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Literature summary for 3.2.1.26 extracted from
- Purification, kinetic and thermodynamic characterization of soluble acid invertase from sugarcane (Saccharum officinarum L.) (2009), Plant Physiol. Biochem., 47, 188-194.
General Stability
| General Stability | Organism |
|---|---|
| half-life in response to chymotrypsin attack: 315 min | Saccharum officinarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 160.6 | - |
sucrose | pH 5.0, 50°C, 55 mg/ml | Saccharum officinarum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE | Saccharum officinarum |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | 8 M urea at 20°C with a half-life of 74 min | Saccharum officinarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharum officinarum | - |
salt sensitive cultivar CP 77-400 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, dialysis, Hiload anion exchange chromatography, elution at about 640 mM NaCl, hydrophobic interaction chromatography with phenyl superose column, and gel filtration chromatography on a Pharmacia FPLC unit | Saccharum officinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | - |
Saccharum officinarum | D-fructose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, native and subunit molecular mass almost identical on SDS-PAGE | Saccharum officinarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SAI | soluble acid invertase | Saccharum officinarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Saccharum officinarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
half-life 112 min | Saccharum officinarum |
| 55 | - |
half-life 240 min | Saccharum officinarum |
| 63 | - |
half-life 10 min | Saccharum officinarum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21 | - |
sucrose | pH 5.0, 50°C | Saccharum officinarum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
- |
Saccharum officinarum |
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