Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.26 extracted from

  • Giraldo, M.; da Silva, T.; Salvato, F.; Terenzi, H.; Jorge, J.; Guimaraes, L.
    Thermostable invertases from Paecylomyces variotii produced under submerged and solid-state fermentation using agroindustrial residues (2012), World J. Microbiol. Biotechnol., 28, 463.
    View publication on PubMed
Search for more literature for 3.2.1.26

Application

Application Comment Organism
synthesis production of high levels of cell extract and extracellular invertases when grown under submerged fermentation and solid-state fermentation, using agroindustrial products or residues as substrates, mainly soy bran and wheat bran, at 40°C for 72 h and 96 h, respectively. Addition of glucose or fructose in submerged fermentation inhibits enzyme production, while the addition of 1% (w/v) peptone as organic nitrogen source enhances the production by 3.7fold. 1% (w/v) (NH4)2HPO4 inhibits enzyme production around 80% Paecilomyces variotii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 sucrose extracellular enzyme, pH 4.5, 60°C Paecilomyces variotii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Paecilomyces variotii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM enhances the extracellular activity about 154%, and enhances the intracellular activity about 202% Paecilomyces variotii
Mg2+ 1 mM enhances the extracellular activity about 180% Paecilomyces variotii
Mn2+ 1 mM enhances the intracellular activity about 317% Paecilomyces variotii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
 1 * 210000, SDS-PAGE of extracellular enzyme Paecilomyces variotii

Organism

Organism UniProt Comment Textmining
Paecilomyces variotii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content of 53.6%, extracellular enzyme Paecilomyces variotii

Purification (Commentary)

Purification (Comment) Organism
extracellular isoform Paecilomyces variotii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
601
-
 extracellular enzyme, pH 4.5, 60°C Paecilomyces variotii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Paecilomyces variotii alpha-D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 210000, SDS-PAGE of extracellular enzyme Paecilomyces variotii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 both extracellular and cell extract enzyme Paecilomyces variotii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable for 1 h, both extracellular and cell extract enzyme Paecilomyces variotii
70
-
 half-life 10 min, both extracellular and cell extract enzyme Paecilomyces variotii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5 both extracellular and intracellular enzyme Paecilomyces variotii