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Literature summary for 3.2.1.26 extracted from

  • Liebl, W.; Brem, D.; Gotschlich, A.
    Analysis of the gene for beta-fructosidase (invertase, inulinase) of the hyperthermophilic bacterium Thermotoga maritima, and characterization of the enzyme expressed in Escherichia coli (1998), Appl. Microbiol. Biotechnol., 50, 55-64.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM Thermotoga maritima
FeCl2
-
Thermotoga maritima
MoCl2 1 mM Thermotoga maritima
additional information high substrate concentrations and also high levels of the cleavage products glucose and fructose do not severly inhibit sucrose inversion Thermotoga maritima
Zn2+ 1 mM ZnCl2 Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15
-
raffinose
-
Thermotoga maritima
19
-
inulin
-
Thermotoga maritima
64
-
sucrose
-
Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
MSB8
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
MSB8
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2390
-
-
Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
Thermotoga maritima fructose + ?
-
?
inulin + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 fructose + ?
-
?
levan + H2O
-
Thermotoga maritima ?
-
?
levan + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
raffinose + H2O
-
Thermotoga maritima fructose + melibiose
-
?
raffinose + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 fructose + melibiose
-
?
sucrose + H2O
-
Thermotoga maritima D-fructose + D-glucose
-
?
sucrose + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 D-fructose + D-glucose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90 95 10 min assay Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 98 60°C: about 35% of maximal activity, 98°C: about 20% of maximal activity Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
14 days, 59% loss of activity Thermotoga maritima
70
-
14 days, 76% loss of activity Thermotoga maritima
80
-
5 h, retains at least 85% of its initial activity Thermotoga maritima
85
-
5 h, 15% loss of activity Thermotoga maritima
90
-
5 h, substantial loss of activity Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
140
-
raffinose
-
Thermotoga maritima
590
-
inulin
-
Thermotoga maritima
2600
-
sucrose
-
Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
recombinant enzyme Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
4.2 7.1 more than 50% of maximal activity between pH 4.2 and pH 7.1 Thermotoga maritima