EC Number   |
Organic Solvent |
Reference |
|---|
    3.2.1.26 | Glycerol |
addition of up to 20% glycerol does not significantly alter INV1p stability |
682030 |
| 3.2.1.26 | Glycerol |
no significant increase in alpha-helical content of invertase with 30% glycerol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-6% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 79, 82, 85, and 84 degrees Celsius with 10, 20, 30, and 40% glycerol, stability increase due to hydration |
701080 |
| 3.2.1.26 | more |
with sorbitol no significant increase in alpha-helical content of invertase with 20% sorbitol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-5%, at pH 9 increases of alpha-helical content from 3-6% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 77, 79, 81, and 82 degrees Celsius with 10, 20, 30, 40% sorbitol, respectively, and from 70 to 72 and 73 degrees Celsius with 30 and 40% sorbitol, respectively, at pH 9, stability increase due to hydration |
701080 |
| 3.2.1.26 | more |
xylitol with no significant increase in alpha-helical content of invertase with 25% xylitol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-6%, at pH 9 increases of alpha-helical content from 3-7% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 79, 82, and 84 degrees Celsius with 10, 20, and 30-40% xylitol and from 70 to 73, 75, 77 degrees Celsius with 20, 30, 40% xylitol at pH 9, stability increase due to hydration |
701080 |
| 3.2.1.26 | urea |
8 M urea at 20°C with a half-life of 74 min |
700787 |
