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Literature summary for 3.2.1.26 extracted from
- Purification and characterization of invertase isoenzymes from Fusarium oxysporum (1980), Agric. Biol. Chem., 44, 489-498.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Fusarium oxysporum |  |
| Hg2+ | - |
Fusarium oxysporum | |
| PCMB | - |
Fusarium oxysporum | |
| SDS | - |
Fusarium oxysporum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
sucrose | invertase P-2 | Fusarium oxysporum | |
| 4.4 | - |
sucrose | invertase P-1 | Fusarium oxysporum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
x * 84000, isoenzyme P-2, SDS-PAGE | Fusarium oxysporum |
| 108000 | - |
x * 108000, isoenzyme P-1, SDS-PAGE | Fusarium oxysporum |
| 158000 | - |
4°C, isoenzyme P-2, gel filtration | Fusarium oxysporum |
| 224000 | - |
25°C, isoenzyme P-1, gel filtration | Fusarium oxysporum |
| 266000 | - |
4°C, isoenzyme P-2, gel filtration | Fusarium oxysporum |
| 295000 | - |
4°C, isonenzyme P-1, gel filtration | Fusarium oxysporum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium oxysporum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | isoenzyme P-1 contains 36% carbohydrate, isoenzyme P-2 contains 23% carbohydrate. Predominantly mannose with a smaller percentage of glucose, galactose, N-acetylglucosamine and N-acetylgalactosamine | Fusarium oxysporum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fusarium oxysporum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | isoenzyme P-2 | Fusarium oxysporum | - |
| mycelium | isoenzyme P-1 | Fusarium oxysporum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Fusarium oxysporum |
| 420 | - |
isoenzyme P-1 | Fusarium oxysporum |
| 455 | - |
isoenzyme P-2 | Fusarium oxysporum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raffinose + H2O | - |
Fusarium oxysporum | fructose + melibiose | - |
? | |
| sucrose + H2O | - |
Fusarium oxysporum | D-fructose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 84000, isoenzyme P-2, SDS-PAGE | Fusarium oxysporum |
| ? | x * 108000, isoenzyme P-1, SDS-PAGE | Fusarium oxysporum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
invertase P-1 | Fusarium oxysporum |
| 52 | - |
invertase P-2 | Fusarium oxysporum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
10 min, complete loss of activity of isoenzyme P-1, 20% loss of activity of isoenzyme P-2 | Fusarium oxysporum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
invertase P-1 | Fusarium oxysporum |
| 5 | - |
invertase P-2 | Fusarium oxysporum |
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