Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM | Thermotoga maritima | |
FeCl2 | - |
Thermotoga maritima | |
MoCl2 | 1 mM | Thermotoga maritima | |
additional information | high substrate concentrations and also high levels of the cleavage products glucose and fructose do not severly inhibit sucrose inversion | Thermotoga maritima | |
Zn2+ | 1 mM ZnCl2 | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
raffinose | - |
Thermotoga maritima | |
19 | - |
inulin | - |
Thermotoga maritima | |
64 | - |
sucrose | - |
Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
MSB8 | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
MSB8 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2390 | - |
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | - |
Thermotoga maritima | fructose + ? | - |
? | |
inulin + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | fructose + ? | - |
? | |
levan + H2O | - |
Thermotoga maritima | ? | - |
? | |
levan + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
raffinose + H2O | - |
Thermotoga maritima | fructose + melibiose | - |
? | |
raffinose + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | fructose + melibiose | - |
? | |
sucrose + H2O | - |
Thermotoga maritima | D-fructose + D-glucose | - |
? | |
sucrose + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | D-fructose + D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 95 | 10 min assay | Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 98 | 60°C: about 35% of maximal activity, 98°C: about 20% of maximal activity | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
14 days, 59% loss of activity | Thermotoga maritima |
70 | - |
14 days, 76% loss of activity | Thermotoga maritima |
80 | - |
5 h, retains at least 85% of its initial activity | Thermotoga maritima |
85 | - |
5 h, 15% loss of activity | Thermotoga maritima |
90 | - |
5 h, substantial loss of activity | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140 | - |
raffinose | - |
Thermotoga maritima | |
590 | - |
inulin | - |
Thermotoga maritima | |
2600 | - |
sucrose | - |
Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 7.1 | more than 50% of maximal activity between pH 4.2 and pH 7.1 | Thermotoga maritima |