Literature summary for 3.2.1.26 extracted from

Giraldo, M.A.; Goncalves, H.B.; Furriel, R.d.o.s..P.; Jorge, J.A.; Guimaraes, L.H.
Characterization of the co-purified invertase and beta-glucosidase of a multifunctional extract from Aspergillus terreus (2014), World J. Microbiol. Biotechnol., 30, 1501-1510.

Search for more literature for 3.2.1.26

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	134.4% activity at 1 mM	Aspergillus terreus
NaBr	130.9% activity at 1 mM	Aspergillus terreus
NaCl	120% activity at 1 mM	Aspergillus terreus
NH4F	130.5% activity at 1 mM	Aspergillus terreus
Zn(NO3)2	155.4% activity at 1 mM	Aspergillus terreus

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	87.8% residual activity at 10 mM	Aspergillus terreus
Ag+	18% residual activity at 10 mM	Aspergillus terreus
Al3+	20.5% residual activity at 10 mM	Aspergillus terreus
Co2+	63.5% residual activity at 10 mM	Aspergillus terreus
Cu2+	28.5% residual activity at 10 mM	Aspergillus terreus
EDTA	47.9% residual activity at 10 mM	Aspergillus terreus
Fe2+	11.6% residual activity at 10 mM	Aspergillus terreus
Fe3+	16.5% residual activity at 10 mM	Aspergillus terreus
Hg+	complete inhibition at 10 mM	Aspergillus terreus
K+	67.1% residual activity at 10 mM	Aspergillus terreus
NaBr	62.7% residual activity at 10 mM	Aspergillus terreus
NaH2PO4	67.9% residual activity at 10 mM	Aspergillus terreus
NH4Cl	36.6% residual activity at 10 mM	Aspergillus terreus
NH4F	51.3% residual activity at 10 mM	Aspergillus terreus
Zn(NO3)2	66.2% residual activity at 10 mM	Aspergillus terreus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
11.9	-	sucrose	in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C	Aspergillus terreus
22.5	-	cellobiose	in 100 mM sodium acetate buffer, pH 5.0, at 50°C	Aspergillus terreus
22.7	-	sucrose	in 100 mM sodium acetate buffer, pH 5.0, at 50°C	Aspergillus terreus
123.6	-	cellobiose	in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C	Aspergillus terreus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ba2+	136% activity at 1 mM	Aspergillus terreus
Ca2+	145% activity at 10 mM	Aspergillus terreus
Co2+	168% activity at 1 mM	Aspergillus terreus
Fe3+	117% activity at 1 mM	Aspergillus terreus
K+	124% activity at 1 mM	Aspergillus terreus
Mg2+	161% activity at 1 mM	Aspergillus terreus
Mn2+	261% activity at 1 mM	Aspergillus terreus
Zn2+	126% activity at 1 mM	Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26900	-	1 * 46700 + 1 * 26900, SDS-PAGE	Aspergillus terreus
46700	-	1 * 46700 + 1 * 26900, SDS-PAGE	Aspergillus terreus
74800	-	gel filtration	Aspergillus terreus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus terreus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose column chromatography and Sephacryl S-200 gel filtration	Aspergillus terreus

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:rye flour-grown cell	-	Aspergillus terreus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.9	-	crude extract, with sucrose as substrate, in 100 mM sodium acetate buffer, pH 5.0, at 50°C	Aspergillus terreus
555	-	after 139.4fold purification, with sucrose as substrate, in 100 mM sodium acetate buffer, pH 5.0, at 50°C	Aspergillus terreus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellobiose + H2O	-	Aspergillus terreus	?	-	?
inulin + H2O	worst substrate	Aspergillus terreus	?	-	?
additional information	no activity with maltose, starch, and trehalose	Aspergillus terreus	?	-	?
raffinose + H2O	-	Aspergillus terreus	D-fructose + melibiose	-	?
sucrose + H2O	best substrate	Aspergillus terreus	D-fructose + D-glucose	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 46700 + 1 * 26900, SDS-PAGE	Aspergillus terreus

Synonyms

Synonyms	Comment	Organism
invertase	-	Aspergillus terreus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
55	65	-	Aspergillus terreus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	70	the enzyme remains stable for 1 h at 55°C when diluted in water. The extracellular invertase is fully stable at lower temperatures, but half-lives of 40 and 5 min are estimated at 60 and 70°C, respectively	Aspergillus terreus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	6	-	Aspergillus terreus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	10	the enzyme remains stable for 1 h	Aspergillus terreus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
1	-	in 100 mM sodium acetate buffer, pH 5.0, at 50°C	Aspergillus terreus	Hg+

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Release 2023.1 (January 2023)