Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 41% activation at 1 mM | Bifidobacterium animalis subsp. lactis | |
DTT | 26% activation at 1 mM | Bifidobacterium animalis subsp. lactis | |
EDTA | 32% activation at 1 mM | Bifidobacterium animalis subsp. lactis |
Cloned (Comment) | Organism |
---|---|
gene cscA, genetic organization, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue and as soluble, His-tagged enzyme in strain BL21(DE3) | Bifidobacterium animalis subsp. lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | complete inhibition at 1 mM | Bifidobacterium animalis subsp. lactis | |
Ca2+ | 15% inhibition at 1 mM | Bifidobacterium animalis subsp. lactis | |
Co2+ | 38% inhibition at 1 mM | Bifidobacterium animalis subsp. lactis | |
Cu2+ | complete inhibition at 1 mM | Bifidobacterium animalis subsp. lactis | |
Fe2+ | 55% inhibition at 1 mM | Bifidobacterium animalis subsp. lactis | |
SDS | complete inhibition at 1 mM | Bifidobacterium animalis subsp. lactis | |
Zn2+ | 56% inhibition at 1 mM | Bifidobacterium animalis subsp. lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bifidobacterium animalis subsp. lactis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by 1 mM Mn2+ | Bifidobacterium animalis subsp. lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant His-tagged enzyme, SDS-PAGE | Bifidobacterium animalis subsp. lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium animalis subsp. lactis | Q564C7 | gene cscA, strain DSM 10140 | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble, His-tagged CscA 238fold from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Bifidobacterium animalis subsp. lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bifidobacterium animalis subsp. lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
levan + H2O | low activity | Bifidobacterium animalis subsp. lactis | D-fructose + ? | - |
? | |
additional information | no activity with melezitose, raffinose is a poor substrate | Bifidobacterium animalis subsp. lactis | ? | - |
? | |
Raftiline HP + H2O | low activity | Bifidobacterium animalis subsp. lactis | D-glucose + D-fructose | - |
? | |
raftiline LS + H2O | - |
Bifidobacterium animalis subsp. lactis | D-glucose + D-fructose | - |
? | |
raftilose + H2O | best substrate for CscA | Bifidobacterium animalis subsp. lactis | D-fructose | - |
? | |
sucrose + H2O | low activity | Bifidobacterium animalis subsp. lactis | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant His-tagged enzyme, SDS-PAGE | Bifidobacterium animalis subsp. lactis |
Synonyms | Comment | Organism |
---|---|---|
beta-(1-2)-fructofuranosidase | - |
Bifidobacterium animalis subsp. lactis |
CscA | - |
Bifidobacterium animalis subsp. lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bifidobacterium animalis subsp. lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Bifidobacterium animalis subsp. lactis |