Cloned (Comment) | Organism |
---|---|
expression in Hansenula polymorpha and Pichia pastoris | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
enzyme expressed in Pichia pastoris, gel filtration | Saccharomyces cerevisiae |
190000 | - |
enzyme expressed in Hansenula polymorpha, gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | commercial enzyme contains 50% carbohydrate, enzyme expressed in Hansenula polymorpha contains 35% and enzyme expressed in Pichia pastoris contains 25% carbohydrate | Saccharomyces cerevisiae |
Renatured (Comment) | Organism |
---|---|
reactivation upon heating at 80°C depends on protein concentration. Irreversible denaturation is observed for carbohydrate-depleted enzyme | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
enzyme expressed in Hansenula polymorpha | Saccharomyces cerevisiae |
3.4 | - |
enzyme expressed in Pichia pastoris | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Saccharomyces cerevisiae | beta-D-fructose + alpha-D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
enzyme expressed in Pichia pastoris | Saccharomyces cerevisiae |
60 | - |
enzyme expressed in Hansenula polymorpha | Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: about 50% of maximal activity, 70°C: about 55% of maximal activity, enzyme expressed in Pichia pastoris | Saccharomyces cerevisiae |
40 | 70 | 40°C: about 55% of maximal activity, 70°C: about 60% of maximal activity, enzyme expressed in Hansenula polymorpha | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
180 min, more than 90% of initial activity of enzyme expressed in Hansenula polymorpha and Pichia pastoris remains | Saccharomyces cerevisiae |
60 | - |
10 min, enzyme expressed in Hansenula polymorpha shows optimal stability towards thermal inactivation at pH 5.5. Enzyme expressed in Pichia pastoris shows optimal stability towards thermal inactivation at pH 6 | Saccharomyces cerevisiae |