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Literature summary for 3.2.1.26 extracted from

  • Lee, H.S.; Sturm, A.
    Purification and characterization of neutral and alkaline invertase from carrot (1996), Plant Physiol., 112, 1513-1522.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.26

Inhibitors

Inhibitors Comment Organism Structure
ATP inhibition of neutral invertase, no inhibition of alkaline invertase Daucus carota
Ca2+ CaCl2; inhibition of alkaline invertase no inhibition of neutral invertase Daucus carota
CTP inhibition of neutral invertase, no inhibition of alkaline invertase Daucus carota
fructose competitive inhibitor of neutral and alkaline enzyme Daucus carota
glucose non-competitive inhibitor of neutral and alkaline invertase Daucus carota
GTP inhibition of neutral invertase, no inhibition of alkaline invertase Daucus carota
Hg2+ no inhibition of neutral and alkaline invertase Daucus carota
Mg2+ MgCl2, inhibition of alkaline invertase no inhibition of neutral invertase Daucus carota
Mn2+ MnCl2, inhibition of alkaline invertase no inhibition of neutral invertase Daucus carota
UTP inhibition of neutral invertase, no inhibition of alkaline invertase Daucus carota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14.3
-
 sucrose pH 6.8, neutral invertase Daucus carota
15.1
-
 sucrose pH 8.0, alkaline invertase Daucus carota
18.7
-
 sucrose pH 7.5, neutral invertase Daucus carota
20
-
 sucrose neutral and alkaline invertase Daucus carota
20.7
-
 raffinose pH 7.5, alkaline invertase Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 8 * 57000, neutral invertase, SDS-PAGE Daucus carota
126000
-
 4 * 126000, alkaline invertase, SDS-PAGE Daucus carota
456000
-
 neutral invertase, gel filtration Daucus carota
504000
-
 alkaline invertase, gel filtration Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Daucus carota

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Daucus carota

Storage Stability

Storage Stability Organism
4°C, 50% glycerol, stable for several days Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raffinose + H2O no activity with alkaline invertase, hydrolysis with neutral invertase Daucus carota fructose + melibiose
-
 ?
stachyose + H2O no activity with alkaline invertase, hydrolysis with neutral invertase Daucus carota ?
-
 ?
sucrose + H2O alkaline invertase is highly specific for sucrose, neutral invertase also hydrolyzes raffinose and stachyose Daucus carota D-fructose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 57000, neutral invertase, SDS-PAGE Daucus carota
tetramer 4 * 126000, alkaline invertase, SDS-PAGE Daucus carota

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40
-
 Daucus carota

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 rapid loss of activity above Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 neutral invertase Daucus carota
8
-
 alkaline invertase Daucus carota

pH Range

pH Minimum pH Maximum Comment Organism
4.8 8 pH 4.8: about 35% of maximal activity, pH 8.0: about 60% of maximal activity, neutral invertase Daucus carota
7 9 pH 7.0: about 50% of maximal actiovity, pH 9.0: about 70% of maximal activity, alkaline invertase Daucus carota

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 alkaline and neutral invertase, stable in presence of protease inhibitors Daucus carota