General Stability | Organism |
---|---|
enzyme embedded within a polyacrylamide gel and freeze dried retains only 25% of their original activity | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.1 mM, 95% inhibition of the CM-cellulose adsorbed enzyme, enzyme embedded within a polyacrylamide gel is inhibited 72.7% | Solanum lycopersicum | |
Cu2+ | CuSO4 | Solanum lycopersicum | |
PCMB | 0.1 mM, 94% inhibition of the CM-cellulose adsorbed enzyme, enzyme embedded within a polyacrylamide gel is inhibited 16.7% | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
sucrose | enzyme embedded within a polyacrylamide gel | Solanum lycopersicum | |
6.9 | - |
sucrose | enzyme adsorbed on CM-cellulose | Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K4Fe(CN)6 | activates enzyme embedded within a polyacrylamide gel, no effect on CM-cellulose-bound enzyme | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Solanum lycopersicum | D-fructose + D-glucose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
5 min, complete inactivation of the CM-cellulose adsorbed enzyme | Solanum lycopersicum |
70 | - |
5 min, enzyme embedded within a polyacrylamide gel loses 85% loss of activity | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
free enzyme and enzyme embedded within a polyacrylamide gel | Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.3 | 6 | pH 3.3: about 50% of maximal activity of the free enzyme, about 70% of maximal activity of the enzyme embedded within a polyacrylamide gel, pH 6.0: about 10% of activity of the free enzyme, about 45% of activity of the enzyme embedded within a polyacrylamide gel | Solanum lycopersicum |