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Literature summary for 3.2.1.26 extracted from

  • Goosen, C.; Yuan, X.L.; van Munster, J.M.; Ram, A.F.; van der Maarel, M.J.; Dijkhuizen, L.
    Molecular and biochemical characterization of a novel intracellular invertase from Aspergillus niger with transfructosylating activity (2007), Eukaryot. Cell, 6, 674-681.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and in an invertase-negative strain of Saccharomyces cerevisiae Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
sucrose increase of the sucrose concentration from 2.5 mM to 1 M results in decreased hydrolysis activity Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 sucrose in 50 mM acetate buffer (pH 5.0) at 37°C Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger Q0ZR36 putative intracellular invertase
-

Purification (Commentary)

Purification (Comment) Organism
His tag affinity chromatography Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
 Aspergillus niger D-fructose + ?
-
 ?
additional information does not use inulin and levan as substrates Aspergillus niger ?
-
 ?
nystose + H2O
-
 Aspergillus niger D-fructose + ?
-
 ?
sucrose + H2O
-
 Aspergillus niger D-glucose + D-fructose
-
 ?

Synonyms

Synonyms Comment Organism
invertase
-
 Aspergillus niger
SucB shows also transfructosylation activity of 20 to 30% of total activity at sucrose concentrations up to 400 mM Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 at 50°C or higher, no activity can be detected, whereas at lower temperatures, the SucB specific activity remains relatively high, with 50% activity remaining at 25°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
268.1
-
 sucrose in 50 mM acetate buffer (pH 5.0) at 37°C Aspergillus niger