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Literature summary for 3.2.1.26 extracted from
- Purification and properties of invertase from Portulaca oleracea plant (2004), Egypt. J. Biotechnol., 16, 317-330.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight inhibition | Portulaca oleracea |  |
| Cd2+ | strong inhibition | Portulaca oleracea | |
| Co2+ | strong inhibition | Portulaca oleracea | |
| Cu2+ | strong inhibition | Portulaca oleracea | |
| EDTA | slight inhibition | Portulaca oleracea | |
| Fe2+ | strong inhibition | Portulaca oleracea | |
| Hg2+ | strong inhibition | Portulaca oleracea | |
| K+ | strong inhibition | Portulaca oleracea | |
| Mg2+ | slight inhibition | Portulaca oleracea | |
| Mn2+ | strong inhibition | Portulaca oleracea | |
| Na+ | strong inhibition | Portulaca oleracea | |
| Pb2+ | strong inhibition | Portulaca oleracea | |
| Zn2+ | slight inhibition | Portulaca oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, SDS-PAGE | Portulaca oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | Portulaca oleracea | high activity on sugar cane bagasse, soybean waste, and wheat bran, saccharification levels are 93.69, 57.67, and 55.24, respectively | D-glucose + D-fructose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Portulaca oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 53.4fold by ammonium sulfate fractionation, and two steps of gel filtration to homogeneity | Portulaca oleracea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 158.2 | - |
purified native enzyme | Portulaca oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | preferred substrate | Portulaca oleracea | D-glucose + D-fructose | - |
? | |
| sucrose + H2O | high activity on sugar cane bagasse, soybean waste, and wheat bran, saccharification levels are 93.69, 57.67, and 55.24, respectively | Portulaca oleracea | D-glucose + D-fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Portulaca oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| invertase | - |
Portulaca oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Portulaca oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
1 h, purified enzyme, pH 5.0, stable | Portulaca oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Portulaca oleracea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
1 h, purified enzyme, 40°C, stable | Portulaca oleracea |
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