDA team
Q94C05, Q94C06
young and mature, expression of isozyme lbbetafruct3 is restricted to shoots and leaves
Manually annotated by BRENDA team
O82119
expression of isoform LIN6 in sink tissues, such as pollen grains and vascular tissues of leaves and stems
Manually annotated by BRENDA team
Aspergillus oryzae IPT-301, Aureobasidium pullulans IPT-329
-
-
-
Manually annotated by BRENDA team
Fusarium oxysporum IAM 5009
-
isoenzyme P-1
-
Manually annotated by BRENDA team
-
cell-wall invertase isozymes CIN1-3, 5, and 8, high expression level of CIN2, plant hormones abscisic acid and gibberellic acid induce cell wall isozyme CIN2 in peduncles antagonizing both peduncle elongation and maintenance of CIN2 transcript levels
Manually annotated by BRENDA team
-
cell wall-bound isozyme Inv-CW has an essential function in carbohydrate supply
Manually annotated by BRENDA team
O82119
expression of isoform LIN6 in sink tissues, such as pollen grains and vascular tissues of leaves and stems
Manually annotated by BRENDA team
A2X5P7, Q01IS7, Q01IS8
in the anther strong expression of OsCIN2, in the ovary strong expression of OsCIN2, in the caryopsis day 4 strong expression of OsCIN2 (strong in vascular parenchyma of chalazal vein, weak in cross-cells, nucellar tissue, endosperm, and lateral vein, and not in pericarp), in the caryopsis day 7 strong expression of OsCIN2 (strong in vascular parenchyma of chalazal vein xylem and aleurone layer, weak in vascular parenchyma of chalazal vein phloem, cross-cells, nucellar projection, inner endosperm, very weakly in pericarp and nucellar epidermis), in the caryopsis day 15 very middle strong expression of OsCIN2, in the caryopsis day 25 almost no expression of OsCIN2; in the anther very strong expression of OsCIN3, in the ovary strong expression of OsCIN3, in the caryopsis day 4 strong expression of OsCIN3 (strong in vascular parenchyma of chalazal vein phloem, cross-cells, nucellar epidermis, endosperm, and lateral vein, weak in vascular parenchyma of chalazal vein xylem, nucellar projection, nucellus, and pericarp), in the caryopsis day 7 strong expression of OsCIN3 (strong in vascular parenchyma of chalazal phloem and aleurone layer, weak in vascular parenchyma of chalazal vein, cross-cells, nucellar projection, epidermis, and endosperm), in the caryopsis day 15 very weak expression of OsCIN3, in the caryopsis day 25 almost no expression of OsCIN3; in the anther weak expression of OsCIN1, in the ovary strong expression of OsCIN1, in the caryopsis day 4 strong expression of OsCIN1, in the caryopsis day 7 strong expression of OsCIN1, in the caryopsis day 15 very weak expression of OsCIN1, and in the caryopsis day 25 almost no expression of OsCIN1
Manually annotated by BRENDA team
-
aged slices of mature roots
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
high expression level of isozyme CWI
Manually annotated by BRENDA team
P49175
-
Manually annotated by BRENDA team
C4PBL7
VIN activity in elongating fibers is approximately 4-6fold higher than that in leaves, stems, and roots
Manually annotated by BRENDA team
-
expression of isozyme Inv-CW in the thin-walled parenchyma of the seed coat, the site of assimilate unloading into the apoplast
Manually annotated by BRENDA team
O82119
expression in germinating seed and seedling
Manually annotated by BRENDA team
-
cell wall invertase INCW2 localizes exclusively to the basal endosperm transfer cells of developing seeds
Manually annotated by BRENDA team
C4PBL7
VIN activity is undetectable in fiberless cotton seed epidermis but becomes evident in initiating fibers
Manually annotated by BRENDA team
-
highest activity at the apex of the coleoptile, much lower in the primary leaf, mesocotyl and root
Manually annotated by BRENDA team
A2X5P7, Q01IS7, Q01IS8
OsCIN3 is more constitutively expressed in all sink and source tissues; strong expression of OsCIN1 (sink and source tissues); strong expression of OsCIN2 (sink tissues)
Manually annotated by BRENDA team
O82119
expression in germinating seed and seedling
Manually annotated by BRENDA team
Q94C05, Q94C06
expression of isozyme lbbetafruct3 is restricted to shoots and leaves
Manually annotated by BRENDA team
-
shoot of etiolated seedling
Manually annotated by BRENDA team
O82119
expression of isoform LIN6 in sink tissues, such as pollen grains and vascular tissues of leaves and stems
Manually annotated by BRENDA team
C4PBL7
VIN activity in elongating fibers is approximately 4-6fold higher than that in leaves, stems, and roots
Manually annotated by BRENDA team
-
cell wall-bound isozyme Inv-CW has an essential function in carbohydrate supply
Manually annotated by BRENDA team
A2X5P7, Q01IS7, Q01IS8
in the shoot strong expression of OsCIN1, in the root strong expression of OsCIN1, in the sink leaf blade strong expression of OsCIN1, in the source leaf blade weak expression of OsCIN1, in the leaf sheath weak expression of OsCIN1, in the internode weak expression of OsCIN1; in the shoot strong expression of OsCIN3, in the root strong expression of OsCIN3, in the sink leaf blade very strong expression of OsCIN3, in the source leaf blade strong expression of OsCIN3, in the leaf sheath strong expression of OsCIN3, in the internode strong expression of OsCIN3; in the shoot weak expression of OsCIN2, in the root weak expression of OsCIN2, in the sink leaf blade strong expression of OsCIN2, in the source leaf blade no expression of OsCIN2, in the leaf sheath no expression of OsCIN2, in the internode strong expression of OsCIN2
Manually annotated by BRENDA team
-
PaxgINV1 exclusively involved in dormancy processes, PaxgINV2 rather in phloem uploading and providing actively growing tissue such as xylem
Manually annotated by BRENDA team
-
whole fly homogenates
Manually annotated by BRENDA team
C4PBL7
VIN activity in elongating fibers is approximately 4-6fold higher than that in leaves, stems, and roots
Manually annotated by BRENDA team
additional information
-
dark-grown Nicotiana tabacum cells transformed with Agrobacterium tumefaciens
Manually annotated by BRENDA team
additional information
Q94C05, Q94C06
isozyme lbbetafruct2 is expressed in most tissues, except for mature leaves and sprouting storage roots, exspecially in sink organs
Manually annotated by BRENDA team
additional information
-
tissue-specific expression of isozymes at flowering and during drought or heat stress, overview
Manually annotated by BRENDA team
additional information
-
culture conditions of wild-type and mutant strains: preferred as carbohydrate and nitrogen sources are wheat bran and corn steep liquor, pH 5.5-6.5, optimal conditions, overview, fermentation kinetics of wild-type and mutant strains with different substrates in submerged fermentations, overview
Manually annotated by BRENDA team
additional information
-
tomato lines with strongly reduced source leaf cell wall-bound invertase activity by RNAi inhibition, 10-15% source leaf activity, while sink leaves and petioles still have 40-60% of wild-type activity, no alterations in other organs and tissue: Lin8-RNAi line 33, line 50, line 57, gene-silencing
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
invertase 2, enzyme can not released by treatment with detergents or high salt concentrations
Manually annotated by BRENDA team
-
acid invertase
Manually annotated by BRENDA team
-
in Schizosaccharomyces pombe the heterologous enzyme is secreted into the cell wall
Manually annotated by BRENDA team
Q2XQ19, Q2XQ20, Q2XQ21
cell wall specific isozyme betafruct1; cell wall specific isozyme betafruct2; cell wall specific isozyme betafruct3
Manually annotated by BRENDA team
-
specific cell wall-bound isozyme
Manually annotated by BRENDA team
Chenopodium rubrum
-
specific cell wall-bound isozyme Cin1
Manually annotated by BRENDA team
-
specific cell wall-bound isozyme
Manually annotated by BRENDA team
Q43799
specific cell wall-bound isozyme
Manually annotated by BRENDA team
Q8L897
specific cell wall-bound isozyme
Manually annotated by BRENDA team
-
specific cell wall-bound isozyme Lin6
Manually annotated by BRENDA team
-
cell wall-bound ivertase isozyme, CWI
Manually annotated by BRENDA team
-
cell wall-bound acid invertase, CWI
Manually annotated by BRENDA team
Chenopodium rubrum
Q42691
cell wall-bound isozyme CIN1
Manually annotated by BRENDA team
-
several cell-wall invertase isozymes CIN1-9
Manually annotated by BRENDA team
Chenopodium rubrum
-
cell wall-bound isozyme Cin1, the isozyme shows tissue-specific co-expression with hexose transporters
Manually annotated by BRENDA team
-
cell wall-bound isozyme CW, the isozyme shows tissue-specific co-expression with hexose transporters
Manually annotated by BRENDA team
A2X5P7, Q01IS7, Q01IS8
-
Manually annotated by BRENDA team
-
two isoforms: Lin6 and Lin8
Manually annotated by BRENDA team
-
wall in growth, about four times more immunostaining in the larger walls in growth than in stunted walls in growth
Manually annotated by BRENDA team
Fusarium oxysporum IAM 5009
-
-
-
Manually annotated by BRENDA team
-
alkaline and acid invertase
Manually annotated by BRENDA team
-
alkaline or neutral isozymes
Manually annotated by BRENDA team
Saccharomyces cerevisiae X
-
rough and smooth
-
Manually annotated by BRENDA team
-
acid invertase
-
Manually annotated by BRENDA team
-
extracellular and intracellular enzyme
-
Manually annotated by BRENDA team
Corynebacterium murisepticum
-
-
-
Manually annotated by BRENDA team
-
when grown on sucrose-containing medium Candida utilis sectretes two invertases: the S-form of 280000 Da and the F-form of 62000 Da
-
Manually annotated by BRENDA team
-
secreted enzyme, 10% of total enzyme amount
-
Manually annotated by BRENDA team
Aspergillus ochraceus TS
-
-
-
-
Manually annotated by BRENDA team
-
trans-Golgi network
Manually annotated by BRENDA team
-
extracellular and intracellular enzyme
Manually annotated by BRENDA team
-
90% of total enzyme amount
Manually annotated by BRENDA team
-
soluble alkaline and acid invertase isozymes, i.e. IT I and IT II
-
Manually annotated by BRENDA team
Q94C05, Q94C06
vacuolar acid invertase; vacuolar acid invertase
-
Manually annotated by BRENDA team
-
soluble acid invertase isozyme, SAI
-
Manually annotated by BRENDA team
-
soluble acid invertase, SAI
-
Manually annotated by BRENDA team
Q94C05, Q94C06
acid invertase; acid invertase
Manually annotated by BRENDA team
-
specific vacuolar isozyme Atbetafruct4
Manually annotated by BRENDA team
-
specific vacuolar isozyme, expressed in green and ripening fruits
Manually annotated by BRENDA team
-
specific vacuolar isozyme
Manually annotated by BRENDA team
-
vacuolar isozyme VIN2
Manually annotated by BRENDA team
P49175
vacuolar isozyme
Manually annotated by BRENDA team
-
acidic or neutral isozymes
Manually annotated by BRENDA team
C4PBL7
isoform VIN1
Manually annotated by BRENDA team
-
highest invertase activities compared to inner or outer membrane, since transport capacity through matrix is limiting enzyme activity, enzyme must be located on inner matrix
Manually annotated by BRENDA team
additional information
-
isozymes with different subcellular localizations
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28000
-
-
gel filtration
28000
-
-
SDS-PAGE
35400
-
-
gel filtration
37000
-
-
gel filtration
47000
-
-
gel filtration and PAGE
48500
-
-
non-denaturing PAGE
51000
-
-
SDS-PAGE
52000
-
-
-
57000
-
-
gel filtration
58000
-
-
gel filtration
59000
-
-
invertase I, gel filtration
60000
-
-
gel filtration
60000
-
-
SDS-PAGE
60000
-
-
native PAGE, SDS-PAGE produced only fragments of 46.3, 27.7, 18.6, and 16.8 kDa, in red fruits additionally a 44 kDa fragment, and a 35 kDa fragment shown to be produced by in planta proteolysis of invertase
62000
-
-
F-form, non-denaturing PAGE
63450
-
-
calculated from DNA sequence
64000
-
-
invertase I, gel filtration
65000
-
-
SDS-PAGE
66000
-
-
neutral invertase, gel filtration
66000
-
-
gel filtration
66000
-
-
isoenzyme AIV I, gel filtration
66000
-
B8YJM2
after treatment with PNGase F representing the unglydosylated enzyme, thus N-linked oligosaccharides appear to represent about 60% (the other part) of the total protein mass
67000
-
-
6xHis-tagged enzyme INVA, 57 kDa from INVA, SDS-PAGE
68000
-
-
isozyme IT II, gel filtration
70100
-
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct2, gel filtration
71000
-
-
theoretical from nanoLC-chip-MS/MS analysis
72000
-
Q9ZTX2
gel filtration
72600
-
Q2XQ19, Q2XQ20, Q2XQ21
recombinant His-tagged isozyme betafruct3, gel filtration
74000
-
-
gel filtration
75000
-
Q0ZR36
SDS-PAGE
75760
-
-
pH 9.4, monomeric enzyme form, gel filtration
76000
-
-
gel filtration
76200
-
-
gel filtration chromatography
78000
-
-
gel filtration
78000
-
-
chimeric fusion enzyme INVA-CBD, 57 kDa from INVA, SDS-PAGE
84000
-
-
gel filtration
84000
-
-
gel filtration
84300
-
Q2PCS4, -
estimated from amino acid sequence
89100
-
-
gel filtration
92000
-
-
gel filtration
94000
-
-
F-invertase, non-denaturing PAGE
95000
-
-
gel filtration
100000
-
-
gel filtration
108000
-
-
invertase II, gel filtration
112500
-
-
pH 8.3, dimeric enzyme form, gel filtration
116000
-
-
invertase IIa, gel filtration
120000
-
-
gel filtration
130000
-
-
gel filtration
130000
-
-
gel filtration
135000
-
-
intracellular enzyme
135000
-
-
gel filtration
155000
-
-
invertase IIb, gel filtration
158000
-
-
gel filtration
158000
-
-
4C, isoenzyme P-2, gel filtration
160000
-
B8YJM2
SDS-PAGE and amino acid sequencing
165000
-
-
gel filtration
170000
-
-
enzyme expressed in Pichia pastoris, gel filtration
185000
-
-
S-invertase, non-denaturing PAGE
190000
-
-
enzyme expressed in Hansenula polymorpha, gel filtration
200000
-
B8YJM2
smear band in activity-stained gel, probably the about 270 kDa glycosylated functional active homodimer
210000
-
-
equilibrium sedimentation centrifugation
216000
-
-
deglycosylated enzyme, gel filtration
220000
-
-
gel filtration
220000
-
-
isozymes SAI and CWI
224000
-
-
25C, isoenzyme P-1, gel filtration
225000
250000
-
non-denaturing PAGE
236000
-
-
gel filtration
240000
-
-
gel filtration
240000
-
-
isozyme IT I, gel filtration
266000
-
-
4C, isoenzyme P-2, gel filtration
270000
-
-
equilibrium sedimentation
270000
-
-
extracellular enzyme, gel filtration
280000
-
-
gel filtration
280000
-
-
S-form, nondenaturing PAGE
280000
-
-
gel filtration
295000
-
-
4C, isonenzyme P-1, gel filtration
300000
-
-
gel filtration
300000
-
-
non-denaturing PAGE
340000
-
-
gel filtration
346000
-
-
native enzyme, gel filtration
380000
-
-
acid invertase, gel filtration
409000
-
-
pH 4.9, tetrameric enzyme form, gel filtration
430000
-
-
TSK Super SW3000 column HPLC
450000
-
-
gel filtration
456000
-
-
neutral invertase, gel filtration
504000
-
-
alkaline invertase, gel filtration
750000
-
-
gel filtration
1070000
-
-
non-denaturing PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 100000, SDS-PAGE
?
-
x * 58000, SDS-PAGE
?
-
x * 53000, SDS-PAGE
?
-
x * 180000, SDS-PAGE
?
-
x * 108000, isoenzyme P-1, SDS-PAGE; x * 84000, isoenzyme P-2, SDS-PAGE